Figure 3 - uploaded by Shah Hussain
Content may be subject to copyright.
Basidiomata of species of Leucoagaricus. A, B. Leucoagaricus badius (holotype). C. Leucoagaricus pakistaniensis (holotype). D. Leucoagaricus lahorensiformis (LAH SHL2). E. Leucoagaricus umbonatus (holotype). F. Leucoagaricus sultanii (holotype).
Source publication
The large genus Leucoagaricus (Basidiomycota) is poorly studied in Pakistan, where the northern parts of the country are considered hotspots for biodiversity. Based on morphological and molecular data, five new species are described: Leucoagaricus badius, L. lahorensiformis, L. pakistaniensis, L. sultanii and L. umbonatus. Descriptions and illustra...
Context in source publication
Context 1
... TEF1-α data set consisted of 36 sequences and 557 sites; after exclusion of ambiguously aligned sites, it consisted of 365 distinctly aligned positions and pro- duced a ML tree with optimized log-likelihood value of −5695.633034 ( SUPPLEMENTARY FIG. 3). The new species were recovered with excellent bootstrap support of ...
Similar publications
Samples of species close to Tremella fibulifera from China and Brazil are studied, and T. fibulifera is confirmed as a species complex including nine species. Five known species ( T. cheejenii , T. fibulifera s.s., T. “ neofibulifera ”, T. lloydiae-candidae and T. olens ) and four new species ( T. australe , T. guangxiensis , T. latispora and T. su...
Fibrodontia is a genus of wood-inhabiting fungi consisting of four species so far, including F. gossypina as generic type. Two new species, Fibrodontia austrosinensis and F. subalba, are described and illustrated from China. Fibrodontia austrosinensis from southwestern China is characterized by a grandinioid to odontioid hymenophore with numerous s...
Taxonomic and phylogenetic studies of Chlorophyllum were carried out on the basis of morphological differences and molecular phylogenetic analyses. Based on the phylogeny inferred from the internal transcribed spacer (ITS), the partial large subunit nuclear ribosomal DNA (nrLSU), the second largest subunit of RNA polymerase II ( rpb2 ) and translat...
Citations
... This genus is typified by Leucoagaricus barssii (Zeller) Vellinga (2000: 431). Several molecular studies of both genera showed that the genus is monophyletic and divided into six sections viz., Annulati, Rubrotincti, Sculpturati, Leucoagaricus, Piloselli and Sphaerocystophori (Singer, 1948, Singer 1986, Vellinga 2004, Vellinga et al. 2011, Liang et al. 2010, Ge et al. 2015, Hussain et al. 2018, Sysouphanthong et al. 2022. ...
... Leucoagaricus is represented by 221 species as per Index Fungorum (9 January 2024) while 288 legitimate names have been found in MycoBank (9 January 2024). They are distributed in Africa (Sysouphanthong et al. 2018), Asia (Kumari & Atri 2013, Yuan et al. 2014, Nabe et al. 2014, Ge et al. 2015, Yu et al. 2016, Yang et al. 2017, Hussain et al. 2018, Verma et al. 2018, Yang et al. 2019, Ashraf et al. 2023, Australia (Aberdeen 1992, Grgurinovic 1997, Europe (Vellinga et al. 2010, Forin et al. 2022, North America (Justo et al. 2015) and South America (Ferreira & Cortez 2012, Heisecke et al. 2022). • Phytotaxa 000 (0) © 2024 Magnolia Press Species in Leucoagaricus are characterized by small to medium, fleshy or thin lepiotaceous basidiomata, ranging in stature from slender to sturdy; a pileus surface that is radially fibrillose, floccose, squamulose to fibrillose-scaly or rarely granulose; entire or very short striate margins; a central, equal to bulbous stipe with a membranous, sometimes moveable annulus; metachromatic, thin-walled, smooth, hyaline basidiospores that generally lack a well-defined germ pore, and a pileipellis that is either a trichoderm or a cutis of repent and radially arranged hyphae lacking sphaerocysts. ...
... From Pakistan, 21 Leucoagaricus species have been reported (Ahmad et al. 1997, Ge et al. 2015, Hussain et al. 2018, Usman & Khalid 2018, Ullah et al. 2020, Asif et al. 2021, Ashraf et al. 2023, Khalil et al. 2023, Khan et al. 2023. The present study focuses on the morphological and molecular characterization of a Leucoagaricus species collected in the Changa Manga Forest, Kasur District Punjab, Pakistan. ...
Leucoagaricus rosuliformis is described as a new species from the Changa Manga Forest, Punjab, Pakistan. This species can be separated from its close relatives, L. lahorensis and L. changamangaensis by its rosette-like central disc and with dense squamules becoming scattered towards margins. Molecular phylogenetic analyses using nuclear ribosomal DNA sequences from the internal transcribed spacer region also supported this taxon as a distinct species.
... Molecular systematics and taxonomic studies so far have revealed 19 species of Leucoagaricus and five species of Leucocoprinus from Pakistan (Ahmad et al. 1997;Ashraf et al. 2023;Asif et al. 2021;Ge et al. 2015;Hussain et al. 2018;Ijaz et al. 2023;Jabeen et al. 2020;Khalil et al. 2023;Khan et al. 2023;Naseer et al. 2023;Qasim et al. 2015;Rehman et al. 2023;Ullah et al. 2019;Usman and Khalid 2018). Here, we describe three new species from southern Punjab, Pakistan, a hitherto unexplored area that supports a large diversity of mushroom species fruiting every year during the monsoon season. ...
... Liu) Asif, Saba & Vellinga reported from China is a close relative of Lc. iqbalii but can be differentiated from the latter due to its bottle-green to dark brown fibrillose squamules, broadly clavate cheilocystidia, ellipsoid basidiospores, and rarely bisporic basidia (Ma et al. 2022 iqbalii. Leucocoprinus gujratensis differs due to its milky white pileus surface with brown furfuraceous squamules, lacrymoid to amygdaliform basidiospores (5.4-8.6 × 3.9-5.5 μm) with abundant clavate to slightly fusiform cheilocystidia, pileipellis with periclinal hyphae to irregularly ascending, and cylindrical and septate hyphae wide as cutis with light brown terminal ends (Rehman et al. 2023), whereas Lc. umbonatus differs from Lc. iqbalii by its applanate to convex pileus, cutis of white fibrils radially arranged, yellowish-pink conspicuous umbo, reflexed margins, stipe almost equal with swollen base, annulus membranous, basidiospores amygdaliform to ovoid, and ventricose cheilocystidia (Hussain et al. 2018). Leucocoprinus acutoumbonatus T.K.A. Kumar & Manim. ...
... Molecular systematics and taxonomic studies so far have revealed 19 species of Leucoagaricus and five species of Leucocoprinus from Pakistan (Ahmad et al. 1997;Ashraf et al. 2023;Asif et al. 2021;Ge et al. 2015;Hussain et al. 2018;Ijaz et al. 2023;Jabeen et al. 2020;Khalil et al. 2023;Khan et al. 2023;Naseer et al. 2023;Qasim et al. 2015;Rehman et al. 2023;Ullah et al. 2019;Usman and Khalid 2018). Here, we describe three new species from southern Punjab, Pakistan, a hitherto unexplored area that supports a large diversity of mushroom species fruiting every year during the monsoon season. ...
... Liu) Asif, Saba & Vellinga reported from China is a close relative of Lc. iqbalii but can be differentiated from the latter due to its bottle-green to dark brown fibrillose squamules, broadly clavate cheilocystidia, ellipsoid basidiospores, and rarely bisporic basidia (Ma et al. 2022 iqbalii. Leucocoprinus gujratensis differs due to its milky white pileus surface with brown furfuraceous squamules, lacrymoid to amygdaliform basidiospores (5.4-8.6 × 3.9-5.5 μm) with abundant clavate to slightly fusiform cheilocystidia, pileipellis with periclinal hyphae to irregularly ascending, and cylindrical and septate hyphae wide as cutis with light brown terminal ends (Rehman et al. 2023), whereas Lc. umbonatus differs from Lc. iqbalii by its applanate to convex pileus, cutis of white fibrils radially arranged, yellowish-pink conspicuous umbo, reflexed margins, stipe almost equal with swollen base, annulus membranous, basidiospores amygdaliform to ovoid, and ventricose cheilocystidia (Hussain et al. 2018). Leucocoprinus acutoumbonatus T.K.A. Kumar & Manim. ...
... In recent years, many papers have been published, describing new species from different fungal groups collected in Pakistan (e.g. Razaq et al. 2012;Nawaz et al. 2013;Thongklang et al. 2014;Qasim et al. 2015aQasim et al. , 2015bSarwar et al. 2015;Hussain et al. 2016Hussain et al. , 2017Hussain et al. , 2018Jabeen et al. 2016;Farooqi et al. 2017;Naseer et al. 2018;Ullah et al. 2018;Saba et al. 2019aSaba et al. , 2019bKiran et al. 2020). Thirty-five species of Inocybe sensu lato have been reported from Pakistan (Ahmad et al. 1997;Ilyas et al. 2013;Saba et al. 2015;Jabeen et al. 2016;Farooqi et al. 2017;Razaq and Shahzad 2017;Naseer et al. 2018;Ullah et al. 2018;Song et al. 2019;this study). ...
Within the family Inocybaceae, many species of Mallocybe have been reported, but there are only a few reports of this genus from Pakistan. In this study, six collections of Mallocybe were studied by morphological and phylogenetic methods. Phylogenetic analyses, based on sequence data from two different loci (ITS and LSU) using Maximum Likelihood and Maximum Parsimony methods, have been performed to infer species relationships within Mallocybe . Results indicated that these six collections encompass two new species of Mallocybe i.e. M. pakistanica and M. pinicola , from Pakistan. Their detailed morphological descriptions and illustrations are also provided. In addition, comparison with morphologically closely-related taxa is also discussed. Previously, only two species of this genus have been recorded from Pakistan and, with this addition, the total number of reported taxa of Mallocybe has been raised to four from Pakistan. A key to the described taxa of Mallocybe from Pakistan is also provided.
... Only 14 species of Leucoagaricus have been reported from Pakistan so far (Ahmad et al. 1997, Ge et al. 2015, Hussain et al. 2018, Usman and Khalid 2018, Ullah et al. 2019, Asif et al. 2021. Based on morphological observations and molecular phylogenetic analyses, this study describes a new species collected from Himalayan moist temperate forests of Swat and Khanspur, KP, Pakistan. ...
A novel mushroom species occurring on humus‐rich soil was collected from Himalayas of Swat and Khanspur, Pakistan. Phylogenetic analyses based on the nrDNA ITS region revealed that it belongs in Leucoagaricus sect. Leucoagaricus . Further morphological analyses confirmed its uniqueness, and here it is described as a new species under the name Leucoagaricus swaticus. The distinguishing characters of this novel taxon are a relatively large pileus with distinct, brownish red umbo, larger, smooth stipe and oblong to amygdaliform basidiospores (6.7–9.0 × 3.0–4.7 µm) with apical papilla. A detailed description of this taxon is provided.
... During last three decades, 12 species of Leucoagaricus have been described from Pakistan based on molecularphylogenetic basis (Ge et al. 2015;Qasim et al. 2015;Hussain et al. 2018;Usman & Khalid 2018;Ullah et al. 2019;Jabeen et al. 2020Rehman et al. 2023), while two species were reported based on morphology solely (Ahmad et al. 1997). More new species can still be discovered, by exploring mycoflora in understudied areas. ...
... La. lahorensis, described from Pakistan differs from La. thallensis due to its smooth stipe surface, absence of volva, thin-walled basidiospores, sub-cylindrical cheilocystidia, and light brownish pileal hyphae The characters of La. lahorensiformis, a species also known from Pakistan, which differentiate it from our taxon include a smooth stipe surface without fibrils, absence of volva, fusiform basidiospores, cylindrical cheilocystidia with crystals at apex, and light orange pileal hyphae (Hussain et al. 2018). ...
A new agaric species, Leucoagaricus thallensis, a member of La. sect. Leucoagaricus is described and illustrated from Paki-stan. This species is mainly recognized by the combination of the orange-red fibrillose pileus with a white background, the fibrils on the stipe, and the volva. Data on macromorphology, micromorphology, DNA sequences (nrITS and nrLSU), habitat and comparison with closely related taxa are provided for each. Morphological and molecular phylogenetic differences separate our species from all other known species of Leucoagaricus proving its novelty. This study is the first contemporary taxonomic work on Leucoagaricus species from Noorpur Thal, district Khushab.
... Taxonomic studies on Leucoagaricus throughout the whole of Pakistan are, in fact, scant. Only 12 species of Leucoagaricus have been reported from Pakistan so far (Ahmad et al. 1997;Ge et al. 2015;Qasim et al. 2015;Hussain et al. 2018;Usman and Khalid 2018;Ullah et al. 2019;Asif et al. 2021). All in all, further research on the diversity of this genus in the whole region is required. ...
The genus of basidiomycetous fungi, Leucoagaricus, occurs worldwide, from subtropical to boreal latitudes. Several collections of Leucoagaricus were made during mycological field trips conducted in different forests of Margalla, Pakistan. An integrative framework combining morphological and phylogenetic data was employed for their study. As a result, the two species La. margallensis and La. glareicolor are here described as new to science. Detailed macro-and micro-morphological descriptions, and a molecular phy-logenetic reconstruction based on nrITS and LSU sequence data are provided and used to discriminate the new species from morphologically and phylogenetically close taxa. Whereas, our phylogenetic tree inference gave unequivocal support for the inclusion of these two species within the section Leucoagaricus.
... ex Singer (1948: 2) is represented by more than 135 species of lepiotaceous and saprotrophic fungi distributed all over the world (Kirk et al. 2008;Asif et al. 2021;Ma et al. 2022;Manawasinghe et al. 2022). Most of the species of Leucoagaricus have been described from temperate regions in North America and Europe, but a few species have been reported from the tropics and sub-tropical regions including Pakistan (Liang et al. 2010;Hussain et al. 2018;Usman & Khalid 2018;Latha et al. 2020). Vellinga recovered a monophyletic group (Leucoagaricus/ Leucocoprinus clade) in which the species of Leucoagaricus and Leucocoprinus were phylogenetically intermixed, but considered polyphyletic on the bases of macro-morphological and phylogenetic studies (Johnson 1999;Vellinga 2004b;Vellinga et al. 2011).The genus is characterized by fragile to stout fleshy squamulose pileus, white to creamy stipe with annulus, pulverulent and slightly non-striate pileus margin, free lamellae, white to cream or pink spore print, dextrinoid spores with cheilocystidia, pleurocystidia, and clamp connections absent in all tissues (Singer 1986;Vellinga 2001). ...
... This research work is the continuation of exploring genus Leucoagaricus in Pakistan (Ge et al. 2015Hussain et al. 2018;Usman & Khalid 2018;Ullah et al. 2019;Asif et al. 2021). During last two decades, 13 species of Leucoagaricus have been reported from Pakistan so far (Ahmad et al. 1997, Hussain et al. 2018Usman & Khalid 2018;Ullah et al. 2019;Jabeen et al. 2020;Asif et al. 2021). ...
... This research work is the continuation of exploring genus Leucoagaricus in Pakistan (Ge et al. 2015Hussain et al. 2018;Usman & Khalid 2018;Ullah et al. 2019;Asif et al. 2021). During last two decades, 13 species of Leucoagaricus have been reported from Pakistan so far (Ahmad et al. 1997, Hussain et al. 2018Usman & Khalid 2018;Ullah et al. 2019;Jabeen et al. 2020;Asif et al. 2021). Basidiomata of current taxon were collected frequently from scrubland of Achh village, Gujrat district during monsoon of 2021-22. ...
A novel mushroom, occurring on sandy soil was collected from scrubland of Gujrat district, Pakistan. Phylogenetic analyses of the nrDNA ITS region revealed that it clusters within Leucoagaricus section Leucoagaricus. Further morpho-anatomical analyses confirmed its uniqueness, and it is here described as Leucoagaricus gujratensis sp. nov. The distinguishing characters of this novel taxon are fragile basidiomata with brown to light brown squamules in the center on milky white pileus surface; creamy stipe with white central annulus; lacrymoid to amygdaliform basidiospores (5.4-8.6 × 3.9-5.5 μm) and clavate to slightly fusiform and fabiform cheilocystidia. The new mushroom resembles La. candidus in appearance but the morpho-anatomical and molecular characters prove its novelty. The number of Leucoagaricus species in Pakistan has reached up to 14.
... ex Singer (1948: 2) is represented by more than 135 species of lepiotaceous and saprotrophic fungi distributed all over the world (Kirk et al. 2008;Asif et al. 2021;Ma et al. 2022;Manawasinghe et al. 2022). Most of the species of Leucoagaricus have been described from temperate regions in North America and Europe, but a few species have been reported from the tropics and sub-tropical regions including Pakistan (Liang et al. 2010;Hussain et al. 2018;Usman & Khalid 2018;Latha et al. 2020). Vellinga recovered a monophyletic group (Leucoagaricus/ Leucocoprinus clade) in which the species of Leucoagaricus and Leucocoprinus were phylogenetically intermixed, but considered polyphyletic on the bases of macro-morphological and phylogenetic studies (Johnson 1999;Vellinga 2004b;Vellinga et al. 2011).The genus is characterized by fragile to stout fleshy squamulose pileus, white to creamy stipe with annulus, pulverulent and slightly non-striate pileus margin, free lamellae, white to cream or pink spore print, dextrinoid spores with cheilocystidia, pleurocystidia, and clamp connections absent in all tissues (Singer 1986;Vellinga 2001). ...
... This research work is the continuation of exploring genus Leucoagaricus in Pakistan (Ge et al. 2015Hussain et al. 2018;Usman & Khalid 2018;Ullah et al. 2019;Asif et al. 2021). During last two decades, 13 species of Leucoagaricus have been reported from Pakistan so far (Ahmad et al. 1997, Hussain et al. 2018Usman & Khalid 2018;Ullah et al. 2019;Jabeen et al. 2020;Asif et al. 2021). ...
... This research work is the continuation of exploring genus Leucoagaricus in Pakistan (Ge et al. 2015Hussain et al. 2018;Usman & Khalid 2018;Ullah et al. 2019;Asif et al. 2021). During last two decades, 13 species of Leucoagaricus have been reported from Pakistan so far (Ahmad et al. 1997, Hussain et al. 2018Usman & Khalid 2018;Ullah et al. 2019;Jabeen et al. 2020;Asif et al. 2021). Basidiomata of current taxon were collected frequently from scrubland of Achh village, Gujrat district during monsoon of 2021-22. ...
A novel mushroom, occurring on sandy soil was collected from scrubland of Gujrat district, Pakistan. Phylogenetic analyses of the nrDNA ITS region revealed that it clusters within Leucoagaricus section Leucoagaricus. Further morpho-anatomical analyses confirmed its uniqueness, and it is here described as Leucoagaricus gujratensis sp. nov. The distinguishing characters of this novel taxon are fragile basidiomata with brown to light brown squamules in the center on milky white pileus surface; creamy stipe with white central annulus; lacrymoid to amygdaliform basidiospores (5.4-8.6 × 3.9-5.5 μm) and clavate to slightly fusiform and fabiform cheilocystidia. The new mushroom resembles La. candidus in appearance but the morpho-anatomical and molecular characters prove its novelty. The number of Leucoagaricus species in Pakistan has reached up to 14.
... Species of Leucoagaricus are terrestrial and saprotrophic, and species are distributed in both tropical and temperate regions (Kirk et al. 2008). Several new species have been described from Asia, such as India (Kumar & Manimohan 2009), China (Liang et al. 2010, Ge et al. 2015, Yang & Ge 2017, Yuan et al. 2014, Yu et al. 2016, Pakistan (Hussain et al. 2018, Qasim et al. 2015, and Laos (Sysouphanthong et al 2018). In this paper, we introduce two new Leucoagaricus species from Laos and Thailand (Fig. 53). ...
... Leucoagaricus crystallifer has basidiomata cream to pale ochre and cheilocystidia cylindrical to fusiform . Leucoagaricus sultanii has yellowish-pink to dark yellowish-pink pileus and relatively wide basidia 21-27 × 7-10 μm (Hussain et al. 2018). Leucoagaricus croceus is also similar to L. croceus in their red basidiomata. ...
... Leucoagaricus croceus is also similar to L. croceus in their red basidiomata. However, L. badius has a clavate to fusiform cheilocysitidia, and has narrower basidia (6-8 μm; Hussain et al. 2018). Based on both morphological and molecular phylogeny we introduce Leucoagaricus croceus as a novel species. ...
This is the seventh in a series of Mycosphere notes in which we provide notes on the collection of fungi isolated from various hosts. In this set of notes, we introduce Pseudophialocephala as a new genus, nine new species, 27 new host or country records and five new combinations. The new species are Ceratosphaeria yunnanensis, Cytospora salicis-albae, Gymnopus bunerensis, Kalmusia cordylines, Leucoagaricus croceus, Leucoagaricus laosensis, Neopyrenochaeta shaoguanica, Pseudophialocephala cuneata and Robillarda aquatica. Pseudophialocephala humicola, Pseudophialocephala aquatica, Pseudophialocephala salinicola, Pseudophialocephala terricola and Pseudophialocephala xalapensis are introduced as new combinations. We provide new molecular data for 43 species belonging to two phyla, three classes and 15 orders. Updated phylogenetic trees for 22 families (Agaricaceae, Botryosphaeriaceae, Chaetosphaeriaceae, Coniochaetaceae, Didymosphaeriaceae, Gloeophyllaceae, Glomerellaceae, Hysteriaceae, Lophiostomataceae, Magnaporthaceae, Neopyrenochaetaceae, Omphalotacea, Phaeosphaeriaceae, Phyllostictaceae, Pleosporaceae, Saccotheciaceae, Savoryellaceae, Sporocadaceae, Stachybotryaceae, Torulaceae, Valsaceae, Physalacriaceae) and 32 genera (Alfaria, Aureobasidium, Ceratosphaeria, Collybiopsis, Colletotrichum, Comoclathris, Coniochaeta, Cytospora, Dothiorella, Gymnopus, Gymnopus, Heliocybe, Hysterium, Hysterobrevium, Kalmusia, Leptospora, Letendraea, Leucoagaricus, Mucidula, Neoleptosporella, Neopyrenochaeta, Paraleptospora, Phyllosticta, Pseudophialocephala, Rhytidhysteron, Robillarda, Savoryella, Sporocadus, Thozetella, Torula and Vaginatispora) are given.
