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Basidiomata of Amanita castanea. a young and mature basidiomata. b–d basidiospores in 5% KOH. f–h basidiospores in congo red. i–k basidiospores in Melzer's reagent. l–o basidia and subhymenium at different stages of development. (a–o: BZ201405, holotype) (scale bar: a = 2 cm, b–k = 6 μm, l–o = 10 μm).
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Mushrooms belonging to the genus Amanita were collected during a fungal biodiversity study in northern Thailand in 2012–2014. Morphological characteristics and molecular phylogenetic analyses were used to identify the mushrooms to species. Amanita castanea is described as new to science and compared with phenetically and phylogenetically similar sp...
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Citations
... Up to now, six taxa of section Phalloideae have been reported from Thailand, namely Amanita ballerina Raspé, Thongbai [25,26,29]. One more taxon is recognized in our phylogenetic analysis, and here it is described as a new species based on morphological evidence as well. ...
... Sacc., A. sculpta Corner and Bas, and A. sinocitrina Zhu L. Yang, Zuo H. Chen and Z.G. Zhang [25,26,29]. In this study, A. cacaina L.P. Tang Habitat: Solitary to scattered on soil in tropical deciduous or coniferous forests dominated by Dipterocarpus, Shorea, and Pinus species, respectively. ...
The Amanita subgenus Amanitina contains six sections, and the species diversity of this subgenus has still not been explored in Thailand. Twenty samples collected in 2019 and 2020, which had the morphological characteristics of the Amanita subgen. Amanitina, were observed in this study. Both the microscopical characteristics and multi-gene phylogenetic analyses of the ITS, nrLSU, RPB2, TEF1-α, and TUB gene regions revealed that the 20 samples represented nine species and dispersed into four sections. Remarkably, three taxa were different from any other currently known species. Here, we describe them as new to science, namely A. albifragilis, A. claristriata, and A. fulvisquamea. Moreover, we also recognized six interesting taxa, including four records that were new to Thailand, viz. A. cacaina, A. citrinoannulata, A. griseofarinosa, and A. neoovoidea, as well as two previously recorded species, A. caojizong and A. oberwinkleriana. Moreover, we provide the first RPB2 and TEF1-α gene sequences for A. cacaina. Detailed descriptions, illustrations as line drawings, and comparisons with related taxa are provided.
... The topology of the phylogenetic trees from the ML and BI analyses were similar. A phylogenetic tree obtained from the ML analysis is represented in Figure 2. Our phylogenetic tree was constructed with the aim of having similar outcomes to previous phylogenetic studies [4,[52][53][54]. The phylogenetic tree consisted of 62 specimens of Amanita sect. ...
... phylogenetic tree was constructed with the aim of having similar outcomes to previous phylogenetic studies [4,[52][53][54]. The phylogenetic tree consisted of 62 specimens of Amanita sect. ...
... Distribution: known from China [4], Japan [61,62], and Thailand [52], this study. Remarks: Morphologically, A. rubroflava is easily confused with A. rubromarginata. ...
Edible wild mushrooms are extremely popular among consumers and are highly valued for their potential economic benefits in northern Thailand. In this present study, a total of 19 specimens of edible Amanita were collected during investigations of wild edible mushrooms in northern Thailand during the period from 2019 to 2022. Their morphological characteristics and the phylogenetic analyses of the internal transcribed spacer (ITS) and partial large subunit (nrLSU) of ribosomal RNA, RNA polymerase II second-largest subunit (rpb2) and partial translation elongation factor 1-alpha (tef-1) indicated that the collected specimens belonged to A. hemibapha, A. pseudoprinceps, A. rubromarginata, A. subhemibapha, and Amanita section Caesareae. This is the first report of A. pseudoprinceps and A. subhemibapha from Thailand. Full descriptions, illustrations and a phylogenetic placement of all specimens collected in this study are provided. Subsequently, the nutritional composition and total phenolic content, as well as the antioxidant and α-glucosidase inhibitory activities, of each species were investigated. The results indicate that the protein contents in both A. pseudoprinceps and A. subhemibapha were significantly higher than in A. hemibapha and A. rubromarginata. The highest total phenolic content was found in the extract of A. pseudoprinceps. In terms of antioxidant properties, the extract of A. pseudoprinceps also exhibited significantly high antioxidant activity by 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) assays. However, the extract of A. rubromarginata had the lowest total phenolic content and level of antioxidant activity. Additionally, α-glucosidase inhibitory activity varied for different Amanita species and the highest level of α-glucosidase inhibitory activity was found in the extract of A. pseudoprinceps. This study provides valuable information on the nutrient content, phenolic content and the antioxidant and α-glucosidase inhibitory potential of edible Amanita species found in northern Thailand.
... Genus Amanita merupakan kelompok jamur Agaricales yang populer baik sebagai bahan pangan maupun jamur beracun di seluruh dunia. Sebagian besar jamur ini diketahui merupakan kelompok mikobion pembentuk ektomikoriza dengan berbagai tumbuhan kehutanan seperti Dipterocarpaceae dan lain-lain (Zhang et al., 2015;Thongbai et al., 2016). Amanita merupakan jamur pembentuk ektomikoriza yang memiliki spesies paling banyak di dunia. ...
... Genus Amanita memiliki persebaran yang luas mulai dari wilayah tropis, subtropis, hingga negara empat musim (Zhang et al., 2015;Tang et al., 2017;Assyov et al., 2021). Hutan-hutan di negara Asia seperti Jepang, China, India, Thailand, dan Malaysia merupakan salah satu tempat yang memiliki keragaman spesies Amanita yang tinggi (Rokuya et al., 2011;Deng et al., 2014;Thongbai et al., 2016;Tang et al., 2017). Sementara itu, di Indonesia, informasi komprehensif mengenai Amanita hanya pernah dilaporkan oleh Boedijn (1951) yang melaporkan sebanyak 11 spesies Amanita dari Pulau Sumatra dan Jawa. ...
... Hal ini dapat terwujud dengan adanya kolaborasi yang baik antara berbagai instansi terkait. Kolaborasi riset tersebut dapat meningkatkan peluang ditemukannya catatan baru atau spesies baru Amanita asal Indonesia, seperti halnya di beberapa negara di Asia (Kim et al., 2013;Thongbai et al., 2016;Tang et al., 2017;Saba et al., 2019). ...
Ectomycorrhizal fungi play an important role in a forest ecosystem. The genus of Amanita is one of the common ectomycorrhizal fungi in the forest. The information on the diversity of Amanita in Indonesia is not properly organized. This is due to most of the prior publications were not equipped with basidiomata descriptions. The current research aimed to inventory the diversity of Amanita and provide a description of the basidiomata from the Haurbentes Research Forest, West Java. Macrofungi exploration was carried out by the opportunistic sampling method and identification of fungi based on morphological characters. A total of seven species of Amanita were successfully described and identified in this study : Amanita sp.1, Amanita sp.2 sect. Vaginatae, Amanita sp.3 sect. Vaginatae, Amanita cf. alboflavescens, Amanita cf. virginea, Amanita cf. fulva and Amanita cf. sychnopyramis.This report is the first information of Amanita in research site and adds to the record of mushroom diversity in Indonesia.
... The elongate to ventricose basal bulb is characteristic for both non-lethal subclades. However, the main morphological characteristic that differentiates the species of subclade II from those of subclade III is their appendiculate pileal margins (Yang et al. 2001;Thongbai et al. 2016;Cui et al. 2018). All those morphological characteristics of the non-lethal species do not fit the circumscription of A. sect. ...
The members of Amanita sect. Phalloideae (Fr.) Quél. are responsible for many fatalities worldwide. However, some species in
this section have previously been reported as non-lethal and lacking deadly toxins. Sequences of five genes (ITS, nrLSU, RPB2,
TEF1-α, TUB2) of species belonging to the section from tropical Africa, America, Asia, Australia, and Europe were included in
this study to elucidate the phylogenetic relationships among the species. The results indicated that the lethal species are in one
clade (subclade I) while the non-lethal species are divided into two clades (subclades II and III) within the section. Moreover, two
non-lethal species from tropical Africa, namely A. ballerinoides and A. bulbulosa are newly described based on both morphology
and molecular approaches. Phylogenetically, they cluster in the same subclade III with other known non-lethal amanitas,
including A. ballerina, A. chuformis, A. franzii, A. levistriata, and A. pseudogemmata. Neither amatoxins nor phallotoxins were
detected in A. ballerinoides and A. bulbulosa by LC-HRMS, which agrees with their placement in the non-lethal subclade III
within A. sect. Phalloideae. Finally, a key to the West African species of Amanita sect. Phalloideae is provided.
... Amanita Pers. is a widespread basidiomycetous genus comprising more than 600 species all over the world [1][2][3][4][5]. According to recent studies [3][4][5], this genus was proposed to be divided into three subgenera and eleven sections [subgenus Amanita Pers., containing: section Amanita Pers., section Amarrendiae (Bougher & T. Lebel) Zhu L. Yang [4,[6][7][8][9][10][11][12]. ...
... mira, A. obsita, A. siamensis, and A. subglobosa, have only morphologic data [6], which need to be supplemented with more molecular data to confirm their taxonomic status. Our study, along with other previous studies, allowed to discover the high diversity of Amanita species in Thailand [6][7][8][9][10][11][12], indicating that more taxa remain to be discovered and documented. ...
Based on a survey of macro-fungi in northern and northeastern Thailand, nine samples collected in 2020 are identified as Amanita and introduced here as two new species, Amanita kalasinensis and A. ravicrocina. Typical macro- and microscopical characteristics indicate that both of these two species belong to Amanita section Amanita, but differ from other currently known species. Amanita kalasinensis is characterized by having a greyish yellow pileus covering with a conical to granuliform, yellowish white volval remnant; the presence of clamps; and a broadly ellipsoid to ellipsoid basidiospore. Amanita ravicrocina is characterized by having a brown to greyish orange pileus covering with a patchy, white volval remnant; a collar-like volval remnant on the stipe; and a subglobose to broadly ellipsoid basidiospore. Multi-gene phylogenetic analysis of partial nuclear rDNA internal transcribed spacer region (ITS), partial nuclear rDNA large subunit region (nrLSU), RNA polymerase II second largest subunit (RPB2), partial translation elongation factor 1-alpha (TEF1-α), and beta-tubulin gene (TUB) also revealed that positions of A. kalasinensis and A. ravicrocina are well-supported within A. section Amanita, but form distinct lineages and do not show any close relationship with any species. The detailed morphological features, line-drawing illustration, and comparison with morphological similar taxa are provided.
... To date, 51 taxa of Amanita, including twelve species from section Amanita, namely, A. aff. mira Corner & Bas, A. (Sanmee et al. 2008;Li et al. 2016;Thongbai et al. 2016Thongbai et al. , 2017Thongbai et al. , 2018Phookamsak et al. 2019). Among these species, Amanita gleocystidiosa and A. pyriformis were treated as synonyms of A. sychnopyramis f. subannulata Hongo and A. orientigemmata Zhu L. Yang & Yoshim. ...
Amanita submelleialba sp. nov. in section Amanita, is described from northern Thailand based on both multi-gene phylogenetic analysis and morphological evidences. It is characterized by having small to medium-sized basidiomata; a yellow to yellowish pale pileus covering pyramidal to subconical, white to yellow white volval remnants; globose stipe base covered conical, white to yellow white volval remnants; fugacious subapical annulus; and absent clamps. Multi-gene phylogenetic analyses based on partial nuclear rDNA internal transcribed spacer region (ITS), partial nuclear rDNA larger subunit region (nrLSU), RNA polymerase II second largest subunit (RPB2), partial translation elongation factor 1-alpha (TEF1-α) and beta-tubulin gene (TUB) indicated that A. submelleialba clustered together with A. elata and A. mira, but represented as a distinct lineage from other extant species in section Amanita. The detailed morphological characteristics, line-drawing illustration and comparisons with morphologically similar taxa are provided.
... Amanita Pers. is one of the most speciose and widespread agaric genera in tropical ectotrophic forests (Verbeken and Buyck 2002;Brearley 2012;Corrales et al. 2018). However, only around 60 of the globally estimated ~1000 Amanita species are currently known from the African tropics (Tulloss 2005; Thongbai et al. 2016). From 1923 to 1956, Mme. ...
Four epitypes and three new species of Amanita (Amanitaceae, Agaricales, Agaricomycetes, Basidiomycota) are described from Guineo-Congolian rainforests of Cameroon. Amanita echinulata, A. fulvopulverulenta, A. robusta, and A. bingensis are epitypified based on collections that are the first since the species were described nearly a century ago. Morphological features of the epitypes are described and enumerated. Amanita minima, Amanita luteolamellata, and A. goossensfontanae are described as new and added to the known macromycota of tropical Africa. Habit, habitat, and known distribution are provided for each species. Sequence data for the internal transcribed spacer (ITS) locus are provided for types and other collections of all taxa, and a molecular phylogenetic analysis across the genus Amanita corroborates morphology-based infrageneric placement for each.
... The recently described A. castanea Thongbai, Tulloss, Raspé & K.D. Hyde in Thongbai et al. (2016: 215) from Thailand, is easily recognized by its chestnut-coloured pileus, small to medium-sized basidiomata (pileus 50-75 mm wide), a subglobose to hemispheric bulb with pale green tinge on top of the volval limb and greenish grey or greenish yellow skirt-like partial veil that is darker greyish green at the thicker free edge, and smaller basidiospores, (7.2-) 7.3-9.4 (-9.7) × (6.3-) 6.5-8.7 (-9.4) μm (Thongbai et al. 2016). ...
... et al. 2012) and compared to those available in GenBank database (https://www.ncbi.nlm.nih.gov/genbank/) by using the BLASTN algorithm(Altschul et al. 1990). Our datasets consist of sequences of Amanita available in GenBank and UNITE (https://unite.ut.ee/) selected based on BLASTN search and previous molecular studies dealing with Amanita(Thongbai et al. 2016, Jabeen et al. 2019, Mighell et al. 2019. Two phylogenetic analyses were performed: the first phylogenetic analysis, based on nrITS sequences, includes sequences of Amanita sect. ...
... The recently described A. castanea Thongbai, Tulloss, Raspé & K.D. Hyde in Thongbai et al. (2016: 215) from Thailand, is easily recognized by its chestnut-coloured pileus, small to medium-sized basidiomata (pileus 50-75 mm wide), a subglobose to hemispheric bulb with pale green tinge on top of the volval limb and greenish grey or greenish yellow skirt-like partial veil that is darker greyish green at the thicker free edge, and smaller basidiospores, (7.2-) 7.3-9.4 (-9.7) × (6.3-) 6.5-8.7 (-9.4) μm (Thongbai et al. 2016). ...
... et al. 2012) and compared to those available in GenBank database (https://www.ncbi.nlm.nih.gov/genbank/) by using the BLASTN algorithm(Altschul et al. 1990). Our datasets consist of sequences of Amanita available in GenBank and UNITE (https://unite.ut.ee/) selected based on BLASTN search and previous molecular studies dealing with Amanita(Thongbai et al. 2016, Jabeen et al. 2019, Mighell et al. 2019. Two phylogenetic analyses were performed: the first phylogenetic analysis, based on nrITS sequences, includes sequences of Amanita sect. ...
Multigene molecular analysis (nrITS, nrLSU, tef1, rpb2) of Amanita species of section Validae including Italian and French specimens of A. citrina var. intermedia indicates that the taxon is not conspecific with A. mappa (= A. citrina) and deserves to be upgraded to the rank of autonomous species. An in-depth description of the taxon, a comparison with allied species, and a key to the Amanita species of section Validae series Mappae in Europe, are provided
... (Amanitaceae, Agaricales, Basidiomycota) is a large genus comprising about 1000 described species that are important economically and ecologically (Bas 2000, Tulloss 2005, Yang 2005, Kirk & al. 2008, Li & al. 2015. Amanita contains both edible and poisonous species, and some species form ectomycorrhizal associations with vascular plants, which play a key role in maintaining healthy ecosystems (Zhang & al. 2004(Zhang & al. , 2010Thongbai & al. 2016;. Poisonous Amanita species are classified mostly in A. sect. ...
Amanita subjunquillea and its ectomycorrhizal association are reported for the first time from moist temperate Himalayan forests of Pakistan. The sample was studied based on morphological characters and nucleotide sequence analyses of the ITS region generated from basidiomata and ectomycorrhizal roots of Quercus floribunda. Our collection differs from the type in its dark orange pileus disc and pale yellow margins. Remaining morphological and molecular data are consistent with previously reported specimens. This represents the first report of A. subjunquillea from Pakistan.
