Figure 4 - uploaded by Hae Jin Cho
Content may be subject to copyright.
Basidiomes of four new Laccaria species from Korea. A-B. L. araneosa (TPML20120912-40, holotype; SFC20130917-21). CD. L. parva (SFC20120919-40, holotype; SFC20121001-08). E-F. L. torosa (SFC20150902-17, holotype; KA12-1306). G-I. L. versiforma (SFC20120926-01, holotype; SFC20121010-51, TPML20120924-82). Bars = 10 mm.
Source publication
Species of Laccaria (Hydnangiaceae, Basidiomycota) are important in forest ecosystems as ectomycorrhizal fungi. Nine of the 75 described Laccaria species worldwide been reported from Korea. Most of these have European and North American names, and their identities are based solely on morphological features. To evaluate the taxonomy of Korean Laccar...
Citations
... In addition, L. torosa H.J. Cho & Y.W. Lim also resembles L. nanlingensis; however, L. torosa, reported from Korea, differs in having larger basidiocarps, orange-brown to brown pileus fading to pale orange buff when dry or old, and larger basidiospores (8-9×8-9.5 µm) [50]. ...
... In the past, many Chinese samples of Laccaria were inaccurately labeled as L. laccata, L. bicolor, L. amethystea and L. vinaceoavellanea [72][73][74][75]. Phylogenetic studies have made some species well-understood in China, and the discovery of new species in Laccaria is rapidly increasing [2,17,21,22,[31][32][33][34]50]. However, the distribution of L. amethystea, L. bicolor and L. laccata in China still needs to be investigated. ...
The genus Laccaria is a type of cosmopolitan and ecologically important fungal group. Members can form ectomycorrhizal associations with numerous trees, and some species are common edible fungi in local markets. Although some new species from China are recently published, the species diversity of Laccaria is still unclear in China. In this study, some samples of Laccaria were collected from southern China, and morphological characteristics and phylogenetic analyses based on the multilocus dataset of ITS-LSU-tef1-rpb2 confirmed five new species. Laccaria miniata, L. nanlingensis and L. neovinaceoavellanea were collected from subtropical broad-leaved forests, and L. rufobrunnea and L. umbilicata were collected from subtropical mixed forests of southwest China. Full descriptions, illustrations, comparisons with similar species and phylogenetic analysis are provided.
... album have persisted in the Republic of Korea for over 80 years [24]. Recent studies based on the SBC and SBI have revealed that many Asian mycorrhizal species have been misidentified, often using the names of European and American species [25][26][27]. Hydnum repandum and H. repandum var. album are ectomycorrhizal species named after European specimens; however, the applicability of these names has not been clarified through molecular analyses. ...
Hydnum is a genus of ectomycorrhizal fungi belonging to the Hydnaceae family. It is widely distributed across different regions of the world, including North America, Europe, and Asia; however, some of them showed disjunct distributions. In recent years, with the integration of molecular techniques, the taxonomy and classification of Hydnum have undergone several revisions and advancements. However, these changes have not yet been applied in the Republic of Korea. In this study, we conducted an integrated analysis combining the morphological and molecular analyses of 30 specimens collected over a period of approximately 10 years in the Republic of Korea. For molecular analysis, the sequence data of the internal transcribed spacer (ITS) region, the large subunit of nuclear ribosomal RNA gene (nrLSU), and a portion of translation elongation factor 1-α (TEF1) were employed as molecular markers. Through this study, we identified eight species that had previously not been reported to occur in the Republic of Korea, including one new species, Hydnum paucispinum. A taxonomic key and detailed descriptions of the eight Hydnum species are provided in this study.
... PCR amplicons were purified with the QIAquick Purification Kit (Qiagen, Inc.) and sequenced by Macrogen sequencing service (Macrogen Inc., Seoul, Korea). For phylogenetic study, 61 sequences were download from GenBank (Table 1) [3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21]. The dataset was aligned using MAFFT v.7, with all other parameters set to default values. ...
In 2022, our investigation focused on exploring the macrofungal diversity in South Korea. Through the examination of morphological characteristics and ITS sequences of the rDNA, we successfully identified four unrecorded species (Laccaria striatula , Leotia atrovirens, Malvipezia emileia, Xerula strigosa). Detailed morphological descriptions, accompanied by photographs and molecular phylogenetic analyses were performed.
... sororia tudung pada posisi central, tipe penempelan pada substrat berupa basal tomentum, dan tidak berongga (solid). Hingga saat ini posisi taksonomi dari Laccaria masih dinamis dan beberapa revisi terus dilakukan(Cho et al., 2018). Laccaria vinaceoavellanea merupakan spesies JPE yang sebelumnya dilaporkan tersebar pada 3 negara yakni Jepang, Laos, dan Malaysia dengan 225 catatan keberadaan (GBIF, 2021). ...
Jamur pembentuk ektomikoriza (JPE) merupakan salah satu organisme yang memiliki peran penting pada habitat terestrial. Sebagian besar dari jamur tersebut bersimbiosis dengan dengan tanaman kehutanan dan merupakan salah satu indikator kesehatan hutan. Informasi mengenai keragaman JPE di Indonesia masih terbatas dan sebagian besar tidak memiliki pertelaan tubuh buah. Penelitian ini bertujuan untuk menginventarisasi keragaman JPE di Kawasan Hutan Penelitian Haurbentes (Jawa Barat) serta menyediakan deskripsi basidiomatanya. Eksplorasi JPE dilakukan dengan metode sampling oportunistik. Hasil identifikasi menunjukkan terdapat sebanyak 11 spesies JPE yang terdiri atas 3 ordo dan 6 family. Jenis JPE tersebut adalah : Cortinarius sp., Laccaria cf. vinaceoavellanea, Inocybe sp., Scleroderma sp., Boletus sp., Phylloporus sp., Pulveroboletus sp., Lactarius cf. javanicus, Lactarius cf. corrugatus, Russula cf. cyanoxantha dan Russula cf. sororia. Beberapa jamur diketahui memiliki potensi sebagai bahan pangan (Scleroderma sp., Boletus sp., Phylloporus sp., Lactarius spp., dan Russula spp.), mikoremediator (Laccaria cf. vinaceoavellanea), dan antikanker (Pulveroboletus sp.). Hasil penelitian ini menambah informasi mengenai keragaman JPE di Indonesia. Upaya monitoring berkala terhadap jamur dan tumbuhan di area tersebut diperlukan untuk menjaga kesinambungan simbiosis yang terbentuk.
The ectomycorrhizal-forming fungi (JPE) is one of the organisms which has an important role in terrestrial habitats. Most of these fungi are in the symbiosis with forestry plants and are an indicator of forest health. Information on the diversity of JPE in Indonesia is still limited and most of them do not have a description of the fruiting body. This study aimed to inventory the diversity of JPE in the Haurbentes Research Forest Area (West Java) and provide the description of its basidiomata. JPE exploration was carried out using the opportunistic sampling method. The identification results showed that there were 11 species of JPE consisting of 3 orders and 6 families. The species of JPE are: Cortinarius sp., Laccaria cf. vinaceoavellanea, Inocybe sp., Scleroderma sp., Boletus sp., Phylloporus sp., Pulveroboletus sp., Lactarius cf. javanicus, Lactarius cf. corrugatus, Russula cf. cyanoxantha and Russula cf. sororia. Some of the JPE have the potential as food source (Scleroderma sp., Boletus sp., Phylloporus sp., Lactarius spp., and Russula spp.), mycoremediation agent (Laccaria cf. vinaceoavellanea), and anticancer (Pulveroboletus sp.).The results of this study contributeto the additional information about the diversity of JPE in Indonesia. Periodic monitoring efforts of fungi and plants in this area are needed to maintain the sustainability of the symbiosis.
... It is estimated that 116 species of Laccaria are distributed across temperate and tropical regions of the world (Wilson et al. 2017). The diversity and taxonomy of the genus Laccaria have been well documented (Mueller 1984) including with phylogenetic and molecular studies (Osmundson et al. 2005, Vincenot et al. 2012, Sheedy et al. 2013, Wilson et al. 2013, Popa et al. 2014, Montoya et al. 2015, Cho et al. 2018. More recently, biogeographic (Wilson et al. 2017) and genomic studies have used some Laccaria taxa (e.g., Martin et al. 2008, Kang et al. 2020, Li et al. 2020 as model species in the understanding of the physiology, ecology, and evolution of ectomycorrhizal symbiosis. ...
Background:
Pinus (Coniferophyta) and Laccaria (Basidiomycota) establish ectomycorrhizal symbioses in natural forests. However, their detailed morphoanatomical and phylogenetic characterization have received little attention. Accurate identification of native host symbionts is of paramount relevance to the production of mycorrhized seedlings for successful reforestation programs.
Questions/Objective:
We aimed to determine if L. squarrosa is able to establish ectomycorrhizal symbiosis with gymnosperms, thereby widening its host range and highlighting its relevance as a potential inoculant for pine seedlings. Currently, L. squarrosa is only known from its type collection associated with the angiosperm Fagus grandifolia var. mexicana.
Studied species:
The fungus L. squarrosa and Pinus pseudostrobus, a tree endemic to Mexico.
Study site and dates:
A Pinus-Quercus forest in Piedra Canteada, Nanacamilpa, Tlaxcala; 2018-2020.
Methods:
L. squarrosa basidiomata were identified and ectomycorrhizal roots were collected and morphoanatomically characterized. For molecular identification, DNA was extracted, PCR was performed targeting the nuclear ribosomal internal transcribed spacer region (nucrDNA ITS) for the mycobiont identification and the chloroplastic single-locus trnL region for the phytobiont.
Results:
In the phylogenetic analyses, our sequences from basidiomata and ectomycorrhizae clustered together with L. squarrosa with high values of supporting identity. Meanwhile, P. pseudostrobus was molecularly identified as the phytobiont.
Conclusions:
This is one of the few worldwide characterizations of Laccaria ectomycorrhiza under field conditions and contributes to the understanding of the ecology, distribution, and economic relevance of the symbiotic association. Our data suggest that L. squarrosa has potential for use as a native inoculant for P. pseudostrobus tree production.
... Notes: Laccaria currently comprises about 85 species ), but numerous new species have been described in recent years (Wilson et al. 2013(Wilson et al. , 2016Popa et al. 2014;Luo et al. 2016;Ramos et al. 2017;Vincenot et al. 2017;Cho et al. 2018;Wang et al. 2019b), a clear indication that the exact number of species in this genus is still undetermined. Laccaria species form mutualistic symbioses with many shrubs and forest tree species and are widely distributed over geographical areas from the tropics to the boreal regions (Gardes et al. 1990;Mueller 1992;Vincenot et al. 2011;Popa et al. 2014). ...
... Lamellae up to 5 mm broad, moderately distant, emarginate, sinuate to adnate with Fig. 182 Phylogram generated from maximum likelihood analysis based on LSU and ITS sequence data representing Laccaria of northern hemisphere and tropical areas. Related sequences aretaken from previous studies (Wilson et al. 2013;Popa et al. 2014;Luo et al. 2016;Ramos et al. 2017;Vincenot et al. 2017;Cho et al. 2018;Wang et al. 2019b). One hundred fifty-six strains are included in the combined analyses which comprise 1624 characters. ...
This article is the 13th contribution in the Fungal Diversity Notes series, wherein 125 taxa from four phyla, ten classes, 31 orders, 69 families, 92 genera and three genera incertae sedis are treated, demonstrating worldwide and geographic distribution. Fungal taxa described and illustrated in the present study include three new genera, 69 new species, one new combination, one reference specimen and 51 new records on new hosts and new geographical distributions. Three new genera, Cylindrotorula (Torulaceae), Scolecoleotia (Leotiales genus incertae sedis) and Xenovaginatispora (Lindomycetaceae) are introduced based on distinct phylogenetic lineages and unique morphologies. Newly described species are Aspergillus lannaensis, Cercophora dulciaquae, Cladophialophora aquatica, Coprinellus punjabensis, Cortinarius alutarius, C. mammillatus, C. quercofocculosus, Coryneum fagi, Cruentomycena uttarakhandina, Cryptocoryneum rosae, Cyathus uniperidiolus, Cylindrotorula indica, Diaporthe chamaeropicola, Didymella azollae, Diplodia alanphillipsii, Dothiora coronicola, Efbula rodriguezarmasiae, Erysiphe salicicola, Fusarium queenslandicum, Geastrum gorgonicum, G. hansagiense, Helicosporium sexualis, Helminthosporium chiangraiensis, Hongkongmyces kokensis, Hydrophilomyces hydraenae, Hygrocybe boertmannii, Hyphoderma australosetigerum, Hyphodontia yunnanensis, Khaleijomyces umikazeana, Laboulbenia divisa, Laboulbenia triarthronis, Laccaria populina, Lactarius pallidozonarius, Lepidosphaeria strobelii, Longipedicellata megafusiformis, Lophiotrema lincangensis, Marasmius benghalensis, M. jinfoshanensis, M. subtropicus, Mariannaea camelliae, Melanographium smilaxii, Microbotryum polycnemoides, Mimeomyces digitatus, Minutisphaera thailandensis, Mortierella solitaria, Mucor harpali, Nigrograna jinghongensis, Odontia huanrenensis, O. parvispina, Paraconiothyrium ajrekarii, Parafuscosporella niloticus, Phaeocytostroma yomensis, Phaeoisaria synnematicus, Phanerochaete hainanensis, Pleopunctum thailandicum, Pleurotheciella dimorphospora, Pseudochaetosphaeronema chiangraiense, Pseudodactylaria albicolonia, Rhexoacrodictys nigrospora, Russula paravioleipes, Scolecoleotia eriocamporesi, Seriascoma honghense, Synandromyces makranczyi, Thyridaria aureobrunnea, Torula lancangjiangensis, Tubeufa longihelicospora, Wicklowia fusiformispora, Xenovaginatispora phichaiensis and Xylaria apiospora. One new combination, Pseudobactrodesmium stilboideus is proposed. A reference specimen of Comoclathris permunda is designated. New host or distribution records are provided for Acrocalymma fci, Aliquandostipite khaoyaiensis, Camarosporidiella laburni, Canalisporium caribense, Chaetoscutula juniperi, Chlorophyllum demangei, C. globosum, C. hortense, Cladophialophora abundans, Dendryphion hydei, Diaporthe foeniculina, D. pseudophoenicicola, D. pyracanthae, Dictyosporium pandanicola, Dyfrolomyces distoseptatus, Ernakulamia tanakae, Eutypa favovirens, E. lata, Favolus septatus, Fusarium atrovinosum, F. clavum, Helicosporium luteosporum, Hermatomyces nabanheensis, Hermatomyces sphaericoides, Longipedicellata aquatica, Lophiostoma caudata, L. clematidisvitalbae, Lophiotrema hydei, L. neoarundinaria, Marasmiellus palmivorus, Megacapitula villosa, Micropsalliota globocystis, M. gracilis, Montagnula thailandica, Neohelicosporium irregulare, N. parisporum, Paradictyoarthrinium difractum, Phaeoisaria aquatica, Poaceascoma taiwanense, Saproamanita manicata, Spegazzinia camelliae, Submersispora variabilis, Thyronectria caudata, T. mackenziei, Tubeufa chiangmaiensis, T. roseohelicospora, Vaginatispora nypae, Wicklowia submersa, Xanthagaricus necopinatus and Xylaria haemorrhoidalis. The data presented herein are based on morphological examination of fresh specimens, coupled with analysis of phylogenetic sequence data to better integrate taxa into appropriate taxonomic ranks and infer their evolutionary relationships.
... Kearse et al. 2012) and compared to those available in the GenBank database (https://www.ncbi.nlm.nih.gov/genbank/) using the BLASTn search method. Our dataset includes sequences of Laccaria from the Northern Hemisphere and tropical regions retrieved from previous phylogenetic studies (Wilson et al. 2013, Popa et al. 2014, Luo et al. 2016, Vincenot et al. 2017, Ramos et al. 2017, Cho et al. 2018, Wang et al. 2019 (1986: 643) was used as the outgroup taxon following Luo et al. (2016). ITS and LSU sequences were independently aligned using MAFFT v 7.017 (Katoh et al. 2002) with default conditions for gap openings and gap extension penalties and subsequently concatenated. ...
Laccaria macrocystidiata, a marasmioid species, originally described as Laccaria affinis f. macrocystidiata from Central Italy, was synonymised with the North American taxon Laccaria laccata var. pallidifolia, but only on a morphological basis. In this paper, the independent position of L. macrocystidiata from L. laccata var. pallidifolia and other species of Laccaria is pointed out on the basis of ITS-LSU phylogenetic analyses. According to our results, Laccaria macrocystidiata var. longispinosa is considered a synonym of Laccaria macrocystidiata. Laccaria macrocystidiata holotype consists of only one basidioma in poor condition; thus, a new epitype from an Italian collection was designated.
... Therefore, it is virtually required to conduct molecular analyses in order to accurately identify Laccaria species. Clear demarcation of Laccaria species in phylogeny has become explicit based on the analysis of multigene sequence data, which include the nuclear rDNA internal transcribed spacer (ITS) region, partial regions of nuclear 28S rDNA (28S), RNA polymerase II subunit 2 (rpb2), and translation elongation factor 1-a (tef1) [4,5,[11][12][13]. Well-established phylogeny and curated sequence information are highly useful in discovering new species or conducting other molecular-based studies [14,15]. ...
... Classically, information on the diversity and distribution of ECM fungi is based on a survey of fungal fruiting bodies [16,17]. We have previously confirmed 13 Laccaria species based on specimens collected from fruiting bodies [12,13]. However, this approach may only partially reflect fungal diversity because many species do not produce conspicuous fruiting bodies [18][19][20][21][22]. Soil-based next-generation sequencing (NGS) techniques seem to overcome the shortcomings of the above method in studies of soil fungal communities [23][24][25]. ...
... Finally, we attempted the SSP to confirm the presence of L. nigra and L. ohiensis. These species were recorded in Korea but were not found in specimens [12,13]. We were able to design a species-specific primer set only for L. ohiensis (KH-07192006-1), as reliable L. nigra sequences could not be secured. ...
The genus Laccaria (Hydnangiaceae, Agaricales) plays an important role in forest ecosystems as an ectomycorrhizal fungus, contributing to nutrient cycles through symbiosis with many types of trees. Though understanding Laccaria diversity and distribution patterns, as well as its association with host plants, is fundamental to constructing a balanced plant diversity and conducting effective forest management, previous studies have not been effective in accurately investigating, as they relied heavily on specimen collection alone. To investigate the true diversity and distribution pattern of Laccaria species and determine their host types, we used four different approaches: specimen-based analysis, open database search (ODS), NGS analysis, and species-specific PCR (SSP). As a result, 14 Laccaria species have been confirmed in Korea. Results regarding the species distribution pattern were different between specimen-based analysis and SSP. However, when both were integrated, the exact distribution pattern of each Laccaria species was determined. In addition, the SSP revealed that many Laccaria species have a wide range of host types. This study shows that using these four different approaches is useful in determining the diversity, distribution, and host of ECM fungi. Furthermore, results obtained for Laccaria will serve as a baseline to help understand the role of ECM fungi in forest management in response to climate change.
... Laccaria is a cosmopolitan genus and many studies have contributed to its species diversity since its publication in the year of 1883 (Berkeley and Broome 1883;Heinemann 1964;Singer 1967;Besson and Kühner 1971;Kühner 1980;Lahaie 1981;Bon 1983;Clémençon 1984;Mueller 1984Mueller , 1991Mueller , 1992Singer 1986;Vellinga 1986;Ballero andContu 1987, 1989;May 1991;Kropp and Mueller 1999;Wang et al. 2004;Osmundson et al. 2005;Wilson et al. 2013Wilson et al. , 2017Popa et al. 2014Popa et al. , 2016Luo et al. 2016;Vincenot et al. 2017;Cho et al. 2018Cho et al. , 2020Corrales et al. 2020;Li 2020). Species in this genus have a strong geographical differentiation, at least in the Laccaria sect. ...
... In China, most Laccaria species were applied with western names before the study of Wang et al. (2004). Since then, several studies have documented the Chinese Laccaria species, especially from the southwestern regions (Wang et al. 2004;Wilson et al. 2013;Popa et al. 2014;Luo et al. 2016;Vincenot et al. 2017;Cho et al. 2018;Li 2020). During the past 20 years, ca. ...
... During the past 20 years, ca. 19 Laccaria species were reported from China with both molecular and morphological evidence (Wang et al. 2004;Wilson et al. 2013;Popa et al. 2014;Luo et al. 2016;Vincenot et al. 2017;Cho et al. 2018;Li 2020). However, the species recognition of this genus in China is still in its infancy. ...
Laccaria fagacicola and L. pallidorosea, two new species collected from subtropical broad-leaved forests, were described in this study with both molecular and morphological evidence. Laccaria fagacicola is characterized by its brownish orange basidioma and globose to subglobose, echinulate basidiospores with spines 1–2 μm long and 1–1.5 μm wide at base. Laccaria pallidorosea has a small and pinkish basidioma, and globose to subglobose, echinate basidiospores with spines 1.5–2 μm long and 2 μm wide at base. Phylogenetic analyses based on internal transcribed spacer (ITS) region indicated that L. fagacicola is sister to L. rubroalba and L. aurantia, while L. pallidorosea is related to L. versiforma and L. salmonicolor. These two new species are described, illustrated, and compared with closely related and morphologically similar species.
... Moreover, the species of Asian, Australian, European, and North American origin form separate clades. Similar results have been reported for other species groups in the various ectomycorrhizal genera of Russulaceae, which are often endemic to specific geographical regions or a single continent (Cho et al. 2018;De Crop et al. 2018;Wang et al. 2018;Lee et al. 2019;Looney et al 2020). Their host plants may have acted as bridges for species dispersal and diversification (Looney et al. 2018), and geographic distance and climate disjunctions may have caused species divergence Lee et al. 2019). ...
Russula subsection Amoeninae is morphologically defined by a dry velvety pileus surface, a complete absence of cystidia with heteromorphous contents in all tissues, and spores without amyloid suprahilar spot. Thirty-four species within subsection Amoeninae have been published worldwide. Although most Russula species in South Korea have been assigned European or North American names, recent molecular studies have shown that Russula species from different continents are not conspecific. Therefore, the present study aims to: 1) define which species of Russula subsection Amoeninae occur on each continent using molecular phylogenetic analyses; 2) revise the taxonomy of Korean Amoeninae. The phylogenetic analyses using the internal transcribed spacer (ITS) and multilocus sequences showed that subsection Amoeninae is monophyletic within subgenus Heterophyllidiae section Heterophyllae. A total of 21 Russula A peer-reviewed open-access journal Launched to accelerate biodiversity research Komsit Wisitrassameewong et al. / MycoKeys 75: 1-29 (2020) 2 subsection Amoeninae species were confirmed from Asia, Australia, Europe, North America, and Central America, and species from different continents formed separate clades. Three species were recognized from South Korea and were clearly separated from the European and North American species. These species are R. bella, also reported from Japan, a new species described herein, Russula orientipurpurea, and a new species undescribed due to insufficient material.
