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Biomass and lipid production for the 6 cycles (I−VI) of a repeated-batch process of C. cryptica under the heterotrophic conditions described in the legend of Figure 1 over 42 days. (A) Biomass production, expressed as g L −1 culture; (B) lipid content, expressed as % of Dry Cell Weight (DCW); (C) triglyceride (TAG) content expressed as percentage of total glycerolipids. Data are referred to the final point of each cycle after 7 days of cultivation per cycle. Data are expressed as mean ± SD, n = 6.

Biomass and lipid production for the 6 cycles (I−VI) of a repeated-batch process of C. cryptica under the heterotrophic conditions described in the legend of Figure 1 over 42 days. (A) Biomass production, expressed as g L −1 culture; (B) lipid content, expressed as % of Dry Cell Weight (DCW); (C) triglyceride (TAG) content expressed as percentage of total glycerolipids. Data are referred to the final point of each cycle after 7 days of cultivation per cycle. Data are expressed as mean ± SD, n = 6.

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Despite the theoretical high productivity, microalgae-based oil production is not economically sustainable due to the high cost of photoautotrophic cultures. Heterotrophic growth is a suitable economic alternative to overcoming light dependence and climatic/geographic fluctuations. Here we report data about growth performance, biomass production, a...

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Context 1
... whole process was repeated for 6 cycles in 42 days. During this time frame, biomass production ranged from 1.7 to 1.4 g L −1 with a median value of 1.5 ± 0.1 g L −1 ( Figure 4A). Considering biomass production, sugar-tobiomass conversion was between 0.37 and 0.45, with a median value of 0.4. ...
Context 2
... biomass production, sugar-tobiomass conversion was between 0.37 and 0.45, with a median value of 0.4. Lipid content, indicated as % of dry cell weight, varied in a range from 16 to 21%, with a median value of 18% ( Figure 4B). ...
Context 3
... composition was not significantly affected by the repeated-batch production (not shown), thus supporting the stability of C. cryptica lipid metabolism under heterotrophic conditions. TAG was the most abundant pool and represented 54−65% of total glycerolipids ( Figure 4C), with an average yield of 0.11 g TAG/g biomass. GL remained below 20%, whereas PL showed a higher variability ranging from 16 to 26% ( Figure S4). ...
Context 4
... was the most abundant pool and represented 54−65% of total glycerolipids ( Figure 4C), with an average yield of 0.11 g TAG/g biomass. GL remained below 20%, whereas PL showed a higher variability ranging from 16 to 26% ( Figure S4). Negligible levels of FFA were detected in the lipid extracts. ...