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The antibacterial effects of Neem leaf, the growth performance and sensory qualities of African catfish fed with Neem leaf inclusion were investigated in this study. Ninety (90) post-juvenile Clarias gariepinus (42.40± 2.50 g) were used. Experimental groups in triplicate were exposed bi-weekly to 3.5% and 7% LC50 of Neem leaves aqueous extract infu...
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Context 1
... spp. and Bacillus subtilis were not isolated in the gills (Table 4) but were present in the skin (Table 5). Escherichia coli, Bacillus megaterus and Proteus spp. in the gills were resistant to the Neem leaf extract at both concentrations (Table 4), while Aeromonas hydrophila was only susceptible at the 7% (T3) concentration in the gill. ...Context 2
... Bacillus subtilis were not isolated in the gills (Table 4) but were present in the skin (Table 5). Escherichia coli, Bacillus megaterus and Proteus spp. in the gills were resistant to the Neem leaf extract at both concentrations (Table 4), while Aeromonas hydrophila was only susceptible at the 7% (T3) concentration in the gill. In the skin sample, E. coli and Pseudomonas fluorescens were resistant to Neem leaf extract at both concentrations, Micrococci spp. ...Context 3
... spp. and Bacillus subtilis were not isolated in the gills (Table 4) but were present in the skin (Table 5). Escherichia coli, Bacillus megaterus and Proteus spp. in the gills were resistant to the Neem leaf extract at both concentrations (Table 4), while Aeromonas hydrophila was only susceptible at the 7% (T3) concentration in the gill. ...Context 4
... Bacillus subtilis were not isolated in the gills (Table 4) but were present in the skin (Table 5). Escherichia coli, Bacillus megaterus and Proteus spp. in the gills were resistant to the Neem leaf extract at both concentrations (Table 4), while Aeromonas hydrophila was only susceptible at the 7% (T3) concentration in the gill. In the skin sample, E. coli and Pseudomonas fluorescens were resistant to Neem leaf extract at both concentrations, Micrococci spp. ...Similar publications
The aim of this study is to observe and assess the performance of Psychrotrophic Gram-negative bacteria, namely Pseudomonas fluorescens, which pose a significant spoilage problem in food, under different temperature, water activity (aw) and pH conditions. Noting that at aw 0.6 and 0.75 irrespective of temperature or pH and at temperatures 60 0 and...
Plant root-associated microbes promote plant growth and elicit induced systemic resistance (ISR) to foliar pathogens. In an attempt to find novel growth-promoting and ISR-inducing strains, we previously identified strains of root-associated Pseudomonas spp. that promote plant growth but unexpectedly elicited induced systemic susceptibility (ISS) ra...
A. johnsonii and P. fluorescens are the well-known specific spoilage organisms in aquatic products and the study of the interactions between A. johnsonii and P. fluorescens are limited. This study aims to evaluate the growth kinetics, spoilage potential and interactions of A. johnsonii and P. fluorescens isolated from spoiled bigeye tuna (Thunnus o...
The bio-fertilizer potential of Trichoderma viride and Pseudomonas fluorescens on growth and yield performance of open pollinated maize variety Rampur Composite was studied at the research farm of Agriculture and Forestry University (AFU), Rampur, Chitwan, Nepal during the winter season of 2018/19. The experiment was laid out in randomized complete...
Citations
... With this production rise, aquaculture services are progressively depending on the input of formulated feeds and disease-resistant drugs for increased production. 2 Fish is a principal supply for humans, especially as source of animal protein, digestible minerals, commercial (profit-making) or subsistence (survival) farming. 3,4 Fin (Tilapia, Catfishes) and Shell (Oysters, Clams, Mussels) fishes serve as effective genetic replica for the evaluation of effluence in aquatic ecosystems 5 and can play major roles in assessing imminent vulnerability allied with contagion in aquatic ecosystem since they are directly exposed to chemicals (trace or heavy) resulting from agricultural production via surface overspill or tactfully, through the food chain of the ecosystem. ...
The indiscriminate use of plant extracts as antimicrobial agent on culturable fish (catfishes and
the Cichlids) in confinement is alarming, especially the use of neem leave extracts. Neem
(Azadirachta indica) is a medicinal plant containing active biological and chemical properties.
The effects of aqueous Azadirachta indica (neem)extract on the cytology and genome
composition of hundred post juvenile Oreochromisniloticuswith mean length and weight of
937±0.67cm and 899±1.84 g respectively was investigated in a tank culture system to
determine the median lethal concentration (LC50) at 96 h of exposure. Five graded
concentrations of 12, 14, 16, 18 and 20 L of the aqueous extract and control were diluted in
15 L of water in triplicate. The control group and group exposed to 1.2 L concentration of neem
extract showed normal behaviour while group exposed to 1.6 and 1.8 L concentrations showed
abnormal behavioural changes such as rolling movement, gulpingof air, weakness, swimming
on the back and eventually death was observed. There was significant difference (p<0.05) in the
toxicity effects of neem extract at 14thand 28thday of exposure. Cytotoxic effect was evident by
cellular apoptosis which increases with increase concentration of the extract while genotoxic
effect was manifested by formation of nuclear aberrations within the erythrocytes. The results
obtained in this study revealed that neem extract has clastogenic and apoptotic effects on the
erythrocytes of Oreochromis niloticus.
With the increasing occurrence of bacterial resistance against available antibiotics, it has now become necessary to investigate newer sources for antimicrobials. Endophytic fungi have the aptitude to co-exist with their host plants without affecting any harm and are useful to both the plant and the fungi. The current study aimed to evaluate the antimicrobial activity of the bioactive compounds of some endophytic fungi isolated from five medicinal plants against P. aeruginosa. A total of 30 distinct colonies of the endophytic fungi were isolated from five selected medicinal plants. Most of
the isolates were obtained from H. sabdariffa L., O. europaea L., M. piperita L., M. oleifera Lam. and A. indica A. Juss. Most of the fungal isolates belonged to Alternaria sp., Cladosporium sp., Ulocladium sp., Chaetomium sp., A. niger, E. nidulans and Fusarium sp. A total of 34 intra- and extra-cellular metabolites from 17 endophytic fungal isolates were evaluated for their antibacterial activities against P. aeruginosa, some endophytic fungal metabolites showed antibacterial activities as A. niger extract isolated from neem which exhibited the most potent antibacterial activity16.5± 0.71 mm against P. aeruginosa. In addition, Cladosporium, sp. isolated from neem, Chaetomium sp. extracts and Alternaria sp. (isolated from mint), showed antibacterial activity with the zone of inhibition, 13± 1.41, 12.5± 0.71and 12.5± 0 mm, respectively. In contrast, none of the Ulocladium sp., Alternaria sp. extracts (isolated from olive), Cladosporium sp., and E. nidulans extracts (isolated from mint) showed any antibacterial activities against P. aeruginosa
