Figure 1 - uploaded by Uma Gaur
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BBR attenuated the decrease in cell viability induced by H 2 O 2 in PC12 cells. (A) The structure of BBR. (B) Cells were incubated with H 2 O 2 (25-400 μM) or 0.1% DMSO (vehicle control) for 24 h, then the cell viability was determined by the MTT assay. (C) Cells were treated with BBR (0.3-10 μM) or 0.1% DMSO (vehicle control) for 24 h and cell viability was measured using the MTT assay. (D) Cells were pre-treated with BBR at indicated concentrations, then incubated with or without 200 μM H 2 O 2 for further 24 h. Data represented means ± SD, * P<0.05 vs. control group; # P<0.05 vs. the H 2 O 2-treated group.
Source publication
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Oxidative stress is a much-recognized phenomenon linked with the progression of Neurodegenerative Diseases (NDs) due to imbalances in redox homeostasis. Increasing evidence indicates that excessive Reactive Oxygen Species (ROS), impairing the physiological functions of neurons via inducing cell apoptosis, is the main cause of NDs. The drug candidat...
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... calculate the cytotoxicity of H 2 O 2 , PC12 cells were incubated with different concentrations of H 2 O 2 for 24 h and the cell viability was measured by MTT assay. As shown in Figure 1, H 2 O 2 treatment for 24 h significantly decreased the cell viability of PC12 cells in a concentration-dependent manner. H 2 O 2 at 200 μM caused about 20% loss in cell viability, thus this concentration of H 2 O 2 was used in the coming experiments to produce the cell insult and to measure neuroprotection. ...
Citations
... Several in vitro and in vivo studies have been showing the therapeutic efficacy of berberine against Parkinson's disease, likely being the natural alkaloid with the largest potential for the prevention or management of neurodegenerative conditions [111][112][113][114][115][116][117][118]. Berberine has demonstrate exceptional antioxidant properties as free radical scavenging, Fe 2+ chelating activity, inhibition of ROS and NOS production, and regulation of endogenous antioxidants [21]. ...
Complex diseases such as neurodegenerative disorders and cancer constitute a growing public health problem due to the rising incidence and lack in effective therapies. Since pharmacotherapy based on a single target has been insufficient for drug development in complex diseases, the emerging multi-target approach is a promising strategy for the search of new drug candidates. Plant-derived isoquinoline alkaloids comprise a vast source of multimodal agents with unique structural diversity, and variated range of pharmacological activities. This review offers an exhaustive compilation of the pharmacological relevance and multi-target potential of natural isoquinolines, emphasizing their features and promising activity in complex diseases such as Alzheimer, Parkinson, and Cancer. Selected examples were discussed in depth to illustrate the most relevant structural motifs and their possible relationship with the multimodal activity offering a comprehensive baseline in the search and optimization of isoquinoline scaffolds with polypharmacological potential for complex diseases.
