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Boundary positions after 1 h of the beginning of observations of sedimentation of blood of a healthy donor (p < 0.05)
Source publication
Dependence of dynamics of blood sedimentation upon successive dilution of blood with saline solution in vitro was studied basing on the application of the principles of the system of technical vision. Oscillatory nature of the process of sedimentation of boundary between blood cells and plasma dependent on the degree of blood dilution with saline w...
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Introduction: The aim of the current study was to investigate the effects of X-ray radiation on some hematological parameters, morphology of red blood cells (RBC) and erythrocyte sedimentation rate (ESR) analysis of human blood using computed tomography (CT) scanner.
Material and Methods: For the purpose of the study, 5 ml of wholeblood was drawn f...
Citations
italic xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">Background,
cold plasma has some unique properties that enabled it to achieve its desired goals in blood coagulations. However, the coagulation mechanism activated by cold plasma remains elucidated.
Aims
, this study aims at revealing the mechanism of blood coagulations stimulated by cold plasma.
Methods,
different blood samples with different anticoagulant as well as without anticoagulant were collected from healthy volunteers. Whole blood samples were divided into two groups: 1) control group and 2) group was exposed to cold plasma until clotting. Clotting factors including fibrinogen, prothrombin time (PT) and partial thromboplastin time (PTT) as well as full blood count were analyzed for each sample. Scanning electron microscopic and light microscopic analyses were performed for platelets and red blood cells (RBCs), respectively. Also, zeta potential was measured for all blood as well as plasma-activated water (PAW) samples. Additionally, particles size was measured for PAW samples.
Results,
all blood samples with different anti-coagulant, as well as serum were coagulated after exposure to cold plasma. Moreover, the levels of clotting factors and blood cells’ count show no significant differences before and after plasma exposure. Interestingly, the morphology of both RBCs and platelets were not affected by cold plasma exposure. Meanwhile, there were a highly-significant reduction in zeta potential of blood samples; 96.4% (from
$-$
2.91 to
$-$
0.105 mV) after plasma exposure. In addition, there were highly significant increases in particles size of PAW as compared with the control sample. In conclusion, for the first time the current study revealing that, blood coagulation by cold plasma is reversible synthetic blood coagulation and neither the clotting factors nor the platelets are the main players during blood coagulation by cold plasma.
The dependence of the sedimentation rate of donor blood on its percentage in the sample (the degree of its dilution with physiologic saline) ranging from 0.25 to 100% (whole undiluted blood) is investigated with digital video equipment. The ratio of the value of displacement of the sediment–supernatant boundary per time unit was taken as the blood sedimentation rate. In case of registration of different speeds of movement of this boundary in a given volume of the cuvette, their spatial averaging was performed. A substantial increase in the blood sedimentation rate was found experimentally at high levels of its dilution, and, conversely, at blood concentrations in physiologic saline solutions that are close to whole blood. At the same time, the rate of blood sedimentation decreased several times with blood content in the range of 30–50%. It was experimentally shown that variation of blood content in the physiologic saline solution over a wide range did not affect the evenness of sedimentation occurring for almost any blood concentration in the mixture. In other words, in the process of sedimentation of a single blood sample of a given dilution in the physiologic saline solution, the sedimentation rate does not change with time. The experimentally obtained results allowed to build a theoretical model of blood sedimentation for two cases: 1) sedimentation of free erythrocytes (highly diluted blood) and 2) sedimentation of erythrocyte aggregates (almost undiluted blood, up to whole blood). The originality of the model comes from the proposition to consider the sedimentation of cells and/or their aggregates as a collective effect, and not in the traditional form of sedimentation of individual particles or particles interacting with each other. This model gives a satisfactory agreement with experimental laws with the use of certain empirical constants. The study is useful for understanding the process of erythrocyte sedimentation.
Rapid Electrokinetic Patterning (REP) is a technique for creating Self Assembled Monolayers (SAMs) of spherical particles in a liquid medium, and dynamically controlling them though the simultaneous application of an electric field and optically-induced temperature gradients. Previous work has investigated and characterized REP axisymmetric aggregations generated from a focus laser within a uniform electric field; work herein characterizes line-shaped particle assemblies derived from the application of a linearly-scanned laser. The resulting aggregations of spherical polystyrene particles (1 μm) suspended in low-conductivity aqueous potassium chloride solution (KCl, 2.5 mS/m) resembled elliptical-shaped crystalline geometries. The mean particle-to-particle spacing within the aggregation remained greater than 1.5 diameters for experiments herein (6.5 Vrms , 30 kHz) due to dipole-dipole repulsive forces. Interparticle spacing demonstrated a linear relationship (1.6 μm to 2.1 μm) with increasing scanning lengths (up to 83 μm), decreased from 1.9 μm to 1.7 μm with increasing scanning frequency (0.38 Hz to 16 Hz) for a 53 μm scan length, and decreased from 2.0 μm to 1.6 μm with increasing laser power (11.9 mW to 18.8 mW) for a 59 μm, 16 Hz laser scan. This article is protected by copyright. All rights reserved.
This article is protected by copyright. All rights reserved.
