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Cells isolated from cryopreserved amniotic membrane. The cells have a round morphology; during 7 days of cultivation, no growth was observed (magnification ×200)
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Amniotic membrane is a biological material widely used in plastic and reconstructive surgery and in ophthalmology. Due to its excellent biocompatibility and strength we tried to use it as a scaffold for the in vitro cultivation of different cell types, especially keratinocytes and limbal stem cells. It was possible to cultivate limbal stem cells an...
Citations
... . AM can be used at most 14 days after harvesting when stored at + 4 °C, but because donor testing must be finished first, it`s unsuitable to be used immediately 5 . There, it is more profitable to preserve AM using one of the three prevalent methods: cryopreservation, using dimethyl sulfoxide (DMSO) as cryoprotectant 18 , glycerolization, eliminating all pathogens and 85% glycerol at + 4 °C, was shown to obliterate the HIV-1 virus 19 , and lyophilization 20 . ...
... There, it is more profitable to preserve AM using one of the three prevalent methods: cryopreservation, using dimethyl sulfoxide (DMSO) as cryoprotectant 18 , glycerolization, eliminating all pathogens and 85% glycerol at + 4 °C, was shown to obliterate the HIV-1 virus 19 , and lyophilization 20 . At the Bratislava Central Tissue Bank (CTB) was developed method for cryopreserving AM using polyester net, membranes preserved with this method has shown outstanding outcomes when used in ophthalmology, burn medicine, or plastic surgery 5 . Cryopreservation of AM better preserves basement membrane components so it`s more suitable for cell cultivation, because they release more soluble wound-healing-modulating factors compared to other methods of preservation 21 . ...
The amniotic membrane (AM) has special properties, making it ideal for clinical applications in various surgical fields like ophthalmology. It is used more frequently to cover conjunctival and corneal defects. In our retrospective study we have been combined 68 patients with epibulbar conjunctival tumors they have been surgically treated in the period of 2011–2021. Seven (10.3%) patients have been treated with AM application after surgical removal of the tumor. 54 (79%) cases were malignant, and 14 (21%) were benign. In the analyzed dataset the males had just slightly higher chance of malignancy than females, 80% versus 78.3%. For the significancy calculation the Fisher exact test was used and the result proved no significancy (p = 0.99). Six patients with AM application were malignant. The observed difference in the number of quadrants of the bulbar conjunctiva infiltrated versus significant malignancy with p = 0.050 calculated by Fisher Exact test and with p = 0.023 calculated by Likelihood-ratio test. The results of our study indicate that AM grafts are an effective alternative to cover defects after removal of epibulbar lesions due to their anti-inflammatory properties because the conjunctiva must be preserved, and especially the most important application is in malignant epibulbar conjunctival tumors.
... In the last years, it has been studied for the repair of peripheral nerve defects (Bourgeois et al 2019, Zhang et al 2019, full-thickness skin (Song et al 2017), wound diabetic ulcerations (Paggiaro et al 2018), ophthalmological conditions (Navas et al 2018), burns (Puyana et al 2020), intrauterine adhesion due to endometrial fibrosis (Chen et al 2019), osteoinductive (Tang et al 2018), cartilage grafts (Motamed et al 2019), soft tissue filling (Buday and Ozturk 2019), associated with colistin and silver nanoparticles to treat infected burns in animal model (Wali et al 2022), among others. In addition, its application as a scaffold for cells (Dragúňová et al 2019), as drug-delivery systems (Mencucci et al 2011) and organ-on-chip technology (Richardson et al 2020) are promising for clinical therapies. ...
... MSCs associated with decellularized hAM (DhAM) have been studied in several in vitro and in vivo models. In vitro studies showed that DhAM can be used to grow MSCs derived from human tissues as from dental pulp, iliac crest bone-marrow (Echarte et al 2022) human adipose tissue-derived stem cells (Gholipourmalekabadi et al 2016, Naasani et al 2019, Hadipour et al 2021, sclerocorneal limbus (Naasani et al 2018) and epithelial cells (Dragúňová et al 2019). The DhAM was combined with bone marrow MSCs to investigate the wound healing effects in full thickness skin defects in rabbits (Kim et al 2009). ...
Therapies to deep burn injuries remain a global challenge. Human amniotic membrane (hAM) is a biomaterial that has been increasingly explored by the field of regenerative medicine. A decellularized hAM (DhAM) can be used as scaffold for mesenchymal stromal cells (MSCs) to grow without the loss of their stemness potential, allowing its application as cell therapy for wound healing. In this work, we associated DhAM with adipose-derived mesenchymal stromal cells (DhAM+AD-MSC), as a therapy strategy for second-degree burns in a preclinical model . Animals with induced second-degree burns were divided into 4 groups: control, which consists of a non-adherent gauze; a synthetic commercial dressing as the positive control (control+); DhAM; and DhAM plus rat AD-MSCs (DhAM+AD-MSCs), followed by detailed and long term analysis (5 weeks). The macroscopical analysis showed the healing improvement in the wound area after the DhAM+AD-MSC treatment. Histological analysis also showed no alteration in the animal organs and a regular epithelial progression in comparison to the control. This observation was also confirmed by the analysis of suprabasal layers in the neoepidermis with CK10, showing a stratified and differentiated epithelium, when compared to Control and Control+. A strong CD73 (ecto-5′-nucleotidase) labeling was observed in the first two weeks postburn in dermis and epidermis. The expression in dermis was stronger in the second week in the middle of the wound, when comparing the Control+ with DhAM+AD-MSCs (p=0.0238). In the epidermis the expression of CD73 was increased in all regions when compared to the control. This data suggests the involvement of this protein on wound healing. A low CD11b labeling was observed in DhAM+AD-MSCs treatment group mainly in the last treatment week, in comparison to Control and Control+ (p<0.0001), which indicates a reduction in the inflammatory process. MSCs through CD73 can release high concentrations of adenosine, an immunosuppressive molecule, suggesting that this could be the mechanism by which the inflammation was better modulated in the DhAM+AD-MSCs group. The results obtained with this preclinical model confirm the effectiveness and safety of this low-cost and highly available dressing for future clinical application as a therapy for burn treatments.
... The outer layer, the avascular stroma, contains mostly mesenchymal stromal cells and collagen I, III, IV, and VI that increase it`s tensile strength 4 . The AM has special properties, making it ideal for clinical applications in various surgical elds such as urology, ophthalmology, oncology and plastic surgery 5 . ...
The amniotic membrane (AM) has special properties, making it ideal for clinical applications in various surgical fields like ophthalmology. It is used more frequently to cover conjunctival and corneal defects. In our retrospective study we have been combined 68 patients with epibulbar conjunctival tumors they have been surgically treated in the period of 2011-2021. Seven (10.3%) patients have been treated with AM application after surgical removal of the tumor. 54 cases were malignant and 14 cases were benign. In the analyzed dataset the males had just slightly higher chance of malignancy than females, 80% versus 78.3%. For the significancy calculation the Fisher exact test was used and the result proved no significancy (p=0.99). Six patients with AM application were malignant. The observed difference in the number of quadrants of the bulbar conjunctiva infiltrated versus significant malignancy with p=0.050 calculated by Fisher Exact test and with p=0.023 calculated by Likelihood-ratio test. The results of our study indicate that AM grafts are an effective alternative to cover defects after removal of epibulbar lesions due to their anti-inflammatory properties because the conjunctiva must be preserved, and especially the most important application is in malignant epibulbar conjunctival tumors.
... Later on, J Dragunova et al. in their study showed that amniotic membrane can be a good scaffold for human keratinocytes and corneal limbal stem cells. 17 (2) Allogenic SLET: Extensive ocular surface damages (Dua's classification grade 4 and beyond) often result in delayed corneal epithelial healing. This is because of the fact that the unaffected island of conjunctival epithelium lies in the fornices and they populate the cornea last. ...
Purpose: To review the surgical management options in ocular chemical burn, including newer advances.
Methods: Online literature search of published articles over last 5 years on surgical management of ocular chemical burn and newer advances were performed on December 30, 2020.
Results: Following literature search and screening using adequate filters, 67 review articles on surgical management of ocular chemical burns were retrieved. The review talks about the surgical management options starting from Debridement in acute stage to various visual rehabilitative procedures in the chronic stage. The review also highlights the evolving surgical advances in this field.
Conclusion: It is imperative to choose adequate surgical tool wherever applicable; current review discusses the role of each surgical option at different clinical stages in detail.
