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Automontage images of Glyptapanteles creatonoti (Viereck). Female in dorsal view (A), mesosoma with metasoma in part (B) metasoma along with mesosoma in part (C), wings (D).
Source publication
Glyptapanteles Ashmead (Hymenoptera: Braconidae: Microgastrinae) is a cosmopolitan group of hyperdiverse parasitic wasps. The genus remains taxonomically challenging in India due to its highly speciose nature, morphological similarity amongst species and negligible host records. The Indian fauna is one of the most diverse and also the least studied...
Citations
... The eggs laid by the adult moths were used for maintaining a test insect to carry out the laboratory experiments. Further, the identity of the insect was confirmed through amplification of cytochrome oxidase I gene (COI) marker (Gupta et al. 2016). The nucleotide sequence was submitted to NCBI GenBank data base, and accession number was obtained (accession number MK041922). ...
Background
Maize fall armyworm, Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae), is a highly migratory polyphagous insect pest that has posed itself as a very threatened insect invaded India since May 2018 and devastated the maize crop. This insect pest caused 62.5% damage on maize plants in Hassan district of Karnataka state, India. Surveys were undertaken in different parts of the country to assess the natural occurrences of all three categories of entomopathogens (bacteria, fungi, and viruses) associated with fall armyworm. Maximum occurrence of infection of entomopathogens (15.13%) was recorded from Chikkaballapura, Karnataka, followed by 12.23% from Hassan, Karnataka.
Results
Totally 13 isolates of S. frugiperda nucleopolyhedrovirus (SpfrNPV) were collected from 13 locations of the country. Electron microscopy studies clearly showed the tetrahedral shaped occlusion bodies of SpfrNPV with the size of 1.48–1.68 µm. The identity of highly virulent SpfrNPV NBAIR1 (Chikkaballapura isolate) was confirmed using conserved polyhedrin gene-specific primers and NCBI GenBank accession number was obtained (MT422725). Bioassay studies revealed that the SpfrNPV NBAIR1 Chikkaballapura isolate was highly virulent with the highest larval mortality (95.50%) and the lowest LC 50 value of 2.11 × 10 ³ OBs/ml. SpfrNPV is exclusively host specific and did not infect any other insect species other than S. frugiperda tested in this present study.
Conclusions
Among 13 isolates of SpfrNPV, SpfrNPV NBAIR1 Chikkaballapura isolate was highly virulent with respect to the larval mortality. Hence SpfrNPV NBAIR1 Chikkaballapura isolate is having a great potential to play in the management of maize fall armyworm S. frugiperda .
... divergent from the closet BIN in the database (BOLD:AAH1268, Glyptapanteles creatonoti (Viereck, 1912)). We have examined the images of G. creatonoti in Gupta et al. (2016) and are confident that G. kurandaensis sp. nov. is a different species, most easily separated by the following morphological characteristics: G. kurandaensis sp. ...
... nov. has T1 completely black, whilst G. creatonoti has the anterior area of T1 pale ("stamineous" in original description, appearing pale orange in images of Gupta et al. (2016)). ...
Despite several decades of active research, there are still substantial gaps in the knowledge of parasitoid wasps in Australia, with many families and genera yet to be revised using modern approaches and only a fraction of the estimated fauna currently described. The genus Glyptapanteles Ashmead, 1904 is a member of the subfamily Microgastrinae (Hymenoptera: Braconidae) and all species in the subfamily are lepidopteran parasitoids. The genus previously contained only three species known from Australia: G. deliasa Austin & Dangerfield, 1992, G. drioplanetus Fagan-Jeffries & Austin, 2021 and G. mnesampela Austin, 2000. To undertake a revision of this morphologically-conserved group in Australia, we used a combination of molecular (cytochrome oxidase subunit one (COI) and wingless genes) and minimal morphological data to delimit and describe an additional 31 species: G. austini Fagan-Jeffries & Bird sp. nov. and the following 30 species all authored by Fagan-Jeffries, Bird & Austin: G. albigena sp. nov., G. andamookaensis sp. nov., G. arcanus sp. nov., G. aspersus sp. nov., G. austrinus sp. nov., G. baylessi sp. nov., G. bradfordae sp. nov., G. cooperi sp. nov., G. doreyi sp. nov., G. dowtoni sp. nov., G. eburneus sp. nov., G. erucadesolator sp. nov., G. ferrugineus sp. nov., G. foraminous sp. nov., G. goodwinnoakes sp. nov., G. guzikae sp. nov., G. harveyi sp. nov., G. kingae sp. nov., G. kittelae sp. nov., G. kurandaensis sp. nov., G. lambkinae sp. nov., G. lessardi sp. nov., G. mouldsi sp. nov., G. niveus sp. nov., G. rixi sp. nov., G. rodriguezae sp. nov., G. ruhri sp. nov., G. sanniopolus sp. nov., G. vergrandiacus sp. nov. and G. wrightae sp. nov. We provide a key to species groups and to the species able to be identified on morphological characters alone. Additionally, we provide a brief discussion of the difficulties in describing small, morphologically conserved wasps and the challenges associated with revising the taxonomy of hyperdiverse taxa in the context of the planned mission of Taxonomy Australia to accelerate the documentation of Australia’s biodiversity.
... Varatharajan et al. (1998) also reported that G. obliquae and Carcelia sp, were found to parasitize S. obliqua to the extent of 6% and 4%, respectively. G. obliquae is a promising gregarious endoparasitoid, specific to S. obliqua (Gupta et al. 2016). The parasitoid usually prefers to lay the eggs in the early instars of its host and kill them before they complete the larval stage (Brown et al. 1993;Soller and Lanzrein 1996;Harvey et al. 2000). ...
The Bihar hairy caterpillar, Spilarctia obliqua, is a dreaded and sporadic pest on several cash crops in the Oriental region. The polyphagous feeding habit enables it to survive on a variety of crops. The group of hairy caterpillars of this study was found on young plants of the sweet basil. Young larvae feed gregariously on the under surface of the leaves later it feed solitary in nature. Damage symptoms, as skeletonization of the upper epidermis of the leaves, were observed. The identity of the pest species confirmed through morphological and molecular methods (No. MK491177). The phylogenetic analysis indicates the distribution of uniform populations of S. obliqua infesting soybean and sweet basil. The total life cycle was longest during winter (male, 68.67: female, 73.27 d) compared to Kharif season (male, 49.37; female, 53.74 d). The damage incidence ranged between 13.33–60% and 10–63.33% in 2018 and 2019, respectively. The natural enemy populations Glyptapanteles obliquae (11%) and Carcelia sp (17%) were recorded. This is the first report of S. obliqua on sweet basil, Ocimum basilicum, in India.
... Homolobinae are solitary koinobiont parasitoids and parasite larvae of Lepidoptera especially Geometridae and Noctuidae that feed exposed at night [5]. Distribution in the world: Diverse Microgastrinae genus with species distributed worldwide [42]. ...
... Biology: The genus includes solitary or gregarious koinobiont endoparasitoid wasps of macro lepidoptera families [42]. Distribution in the world: The species of the genus Opius are distributed worldwide [14]. ...
A faunistic inventory of Hymenoptera Braco-nidae from two olive orchards in Tiaret province (Algeria) is contributed. These insects were collected by Barber pots and yellow plates methods from December 2018 to November 2019. A total of 13 species of Braconidae from 9 genera and 7 sub-families are listed. Nine taxa are new record to the Algeria entomofauna: Aspilota sp, Aphidius ab-sinthii, Aphidius setiger, Bracon intercessor, Bracon variator, Chelonus planiventris, Homolobus pul-chricornis, Glyptapanteles sp. and Opius sp. This inventory provides a preliminary database on Braconid diversity in a semi-arid habitat.
... The eggs laid by the moths were used for maintaining a culture of tested insect to carry out the laboratory experiments. Further, the identity of the pest was confirmed through amplification of cytochrome oxidase I gene (COI) marker (Gupta et al. 2016). The nucleotide sequence was submitted to NCBI GenBank data base (accession number MK531549). ...
Jute hairy caterpillar, Spilosoma (=Spilarctia) obliqua (Walker) (Arctiidae: Lepidoptera), is an irregular and
polyphagous insect pest that occurs all over India. A severe natural viral epizootic was observed in the field
population of S. obliqua in jute. The virus was characterized as Spilosoma obliqua nucleopolyhedrovirus (SpobNPV
NBAIR1) based on morphological, biological, and molecular characterization. Under scanning and transmission
electron microscopy (SEM and TEM), the occlusion bodies (OBs) of SpobNPV NBAIR1 appeared as both tetrahedral
and triangular with the size ranged from 1.04-1.72 μm. The results on the bioassay studies revealed that the lowest
LC50 value of 2.93 OBs/mm2 by leaf disc bioassay against the second instar S. obliqua showed its high virulence.
The identity of SpobNPV was further confirmed by the amplification of polyhedrin gene (NCBI GenBank accession
no. MK288145). Eleven species of lepidopteran insects, viz., Spodoptera litura, S. exigua, S. frugiperda, Helicoverpa
armigera, Plutella xylostella, Amsacta albistriga, Maruca vitrata, Trichoplusia ni, Pieris brassicae, Agrotis ipsilon, and
Bombyx mori, were not susceptible to the infection with SpobNPV NBAIR1. Field experiments on jute crop revealed
68.92, 78.59, and 93.16% reduction in larval population of S. obliqua at 3, 4, and 7 days post spray of SpobNPV
NBAIR1 (1.5 × 1012 OBs/ha at 2 ml/l), respectively. The present study is a renewed effort in the management of S.
obliqua by the new strain of SpobNPV NBAIR1.
... Glyptapanteles is a cosmopolitan group of hyper-diverse parasitoid species, which occur in all faunal regions (Whitfield et al. 2018) worldwide including Australia (Austin and Dangerfield 1992), Ecuador (Whitfield et al. 2002), China (Chen and Song 2004;Zeng et al. 2007), Greece (Papp 2007), Croatia, Bosnia and Macedonia (Papp 2009), India (Sathe and Dawale 1999; Gupta and Pereira 2012; Gupta and Fernández-Triana and Ward 2014; Gupta et al. 2011Gupta et al. , 2016, and remains taxonomically challenging worldwide due to its highly specious nature, morphological similarity amongst species and negligible host records. Recent specimens from Neotropical regions indicates this genus has the most diverse species within Microgastrinae containing Glyptapanteles, Apanteles Förster, and Diolcogaster Ashmead (Whitfield et al. 2018). ...
... Until 2018, more than 122 species were described worldwide (Yu et al. 2016, Gupta et al. 2016, then 136 new species of Glyptapanteles from Costa Rica and Ecuador were described (Arias-Penna et al. 2019). Unfortunately, hundreds of species of Glyptapanteles remain undescribed. ...
The south-east coastal area of Fujian, China, belongs to the Oriental Realm, and is characterized by a high insect species richness. In this work, a new species of Hymenopteran parasitoid, Glyptapanteles gigas Liang & Song, sp. nov. found in Jinjiang within hosts of caterpillars Macrobrochis gigas (Lepidoptera: Arctiidae), is described and illustrated, with differences from similar species. Additionally, we presumed that both parasitoid and host species play very important role in the coevolution and tritrophic interaction between plants, phytophagous insects, and their parasitoids, because these insects probably broke the sporangia and made contributions to their colonization, or some spores were spread for long distances by adult moths after their emergence, or some parasitoids were attracted by the eggs and larvae of these caterpillars, which was also thought to be helpful to spread of spores.
... India-present in mainland as well as in Andaman & Nicobar Islands (Gupta & Fernández-Triana, 2014). Gupta et al., (2016) have emphasized on the use of integrated approach (combining morphology, host data, and COI gene) as a dependable method of species delimitation for the identification of microgastrine wasps (Braconidae) as they are super diverse and highly speciose. Hence molecular characterization was performed for C. ruficrus. ...
Field surveys conducted during 2018-2019 in the maize fields infested with Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae) in Karnataka, Tamil Nadu, Rajasthan and Meghalaya revealed that Cotesia ruficrus (Haliday) (Hymenoptera: Braconidae) was the common gregarious larval parasitoid in the maize fields parasitizing S. frugiperda. This is the first report of C. ruficrus parasitizing S. frugiperda in India, earlier reports being from Trinidad and Tobago. The present study (using integrated approach) provides morphological and molecular identification details along with host data, cocoon characters and geographical distribution of C. ruficrus.
... Small broods with two or three individuals are known in a few cases, e.g., C. astrarches (Marshall, 1889) with 3-6 indiviuals in the host Aricia Reichenbach, 1817 (Lepidoptera Linnaeus, 1758: Lycaenidae Leach, 1815) (Shaw 2012;Quicke 2015). Gupta et al. (2016a) used the gregarious species of the genus Glyptapanteles Ashmead, 1904 to generate accurate boundaries between species/species-groups using an integrated approach with three different sets of data (morphology, host records and mitochondrial cytochrome c oxidase subunit I (COI) nucleotide sequences). Gupta & Fernández-Triana (2014) and Fernández-Triana et al. (2014) recorded many gregarious and solitary hosts associated with Cotesia from the Oriental and Neotropical regions, respecively. ...
The present study is based on the genus Cotesia Cameron,1891 collected from Khuzestan Province in the Southwestern part of Iran during 2016-2017. Nine species (+200 specimens) of the genus Cotesia were collected and identified. We recognised three new species, which we describe and illustrate here: Cotesia elongata Zargar & Gupta sp. nov., C. khuzestanesis Zargar & Gupta sp. nov. and C. zagrosensis Zargar & Gupta sp. nov. Two species are recorded for the first time from Iran: Cotesia cynthiae (Nixon, 1974) and C. glabrata (Telenga, 1955). A faunistic list with species distribution in Iran, a modified key to include the new species and brief diagnoses for the new records from Iran are provided.
... India-present in mainland as well as in Andaman & Nicobar Islands (Gupta & Fernández-Triana, 2014). Gupta et al., (2016) have emphasized on the use of integrated approach (combining morphology, host data, and COI gene) as a dependable method of species delimitation for the identification of microgastrine wasps (Braconidae) as they are super diverse and highly speciose. Hence molecular characterization was performed for C. ruficrus. ...
... As the majority of the BOLD sequences for these two genera were based on mitochondrial Cytochrome Oxidase I (COI) gene, the same was chosen for the present study. Recently KEY WORDS: Acanthodelta janata, barcode, COI, Microplitis maculipennis, Snellenius (Article chronicle: Received: 07-08-2018;Revised: 19-11-2018;Accepted: 01-12-2018 molecular based identification of insects using mitochondrial Cytochrome Oxidase I gene (COI) is gaining importance due to shortfalls in morphology-based identification (Erlandson et al., 2017;Venkatesan et al., 2016;Gupta et al., 2016). ...
... In our previous molecular-based studies, it was confirmed that the DNA barcoding of trichogrammatids (Hymenoptera: Trichogrammatidae) by using the mitochondrial cytochrome oxidase-I marker sequences was a practical approach for shaping molecular diversity (Venketesan et al., 2016). In addition to that, the phylogenic analysis was performed to identify and classify various species-groups of the genus Glyptapanteles Ashmead, 1904 (Insecta: Hymenoptera: Braconidae: Microgastrinae) (Gupta et al., 2016). ...
Microplitis maculipennis Szépligeti is an important parasitoid of castor semilooper Acanthodelta janata (L.) (Lepidoptera),
a major pest of castor (Ricinus communis L.). Microplitis Förster shares remarkable morphological resemblance with moderately diverse genus Snellenius Westwood. In this study, molecular characterization of M. maculipennis was done using Cytochrome Oxidase I (COI) to confirm its generic placement in the respective genus. The Bayesian Inference (BI) and Maximum Likelihood (ML) phylogenetic analysis performed with a total of 354 published BOLD database sequences (after pre-processing of a total of 2257 COI sequences) of Microplitis and Snellenius species, representing 129 named species and 226 species determined only to genus raises doubts on the retention of both these genera separately. Our studies reveal that COI gene could not discriminate Microplitis and Snellenius species clearly.
