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Budding yeasts and Gram positive cocci clusters in HVS (photo credit, Dr. Samira Afroz) Rapid and precise detection of pathogens in tissue samples or body fluids like ascitic fluid, pleural fluid, bronchoalveolar lavage, gastrointestinal lavage are vital to predict an infectious process. Important issues such as cerebrospinal fluid and culture positive finding of pathogens on Gram reaction and morphology serve as best tool in rapid diagnosis (5). In association with other biochemical tests, Gram staining is considered as a cornerstone of a clinical laboratory for decades (6). It yields result much faster than culture and provides important data for the patient's treatment and diagnosis. Swiftness in Blood culture positive cases especially from NICU or ICU, contributes important guidance towards commencement
Source publication
not available Bangladesh J Med Microbiol 2017; 11 (2): 1-4
Citations
Chemically manipulating bacterial surface structures, a cutting‐edge research direction in the biomedical field, predominantly relies on metabolic labeling by now. However, this method may involve daunting precursor synthesis and only labels nascent surface structures. Here, we report a facile and rapid modification strategy based on a tyrosinase‐catalyzed oxidative coupling reaction (TyOCR) for bacterial surface engineering. This strategy employs phenol‐tagged small molecules and tyrosinase to initiate direct chemical modification of Gram‐positive bacterial cell walls with high labeling efficiency, while Gram‐negative bacteria are inert to this modification due to the hindrance of an outer membrane. By using the biotin‒avidin system, we further present the selective deposition of various materials, including photosensitizer, magnetic nanoparticle, and horseradish peroxidase, on Gram‐positive bacterial surfaces, and realize the purification/isolation/enrichment and naked‐eye detection of bacterial strains. This work demonstrates that TyOCR is a promising strategy for engineering live bacterial cells. This work describes a mild and efficient enzyme‐catalyzed chemical reaction for the selective modification of Gram‐positive bacterial cell walls. The as‐designed technique can serve as a universal platform to functionalize the surfaces of live bacterial cells with diverse responsive materials, including small‐molecule fluorescent dyes, horseradish peroxidase, and magnetic nanoparticles.
