Basic microscopic and macroscopic features of Serratia marcescens strain Tom1. (A) Microscopic (phase contrast) appearance of typical mid-exponential cells, (B) Typical red coloration of overnight colonies on plate count agar, (C) Phenotypic demonstration of an inducible AmpC-β-lactamase using the MAST 69C kit.

Contexts in source publication

Context 1

... Gram-and oxidase-negative strain Tom1 formed motile rods (~2 × 0.9 µm), was β-galactosidase-and catalase-positive, hydrolyzed gelatin, and formed typical red colonies evidencing the production of prodigiosin (Fig. 1A and B). The assembled genome yielded 22 contiguous sequences (> 300 bp), was 4950426 bp long, and had a G+C content of 59.83%. The corresponding N 50 , N 75 , L 50 , and L 75 values were 1349891 bp, 327775 bp, 2, and 5, and the longest contig measured 1399301 bp. The genome was 99.32% complete based on universal singlecopy ortholog analysis, ...

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... product absorbing at 486 nm, confirming β-lactamase activity. Furthermore, the presence of an inducible AmpC-β-lactamase was demonstrated as the strain showed resistance against cefpodoxime in the presence of an AmpC inducer and a combination of an AmpC inducer and an ESBL inhibitor but not in the presence of an AmpC inhibitor (Mast D69C, Fig. 1C). In addition, the negative result obtained using the Mast D63C extended-spectrum-β-lactamase detection kit for AmpC-producing Enterobacterales matched the absence of known ESBL genes on the draft genome of S. marcescens strain Tom1. Thus, the phenotypic resistance profile of S. marcescens Tom1 confirmed its genome-based prediction as ...

Context 3

... ability of strain Tom1 to synthesize the pigment prodigiosin, yielding the typical red colonies (Fig. 1B), matched the presence of the prodigiosin biosynthesis gene cluster detected on the genome. The red pigment prodigiosin originating from Serratia species was reported to inhibit the growth of various Gram-negative and Gram-positive bacteria [10] and, additionally, to exhibit immunosuppressive properties [3,10]. Fig. 2. Midpoint-rooted ...

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... Gram-and oxidase-negative strain Tom1 formed motile rods (~2 × 0.9 µm), was β-galactosidase-and catalase-positive, hydrolyzed gelatin, and formed typical red colonies evidencing the production of prodigiosin (Fig. 1A and B). The assembled genome yielded 22 contiguous sequences (> 300 bp), was 4950426 bp long, and had a G+C content of 59.83%. The corresponding N 50 , N 75 , L 50 , and L 75 values were 1349891 bp, 327775 bp, 2, and 5, and the longest contig measured 1399301 bp. The genome was 99.32% complete based on universal singlecopy ortholog analysis, ...

Context 5

... product absorbing at 486 nm, confirming β-lactamase activity. Furthermore, the presence of an inducible AmpC-β-lactamase was demonstrated as the strain showed resistance against cefpodoxime in the presence of an AmpC inducer and a combination of an AmpC inducer and an ESBL inhibitor but not in the presence of an AmpC inhibitor (Mast D69C, Fig. 1C). In addition, the negative result obtained using the Mast D63C extended-spectrum-β-lactamase detection kit for AmpC-producing Enterobacterales matched the absence of known ESBL genes on the draft genome of S. marcescens strain Tom1. Thus, the phenotypic resistance profile of S. marcescens Tom1 confirmed its genome-based prediction as ...

Context 6

... ability of strain Tom1 to synthesize the pigment prodigiosin, yielding the typical red colonies (Fig. 1B), matched the presence of the prodigiosin biosynthesis gene cluster detected on the genome. The red pigment prodigiosin originating from Serratia species was reported to inhibit the growth of various Gram-negative and Gram-positive bacteria [10] and, additionally, to exhibit immunosuppressive properties [3,10]. Fig. 2. Midpoint-rooted ...