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Canonical discriminant analysis of Microglossum viride (white) and M. griseoviride (black) collections based on 16 morphological characters (see TABLE I).  

Canonical discriminant analysis of Microglossum viride (white) and M. griseoviride (black) collections based on 16 morphological characters (see TABLE I).  

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Studies in Microglossum viride (Pers.) Gillet revealed that the name was used incorrectly for two similar but different taxa. Analyses of morphological, ecological and molecular (sequences of ITS and LSU region of rRNA gene) characters of type and voucher specimens of M. viride and related taxa resulted in delimitation and description of a new spec...

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... The finding was confirmed by the high similarity of ITS (100%) with the sequence of the type specimen of this species NR_132026 (Kučera et al., 2014). General distribution: North and South America, Europe, Southeast Asia (Japan and China), Australia (Mic-roglossum…, 2022). ...
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First records for Russia of naviculoid diatom from the Yaroslavl Region, and micromycetes from the Republic of North Ossetia — Alania, green alga for the Leningrad Region and Yamal-Nenets Autonomous Area, fragilarioid diatom for the Kaliningrad Region, red alga for the Nizhny Novgorod Region, cyanoprokaryota for the Leningrad Region and Chukotka Autonomous Okrug, and crustaceous red alga for the Autonomous Republic of Adjara of Georgia, macromycetes for the Leningrad Region, Khanty-Mansi Autonomous Area — Yugra, Republic of Tuva, Trans-Baikal Territory, myxomycetes for the Trans-Baikal Territory, lichens and allied fungi for the Murmansk and Tver regions, republics of Karelia and Tuva, Yamal-Nenets Autonomous Area, Altai and Khabarovsk territories, cyanolichen for the Urals and the Orenburg Region, mosses for the Lipetsk Region, republics of Ingushetia and Buryatia, Krasnoyarsk and Trans-Baikal territories are presented. The data on their localities, habitats, distribution are provided. The specimens are kept in the herbaria ALTB, GSU, IBIW, IRK, KPABG, LE, MHA, MW, NNSU, NSK, PZV, TBI, UUH, VU, YSU, and the Diatom collection of the Laboratory for Algology of IBIW RAS. Sequences of 16S, and 16S–23S ITS cyanobacterial RNA regions, ITS1-5.8S-ITS2 fungal and ITS1-2 moss nrDNA regions of some specimens have been deposited in the GenBank.
... [3,28] and Microglossum spp. belonging to Geoglossaceae [29,30]. In the genus Entoloma, all species from the subgenera Leptonia and Trichopilus as well as some from the subgenus Nolanea are regarded as grassland dwellers [25,31]. ...
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... DNA fragments encompassing the ITS region and the D1-D2 domains of the 28S gene were amplified using the following primer combinations: ITS5/LR6, or ITS1/ITS4 and LR0R/LR6 in cases of difficult amplification (White et al. 1990;Moncalvo et al. 2000). The polymerase chain reaction (PCR) profile for primers ITS5/LR6 was as follows: touchdown PCR initiated with a 2-min denaturation at 94 C, annealing temperature for first amplification cycle 60 C, subsequently incrementally reduced by 1 C per cycle for the next 9 cycles, followed by 36 amplification cycles each consisting of 30 s denaturation at 94 C, 30 s annealing at 50 C, and 1 min extension at 72 C, concluding with 10 min incubation at 72 C. PCR regimes using other primer combinations followed the protocols of Kučera et al. (2014b). Amplicons of the domain 6-7 region of the second largest subunit of RNA polymerase II (RPB2) were obtained using primers fRPB2-5F (Liu et al. 1999) and RPB2-P7R (Hansen et al. 2005), following protocols outlined by Hansen et al. (2005). ...
... Two basic groups of green earth tongues can be distinguished easily: (i) a group characterized by scaly stipes, including M. viride, M. rickii Imai, and M. griseoviride (Kučera et al. 2014b Description of the lectotype: Ascocarps (10-)16.8-31.3 (-38) mm high, tongue-like or club-shaped, stipitate. ...
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Four new species of the Ascomycete genus Microglossum are recognized, based on morphological characters and DNA sequences of nuc rDNA (ITS region and 28S gene) and the second largest subunit of RNA polymerase II (RPB2). They differ from Microglossum nudipes by the color of the ascocarps and the sizes and shapes of ascospores, asci, and paraphyses. A lectotype is proposed, and an emended description is provided for M. nudipes. Descriptions of new species Microglossum clavatum, M. truncatum, M. pretense, and M. tenebrosum are provided. Other closely related species in the group of green earth tongues include Microglossum viride, M. rickii, and M. griseoviride. An identification key to green Microglossum species is presented.
... Lungo le sponde di uno di questi, soprattutto nella parte bassa, vicino all'acqua corrente, è stato raccolto un gran numero di sporofori di un ascomicete, poi determinato come Microglossum griseoviride V. Kučera, Lizoň & M. Tomšovský. L'importanza del ritrovamento di tale taxon risiede nel fatto che questa rappresenta la prima segnalazione su tutto il territorio nazionale, avvenuta a solo un anno di distanza dalla sua istituzione come nuova specie [Kučera et al., 2014a]; la specie morfologicamente più simile, dalla quale M. griseoviride è stato differenziato su base molecolare, è M. viride (Pers. : Fr.) Gillet. ...
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