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Best scoring RAxML tree based on a combined dataset of ITS, SSU and LSU. RAxML bootstrap support values (equal or greater than 50 %) and the Bayesian posterior probabilities (equal or greater than 0.9) and are given at the nodes (ML/PP). The tree was rooted to Pleospora herbarum (CBS 191.86). All sequences from ex-type strains are in bold. Newly generated sequences are shown in green.
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Didymosphaeria spartii was collected from dead branches of Spartium junceum in Italy. Multi-gene phylogenetic analyses of ITS, 18S and 28S nrDNA sequence data were carried out using maximum likelihood and Bayesian analysis. The resulting phylogenetic trees showed this to be a new genus in a well-supported clade in Massarinaceae. A new genus Pseudod...
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... Frame Work and images used for figures were processed with Adobe Photoshop CS3 Extended version 10.0 software. Isolates were derived via single spore isolation following the method of Chomnunti et al. (2014). Ascospore germination were examined after 24 h and germinating spores were transferred to potato dextrose agar (PDA) media. The obtained pure culture was incubated at 25°C in the normal light and the cultural characteristics such as mycelium colour, shape, texture and growth rate were determined. The herbarium specimens of the new genus are deposited in the Mae Fah Luang University Herbarium (MFLU) and New Zealand Fungal & Plant Disease Collection (PDD), while cultures are deposited at the Mae Fah Luang University Culture Collection (MFLUCC) and CBS Netherlands. Fresh fungal mycelium was grown on PDA at 25°C for 21 days. Extraction of genomic DNA from mycelia was carried out following a modified method of Thambugala et al. (2015). Polymerase chain reaction (PCR) was carried out using three partial gene portions in this study. Polymerase chain reaction (PCR) was performed for DNA amplification using the primer combination LROR and LR5 (Vilgalys and Hester, 1990) for the nuclear ribosomal large subunit (LSU); NS1 and NS4 (White et al. 1990) for the nuclear ribosomal small subunit (SSU); ITS4 and ITS5 (White et al. 1990) for the internal transcribed spacer (ITS). The amplifications were performed in 25 μL of PCR mixtures containing 9.5 μL ddH2O, 12.5 μL 2×PCR Master Mix (TIANGEN Co., China), 1 μL of DNA template, 1 μL of each primer (10 μM). Conditions of amplification for all regions were consisted an initial denaturation step of 5 min at 94 °C and final elongation step of 10 minutes at 72 °C. For the SSU and LSU amplification, the 37 cycles consisted of denaturation at 94°C for 1 minute, annealing at 54°C for 50 seconds and elongation at 72°C for 1 minute; for the ITS amplification the 34 cycles consisted of denaturation at 94°C for 30 seconds, annealing at 55°C for 30 seconds and elongation at 72°C for 1 minute. The PCR products were observed on 1% agarose electrophoresis gels stained with Ethidium bromide. Purification and sequencing of PCR products were carried at using the abovementioned PCR primer at Invitrogen Biotechnology Co., China. Sequences generated from LSU, SSU and ITS were identified by BLAST analysis in the GenBank database at the National Centre for Biotechnology Information (NCBI) and sequences were analyzed with other sequences obtained from GenBank (Table 1). Most reliable sequences for taxa in Massarinaceae and representative taxa in Didymosphaeriaceae and Lentitheciaceae were included (Ariyawansa et al. 2014a; Hyde et al. 2013; Zhang et al. 2012). Pleospora herbarum (CBS 191.86) was selected as the out group taxon. The sequence data were aligned and combined using Bioedit (Hall 1999) and MEGA 5.0 (Tamura et al. 2011) and refined visually. The phylogenetic analysis consisted of two methods: The maximum likelihood analysis was performed at the CIPRES webportal using RAxML v.7.2.8 as part of the “RAxML-HPC2 on TG” tool (Stamatakis et al. 2008). The general time reversible model (GTR) using proportion of invariable sites was applied with a discrete gamma distribution and four rate classes. The best scoring tree was selected with a final likelihood value of -8232.350286. Bayesian analysis was performed using MrBayes v. 3.0b4 (Ronquist and Huelsenbeck 2003). The nucleotide substitution models were determined with MrModeltest v. 2.2 (Nylander 2004). Posterior probabilities (PP) (Rannala and Yang 1996; Zhaxybayeva and Gogarten 2002) were defined by Bayesian Markov Chain Monte Carlo (BMCMC) sampling method in MrBayes v. 3.0b4 (Huelsenbeck and Ronquist 2001). Six simultaneous Markov chains were run for 1000000 generations and trees were sampled every 100 th generation resulting in 10000 total trees. 8000 trees were used for calculating posterior probabilities (PP) in the majority rule consensus tree, after discarding the first 2000 trees representing the burn-in phase (20 %) of the analysis. The resulting trees were visualized with TreeView v. 1.6.6 (Page, 1996). The Bayesian posterior probabilities (PP- equal or greater than 0.9) and RAxML bootstrap support values (ML- equal or greater than 50%) are given at the nodes. The sequences generated in this study were deposited in GenBank. Twenty-eight taxa were included in the combined LSU, SSU and ITS data with Pleospora herbarum (CBS 191.86) as the outgroup taxon. Tree topology of the Bayesian analysis (not shown) was almost compatible with the ML tree and the best scoring RAxML tree with a final likelihood value of -8829.280968 is shown in Figure 1. The taxa belonging to families Didymosphaeriaceae, Massarinaceae and Lentitheciaceae separated into three distinct clades. Taxa in Massarinaceae formed five distinct clades. The clades Massarina , Pseudodidymosphaeria and Stagonospora are well-supported and resolved in the phylogenetic tree (Figure 1). Corynespora leucadendri (CBS 135133) clustered in a separate clade, while Byssothecium circinans (CBS 675.92) and Corynespora olivacea (CBS 114450) formed an indistinct sister clade to the Massarina clade. These two clades remain unresolved and need more strains in order to resolve their phylogenetic placement in Massarinaceae. Neottiosporina paspali (CBS 331.37) clustered in Stagonospora clade and may not belong in Neottiosporina but in Stagonospora . Montagnula spartii (CBS 183.58) and our strains (MFLUCC 13–0273 and MFLUCC 14–1212) clustered together and formed a well-supported clade in Massarinaceae (Fig. 1). A new genus Pseudodidymosphaeria is therefore introduced to accommodate D . spartii (= Sphaeria spartii ...
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... a Nikon ECLIPSE 80i compound microscope with a Canon EOS 550D digital camera. Observations and photographs were made from material mounted in water and Indian ink was added to water mounts to show the presence of gelatinous sheaths around the ascospores. Measurements were made with the Tarosoft (R) Image Frame Work and images used for figures were processed with Adobe Photoshop CS3 Extended version 10.0 software. Isolates were derived via single spore isolation following the method of Chomnunti et al. (2014). Ascospore germination were examined after 24 h and germinating spores were transferred to potato dextrose agar (PDA) media. The obtained pure culture was incubated at 25°C in the normal light and the cultural characteristics such as mycelium colour, shape, texture and growth rate were determined. The herbarium specimens of the new genus are deposited in the Mae Fah Luang University Herbarium (MFLU) and New Zealand Fungal & Plant Disease Collection (PDD), while cultures are deposited at the Mae Fah Luang University Culture Collection (MFLUCC) and CBS Netherlands. Fresh fungal mycelium was grown on PDA at 25°C for 21 days. Extraction of genomic DNA from mycelia was carried out following a modified method of Thambugala et al. (2015). Polymerase chain reaction (PCR) was carried out using three partial gene portions in this study. Polymerase chain reaction (PCR) was performed for DNA amplification using the primer combination LROR and LR5 (Vilgalys and Hester, 1990) for the nuclear ribosomal large subunit (LSU); NS1 and NS4 (White et al. 1990) for the nuclear ribosomal small subunit (SSU); ITS4 and ITS5 (White et al. 1990) for the internal transcribed spacer (ITS). The amplifications were performed in 25 μL of PCR mixtures containing 9.5 μL ddH2O, 12.5 μL 2×PCR Master Mix (TIANGEN Co., China), 1 μL of DNA template, 1 μL of each primer (10 μM). Conditions of amplification for all regions were consisted an initial denaturation step of 5 min at 94 °C and final elongation step of 10 minutes at 72 °C. For the SSU and LSU amplification, the 37 cycles consisted of denaturation at 94°C for 1 minute, annealing at 54°C for 50 seconds and elongation at 72°C for 1 minute; for the ITS amplification the 34 cycles consisted of denaturation at 94°C for 30 seconds, annealing at 55°C for 30 seconds and elongation at 72°C for 1 minute. The PCR products were observed on 1% agarose electrophoresis gels stained with Ethidium bromide. Purification and sequencing of PCR products were carried at using the abovementioned PCR primer at Invitrogen Biotechnology Co., China. Sequences generated from LSU, SSU and ITS were identified by BLAST analysis in the GenBank database at the National Centre for Biotechnology Information (NCBI) and sequences were analyzed with other sequences obtained from GenBank (Table 1). Most reliable sequences for taxa in Massarinaceae and representative taxa in Didymosphaeriaceae and Lentitheciaceae were included (Ariyawansa et al. 2014a; Hyde et al. 2013; Zhang et al. 2012). Pleospora herbarum (CBS 191.86) was selected as the out group taxon. The sequence data were aligned and combined using Bioedit (Hall 1999) and MEGA 5.0 (Tamura et al. 2011) and refined visually. The phylogenetic analysis consisted of two methods: The maximum likelihood analysis was performed at the CIPRES webportal using RAxML v.7.2.8 as part of the “RAxML-HPC2 on TG” tool (Stamatakis et al. 2008). The general time reversible model (GTR) using proportion of invariable sites was applied with a discrete gamma distribution and four rate classes. The best scoring tree was selected with a final likelihood value of -8232.350286. Bayesian analysis was performed using MrBayes v. 3.0b4 (Ronquist and Huelsenbeck 2003). The nucleotide substitution models were determined with MrModeltest v. 2.2 (Nylander 2004). Posterior probabilities (PP) (Rannala and Yang 1996; Zhaxybayeva and Gogarten 2002) were defined by Bayesian Markov Chain Monte Carlo (BMCMC) sampling method in MrBayes v. 3.0b4 (Huelsenbeck and Ronquist 2001). Six simultaneous Markov chains were run for 1000000 generations and trees were sampled every 100 th generation resulting in 10000 total trees. 8000 trees were used for calculating posterior probabilities (PP) in the majority rule consensus tree, after discarding the first 2000 trees representing the burn-in phase (20 %) of the analysis. The resulting trees were visualized with TreeView v. 1.6.6 (Page, 1996). The Bayesian posterior probabilities (PP- equal or greater than 0.9) and RAxML bootstrap support values (ML- equal or greater than 50%) are given at the nodes. The sequences generated in this study were deposited in GenBank. Twenty-eight taxa were included in the combined LSU, SSU and ITS data with Pleospora herbarum (CBS 191.86) as the outgroup taxon. Tree topology of the Bayesian analysis (not shown) was almost compatible with the ML tree and the best scoring RAxML tree with a final likelihood value of -8829.280968 is shown in Figure 1. The taxa belonging to families Didymosphaeriaceae, Massarinaceae and Lentitheciaceae separated into three distinct clades. Taxa in Massarinaceae formed five distinct clades. The clades Massarina , Pseudodidymosphaeria and Stagonospora are well-supported and resolved in the phylogenetic tree (Figure 1). Corynespora leucadendri (CBS 135133) clustered in a separate clade, while Byssothecium circinans (CBS 675.92) and Corynespora olivacea (CBS 114450) formed an indistinct sister clade to the Massarina clade. These two clades remain unresolved and need more strains in order to resolve their phylogenetic placement in Massarinaceae. Neottiosporina paspali (CBS 331.37) clustered in Stagonospora clade and may not belong in Neottiosporina but in Stagonospora . Montagnula spartii (CBS 183.58) and our strains (MFLUCC 13–0273 and MFLUCC 14–1212) clustered together and formed a well-supported clade in Massarinaceae (Fig. 1). A new genus Pseudodidymosphaeria is therefore introduced to accommodate D . spartii (= Sphaeria spartii ...
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... Fungal & Plant Disease Collection (PDD), while cultures are deposited at the Mae Fah Luang University Culture Collection (MFLUCC) and CBS Netherlands. Fresh fungal mycelium was grown on PDA at 25°C for 21 days. Extraction of genomic DNA from mycelia was carried out following a modified method of Thambugala et al. (2015). Polymerase chain reaction (PCR) was carried out using three partial gene portions in this study. Polymerase chain reaction (PCR) was performed for DNA amplification using the primer combination LROR and LR5 (Vilgalys and Hester, 1990) for the nuclear ribosomal large subunit (LSU); NS1 and NS4 (White et al. 1990) for the nuclear ribosomal small subunit (SSU); ITS4 and ITS5 (White et al. 1990) for the internal transcribed spacer (ITS). The amplifications were performed in 25 μL of PCR mixtures containing 9.5 μL ddH2O, 12.5 μL 2×PCR Master Mix (TIANGEN Co., China), 1 μL of DNA template, 1 μL of each primer (10 μM). Conditions of amplification for all regions were consisted an initial denaturation step of 5 min at 94 °C and final elongation step of 10 minutes at 72 °C. For the SSU and LSU amplification, the 37 cycles consisted of denaturation at 94°C for 1 minute, annealing at 54°C for 50 seconds and elongation at 72°C for 1 minute; for the ITS amplification the 34 cycles consisted of denaturation at 94°C for 30 seconds, annealing at 55°C for 30 seconds and elongation at 72°C for 1 minute. The PCR products were observed on 1% agarose electrophoresis gels stained with Ethidium bromide. Purification and sequencing of PCR products were carried at using the abovementioned PCR primer at Invitrogen Biotechnology Co., China. Sequences generated from LSU, SSU and ITS were identified by BLAST analysis in the GenBank database at the National Centre for Biotechnology Information (NCBI) and sequences were analyzed with other sequences obtained from GenBank (Table 1). Most reliable sequences for taxa in Massarinaceae and representative taxa in Didymosphaeriaceae and Lentitheciaceae were included (Ariyawansa et al. 2014a; Hyde et al. 2013; Zhang et al. 2012). Pleospora herbarum (CBS 191.86) was selected as the out group taxon. The sequence data were aligned and combined using Bioedit (Hall 1999) and MEGA 5.0 (Tamura et al. 2011) and refined visually. The phylogenetic analysis consisted of two methods: The maximum likelihood analysis was performed at the CIPRES webportal using RAxML v.7.2.8 as part of the “RAxML-HPC2 on TG” tool (Stamatakis et al. 2008). The general time reversible model (GTR) using proportion of invariable sites was applied with a discrete gamma distribution and four rate classes. The best scoring tree was selected with a final likelihood value of -8232.350286. Bayesian analysis was performed using MrBayes v. 3.0b4 (Ronquist and Huelsenbeck 2003). The nucleotide substitution models were determined with MrModeltest v. 2.2 (Nylander 2004). Posterior probabilities (PP) (Rannala and Yang 1996; Zhaxybayeva and Gogarten 2002) were defined by Bayesian Markov Chain Monte Carlo (BMCMC) sampling method in MrBayes v. 3.0b4 (Huelsenbeck and Ronquist 2001). Six simultaneous Markov chains were run for 1000000 generations and trees were sampled every 100 th generation resulting in 10000 total trees. 8000 trees were used for calculating posterior probabilities (PP) in the majority rule consensus tree, after discarding the first 2000 trees representing the burn-in phase (20 %) of the analysis. The resulting trees were visualized with TreeView v. 1.6.6 (Page, 1996). The Bayesian posterior probabilities (PP- equal or greater than 0.9) and RAxML bootstrap support values (ML- equal or greater than 50%) are given at the nodes. The sequences generated in this study were deposited in GenBank. Twenty-eight taxa were included in the combined LSU, SSU and ITS data with Pleospora herbarum (CBS 191.86) as the outgroup taxon. Tree topology of the Bayesian analysis (not shown) was almost compatible with the ML tree and the best scoring RAxML tree with a final likelihood value of -8829.280968 is shown in Figure 1. The taxa belonging to families Didymosphaeriaceae, Massarinaceae and Lentitheciaceae separated into three distinct clades. Taxa in Massarinaceae formed five distinct clades. The clades Massarina , Pseudodidymosphaeria and Stagonospora are well-supported and resolved in the phylogenetic tree (Figure 1). Corynespora leucadendri (CBS 135133) clustered in a separate clade, while Byssothecium circinans (CBS 675.92) and Corynespora olivacea (CBS 114450) formed an indistinct sister clade to the Massarina clade. These two clades remain unresolved and need more strains in order to resolve their phylogenetic placement in Massarinaceae. Neottiosporina paspali (CBS 331.37) clustered in Stagonospora clade and may not belong in Neottiosporina but in Stagonospora . Montagnula spartii (CBS 183.58) and our strains (MFLUCC 13–0273 and MFLUCC 14–1212) clustered together and formed a well-supported clade in Massarinaceae (Fig. 1). A new genus Pseudodidymosphaeria is therefore introduced to accommodate D . spartii (= Sphaeria spartii ...
Citations
... Following its introduction, many studies have been conducted on the above mentioned genera, with the exception of Massarina, have been transferred to other families (Suetrong et al. 2009, Zhang et al. 2012, Hyde et al. 2013, Wijayawardene et al. 2014). Massarina has also been placed within Lophiostomataceae in Pleosporales (Bose 1961, Barr 1992, Aptroot 1998, Thambugala et al. 2015). Massarinaceae was considered as a synonym of Lophiostomataceae in some subsequent studies (Barr 1987). ...
... Massarinaceae was considered as a synonym of Lophiostomataceae in some subsequent studies (Barr 1987). However, recent morphological and molecular studies provide evidence that these two families evolved separately (Thambugala et al. 2015). Hence, Massarinaceae and Lophiostomataceae were treated as separate families in the order Pleosporales (Liew et al. 2002, Zhang et al. 2009a, 2012, Hyde et al. 2013, Wijayawardene et al. 2014). ...
In this paper we introduce a new genus Pseudosplanchnonema with P. phorcioides comb. nov., isolated from dead branches of Acer campestre and Morus species. The new genus is confirmed based on morphology and phylogenetic analyses of sequence data. Phylogenetic analyses based on combined LSU and SSU sequence data showed that P. phorcioides formed a distinct clade within the family Massarinaceae and is sister to Massarina eburnea, the type species of Massarina. The new genus Pseudosplanchnonema differs from Massarina in having ascomata without clypei, a thick peridium and larger, 1-septate, guttulate, dark brown ascospores. The new genus is compared with genera in the family Massarinaceae and a detailed description and illustrations of the species P. phorcioides, including its asexual morph, is provided.
This paper provides an updated classification of the Kingdom Fungi (including fossil fungi)
and fungus-like taxa. Five-hundred and twenty-three (535) notes are provided for newly introduced
taxa and for changes that have been made since the previous outline. In the discussion, the latest
taxonomic changes in Basidiomycota are provided and the classification of Mycosphaerellales are
broadly discussed. Genera listed in Mycosphaerellaceae have been confirmed by DNA sequence
analyses, while doubtful genera (DNA sequences being unavailable but traditionally
accommodated in Mycosphaerellaceae) are listed in the discussion. Problematic genera in
Glomeromycota are also discussed based on phylogenetic results.
This paper provides an updated classification of the Kingdom Fungi (including fossil fungi) and fungus-like taxa. Five-hundred and twenty-three (535) notes are provided for newly introduced taxa and for changes that have been made since the previous outline. In the discussion, the latest taxonomic changes in Basidiomycota are provided and the classification of Mycosphaerellales are broadly discussed. Genera listed in Mycosphaerellaceae have been confirmed by DNA sequence analyses, while doubtful genera (DNA sequences being unavailable but traditionally accommodated in Mycosphaerellaceae) are listed in the discussion. Problematic genera in Glomeromycota are also discussed based on phylogenetic results.
This is a continuity of a series of taxonomic and phylogenetic papers on the fungi where materials were collected from many countries, examined and described. In addition to extensive morphological descriptions and appropriate asexual and sexual connections, DNA sequence data are also analysed from concatenated datasets to infer phylogenetic relationships and substantiate systematic positions of taxa within appropriate ranks. Wherever new species or combinations are proposed, we apply an integrative approach using morphological and molecular data as well as ecological features wherever applicable. Notes on 112 fungal taxa are compiled in this paper including Biatriosporaceae and Roussoellaceae, Didysimulans gen. nov., 81 new species, 18 new host records and new country records, five reference specimens, two new combinations, and three sexual and asexual morph reports. The new species are Amanita cornelii, A. emodotrygon, Angustimassarina alni, A. arezzoensis, A. italica, A. lonicerae, A. premilcurensis, Ascochyta italica, A. rosae, Austroboletus appendiculatus, Barriopsis thailandica, Berkleasmium ariense, Calophoma petasitis, Camarosporium laburnicola, C. moricola, C. grisea, C. ossea, C. paraincrustata, Colletotrichum sambucicola, Coprinopsis cerkezii, Cytospora gelida, Dacrymyces chiangraiensis, Didysimulans italica, D. mezzanensis, Entodesmium italica, Entoloma magnum, Evlachovaea indica, Exophiala italica, Favolus gracilisporus, Femsjonia monospora, Fomitopsis flabellata, F. roseoalba, Gongronella brasiliensis, Helvella crispoides, Hermatomyces chiangmaiensis, H. chromolaenae, Hysterium centramurum, Inflatispora caryotae, Inocybe brunneosquamulosa, I. luteobrunnea, I. rubrobrunnea, Keissleriella cirsii, Lepiota cylindrocystidia, L. flavocarpa, L. maerimensis, Lophiotrema guttulata, Marasmius luculentus, Morenoina calamicola, Moelleriella thanathonensis, Mucor stercorarius, Myrmecridium fluviae, Myrothecium septentrionale, Neosetophoma garethjonesii, Nigrograna cangshanensis, Nodulosphaeria guttulatum, N. multiseptata, N. sambuci, Panus subfasciatus, Paraleptosphaeria padi, Paraphaeosphaeria viciae, Parathyridaria robiniae, Penicillium punicae, Phaeosphaeria calamicola, Phaeosphaeriopsis yuccae, Pleurophoma italica, Polyporus brevibasidiosus, P. koreanus, P. orientivarius, P. parvovarius, P. subdictyopus, P. ulleungus, Pseudoasteromassaria spadicea, Rosellinia mearnsii, Rubroboletus demonensis, Russula yanheensis, Sigarispora muriformis, Sillia italica, Stagonosporopsis ailanthicola, Strobilomyces longistipitatus, Subplenodomus galicola and Wolfiporia pseudococos. The new combinations are Melanomma populina and Rubroboletus eastwoodiae. The reference specimens are Cookeina tricholoma, Gnomoniopsis sanguisorbae, Helvella costifera, Polythrincium trifolii and Russula virescens. The new host records and country records are Ascochyta medicaginicola, Boletellus emodensis, Cyptotrama asprata, Cytospora ceratosperma, Favolaschia auriscalpium, F. manipularis, Hysterobrevium mori, Lentinus sajor-caju, L. squarrosulus, L. velutinus, Leucocoprinus cretaceus, Lophiotrema vagabundum, Nothophoma quercina, Platystomum rosae, Pseudodidymosphaeria phlei, Tremella fuciformis, Truncatella spartii and Vaginatispora appendiculata and three sexual and asexual morphs are Aposphaeria corallinolutea, Dothiora buxi and Hypocrella calendulina.
This is a continuity of a series of taxonomic papers where materials are examined, described and novel combinations are proposed where necessary to improve our traditional species concepts and provide updates on their classification. In addition to extensive morphological descriptions and appropriate asexual and sexual connections, DNA sequence data are also analysed from concatenated datasets (rDNA, TEF-α, RBP2 and β-Tubulin) to infer phylogenetic relationships and substantiate systematic position of taxa within appropriate ranks. Wherever new species or combinations are being proposed, we apply an integrative approach (morphological and molecular data as well as ecological features wherever applicable). Notes on 125 fungal taxa are compiled in this paper, including eight new genera, 101 new species, two new combinations, one neotype, four reference specimens, new host or distribution records for eight species and one alternative morphs. The new genera introduced in this paper are Alloarthopyrenia, Arundellina, Camarosporioides, Neomassaria, Neomassarina, Neotruncatella, Paracapsulospora and Pseudophaeosphaeria. The new species are Alfaria spartii, Alloarthopyrenia italica, Anthostomella ravenna, An. thailandica, Arthrinium paraphaeospermum, Arundellina typhae, Aspergillus koreanus, Asterina cynometrae, Bertiella ellipsoidea, Blastophorum aquaticum, Cainia globosa, Camarosporioides phragmitis, Ceramothyrium menglunense, Chaetosphaeronema achilleae, Chlamydotubeufia helicospora, Ciliochorella phanericola, Clavulinopsis aurantiaca, Colletotrichum insertae, Comoclathris italica, Coronophora myricoides, Cortinarius fulvescentoideus, Co. nymphatus, Co. pseudobulliardioides, Co. tenuifulvescens, Cunninghamella gigacellularis, Cyathus pyristriatus, Cytospora cotini, Dematiopleospora alliariae, De. cirsii, Diaporthe aseana, Di. garethjonesii, Distoseptispora multiseptata, Dis. tectonae, Dis. tectonigena, Dothiora buxi, Emericellopsis persica, Gloniopsis calami, Helicoma guttulatum, Helvella floriforma, H. oblongispora, Hermatomyces subiculosa, Juncaceicola italica, Lactarius dirkii, Lentithecium unicellulare, Le. voraginesporum, Leptosphaeria cirsii, Leptosphaeria irregularis, Leptospora galii, Le. thailandica, Lindgomyces pseudomadisonensis, Lophiotrema bambusae, Lo. fallopiae, Meliola citri-maximae, Minimelanolocus submersus, Montagnula cirsii, Mortierella fluviae, Muriphaeosphaeria ambrosiae, Neodidymelliopsis ranunculi, Neomassaria fabacearum, Neomassarina thailandica, Neomicrosphaeropsis cytisi, Neo. cytisinus, Neo. minima, Neopestalotiopsis cocoës, Neopestalotiopsis musae, Neoroussoella lenispora, Neotorula submersa, Neotruncatella endophytica, Nodulosphaeria italica, Occultibambusa aquatica, Oc. chiangraiensis, Ophiocordyceps hemisphaerica, Op. lacrimoidis, Paracapsulospora metroxyli, Pestalotiopsis sequoiae, Peziza fruticosa, Pleurotrema thailandica, Poaceicola arundinis, Polyporus mangshanensis, Pseudocoleophoma typhicola, Pseudodictyosporium thailandica, Pseudophaeosphaeria rubi, Purpureocillium sodanum, Ramariopsis atlantica, Rhodocybe griseoaurantia, Rh. indica, Rh. luteobrunnea, Russula indoalba, Ru. pseudoamoenicolor, Sporidesmium aquaticivaginatum, Sp. olivaceoconidium, Sp. pyriformatum, Stagonospora forlicesenensis, Stagonosporopsis centaureae, Terriera thailandica, Tremateia arundicola, Tr. guiyangensis, Trichomerium bambusae, Tubeufia hyalospora, Tu. roseohelicospora and Wojnowicia italica. New combinations are given for Hermatomyces mirum and Pallidocercospora thailandica. A neotype is proposed for Cortinarius fulvescens. Reference specimens are given for Aquaphila albicans, Leptospora rubella, Platychora ulmi and Meliola pseudosasae, while new host or distribution records are provided for Diaporthe eres, Di. siamensis, Di. foeniculina, Dothiorella iranica, Do. sarmentorum, Do. vidmadera, Helvella tinta and Vaginatispora fuckelii, with full taxonomic details. An asexual state is also reported for the first time in Neoacanthostigma septoconstrictum. This paper contributes to a more comprehensive update and improved identification of many ascomycetes and basiodiomycetes.
Notes on 113 fungal taxa are compiled in this paper, including 11 new genera, 89 new species, one new subspecies, three new combinations and seven reference specimens. A wide geographic and taxonomic range of fungal taxa are detailed. In the Ascomycota the new genera Angustospora (Testudinaceae), Camporesia (Xylariaceae), Clematidis, Crassiparies (Pleosporales genera incertae sedis), Farasanispora, Longiostiolum (Pleosporales genera incertae sedis), Multilocularia (Parabambusicolaceae), Neophaeocryptopus (Dothideaceae), Parameliola (Pleosporales genera incertae sedis), and Towyspora (Lentitheciaceae) are introduced. Newly introduced species are Angustospora nilensis, Aniptodera aquibella, Annulohypoxylon albidiscum, Astrocystis thailandica, Camporesia sambuci, Clematidis italica, Colletotrichum menispermi, C. quinquefoliae, Comoclathris pimpinellae, Crassiparies quadrisporus, Cytospora salicicola, Diatrype thailandica, Dothiorella rhamni, Durotheca macrostroma, Farasanispora avicenniae, Halorosellinia rhizophorae, Humicola koreana, Hypoxylon lilloi, Kirschsteiniothelia tectonae, Lindgomyces okinawaensis, Longiostiolum tectonae, Lophiostoma pseudoarmatisporum, Moelleriella phukhiaoensis, M. pongdueatensis, Mucoharknessia anthoxanthi, Multilocularia bambusae, Multiseptospora thysanolaenae, Neophaeocryptopus cytisi, Ocellularia arachchigei, O. ratnapurensis, Ochronectria thailandica, Ophiocordyceps karstii, Parameliola acaciae, P. dimocarpi, Parastagonospora cumpignensis, Pseudodidymosphaeria phlei, Polyplosphaeria thailandica, Pseudolachnella brevifusiformis, Psiloglonium macrosporum, Rhabdodiscus albodenticulatus, Rosellinia chiangmaiensis, Saccothecium rubi, Seimatosporium pseudocornii, S. pseudorosae, Sigarispora ononidis and Towyspora aestuari. New combinations are provided for Eutiarosporella dactylidis (sexual morph described and illustrated) and Pseudocamarosporium pini. Descriptions, illustrations and / or reference specimens are designated for Aposphaeria corallinolutea, Cryptovalsa ampelina, Dothiorella vidmadera, Ophiocordyceps formosana, Petrakia echinata, Phragmoporthe conformis and Pseudocamarosporium pini. The new species of Basidiomycota are Agaricus coccyginus, A. luteofibrillosus, Amanita atrobrunnea, A. digitosa, A. gleocystidiosa, A. pyriformis, A. strobilipes, Bondarzewia tibetica, Cortinarius albosericeus, C. badioflavidus, C. dentigratus, C. duboisensis, C. fragrantissimus, C. roseobasilis, C. vinaceobrunneus, C. vinaceogrisescens, C. wahkiacus, Cyanoboletus hymenoglutinosus, Fomitiporia atlantica, F. subtilissima, Ganoderma wuzhishanensis, Inonotus shoreicola, Lactifluus armeniacus, L. ramipilosus, Leccinum indoaurantiacum, Musumecia alpina, M. sardoa, Russula amethystina subp. tengii and R. wangii are introduced. Descriptions, illustrations, notes and / or reference specimens are designated for Clarkeinda trachodes, Dentocorticium ussuricum, Galzinia longibasidia, Lentinus stuppeus and Leptocorticium tenellum. The other new genera, species new combinations are Anaeromyces robustus, Neocallimastix californiae and Piromyces finnis from Neocallimastigomycota, Phytophthora estuarina, P. rhizophorae, Salispina, S. intermedia, S. lobata and S. spinosa from Oomycota, and Absidia stercoraria, Gongronella orasabula, Mortierella calciphila, Mucor caatinguensis, M. koreanus, M. merdicola and Rhizopus koreanus in Zygomycota.
