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![Fig. 3 BV-biofilm [31, 38]. A bacterial biofilm, an adhering microbial...](publication/332234722/figure/fig2/AS:744505716256768@1554515403033/BV-biofilm-31-38-A-bacterial-biofilm-an-adhering-microbial-community-enveloped-by_Q320.jpg)
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Introduction
Bacterial vaginosis (BV) is the most common vaginal disorder in reproductive-age women. The condition is characterised by the replacement of a healthy, lactobacilli-dominated vaginal microbiota by anaerobic and facultative anaerobic bacteria. BV increases the risk of acquisition of STIs and is associated with pregnancy complications. A...
Contexts in source publication
Context 1
... first described A. vaginae isolated from the vagina of a healthy women in Sweden. These are Gram-positive, elliptical or rod-shaped cocci, nonmotile and non-spore-forming organisms, and occur alone, in pairs, in clumps or in short chains (Fig. 1). They produce major amounts of lactic acid next to acetic and formic acids and are strictly ...
Context 2
... first described A. vaginae isolated from the vagina of a healthy women in Sweden. These are Gram-positive, elliptical or rod-shaped cocci, nonmotile and non-spore-forming organisms, and occur alone, in pairs, in clumps or in short chains (Fig. 1). They produce major amounts of lactic acid next to acetic and formic acids and are strictly ...
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Oral consumption of probiotics is practical and can be an effective solution to preserve vaginal eubiosis. Here, we studied the ability of orally administered Lactobacillus paracasei LPC-S01 (DSM26760) to affect the composition of the vaginal microbiota and colonize the vaginal mucosa in nondiseased adult women. Forty volunteers took oral probiotic...
Bacterial vaginosis (BV) is associated with a highly structured polymicrobial biofilm on the vaginal epithelium where Gardnerella species presumably play a pivotal role. Gardnerella vaginalis, Atopobium vaginae, and Prevotella bivia are vaginal pathogens detected during the early stages of incident BV. Herein, we aimed to analyze the impact of A. v...
Background: Recent studies on the urine microbiome have highlighted the importance of the gut-vagina-bladder axis in recurrent cystitis (RC). In particular, the role of Gardnerella as a covert pathogen that activates E. coli in animal experiments has been reported. Herein, we conducted a human bladder microbiome study to investigate the effect of G...
Koch's postulates dictate the use of experimental models to illustrate features of human disease and provide evidence for a singular organism as the cause. The underlying cause(s) of bacterial vaginosis (BV) has been debated in the literature for over half a century. In 1955, it was first reported that a bacterium now known as Gardnerella vaginalis...
Bacterial vaginosis (BV) has been reported in one-third of women worldwide at different life stages, due to the complex balance in the ecology of the vaginal microbiota. It is a common cause of abnormal vaginal discharge and is associated with other health issues. Since the first description of anaerobic microbes associated with BV like Gardnerella...
Citations
... Fannyhessea vaginae (formerly Atopobium vaginae) is an anaerobic Gram-positive coccus most frequently isolated from the female genital tract in association with bacterial vaginosis [1,2]. In recent years, there have been several reports of invasive disease with Fannyhessea vaginae occurring in females, generally related to gynaecological conditions or surgery [3]. ...
... Fannyhessea vaginae is a Gram-positive, obligate anaerobic bacteria of the family Atopobiaceae within the order Coriobacteriales, and appears as cocci or coccobacilli in pairs or short chains [1,4,5]. It is fastidious, non-motile, catalase-negative [1] and non-spore forming [2,4]. After originally being described as Atopobium vaginae in 1999, phylogenetic analysis prompted reclassification of the organism to the new genus Fannyhessea in 2018 of which F. vaginae is the type organism [1,6]. ...
... After originally being described as Atopobium vaginae in 1999, phylogenetic analysis prompted reclassification of the organism to the new genus Fannyhessea in 2018 of which F. vaginae is the type organism [1,6]. It is predominantly found in the female genital tract, constituting normal flora in up to 25 % of women [2,7], however it is more commonly found in women with bacterial vaginosis (BV) often in combination with overgrowth of other BV-associated organisms, particularly Gardnerella vaginalis, with which it can form synergistic biofilms [2]. Fannyhessea vaginae has also been recognised as a urinary tract colonising organism [7][8][9]. ...
Introduction. Fannyhessea vaginae (formerly Atopobium vaginae ) is an anaerobic organism commonly associated with female genital flora, with rare cases of invasive disease reported in females.
Case report. We discuss the case of an 81-year-old male who presented with an acute history of back pain and signs of urinary tract infection in the context of intermittent self-urinary catheterisation. Multiple blood cultures grew Fannyhessea vaginae with a later finding of lumbar vertebral osteomyelitis as the cause of back pain. Treatment was commenced with ampicillin, later switched to ceftriaxone, with improvement of acute signs of infection.
Conclusion. Gram-positive anaerobic organisms including Fannyhessea vaginae are possibly under-recognised causes of urinary tract particularly in older males. These bacteria may prove challenging to grow in standard protocols for urine culture; anaerobic or extended incubation could be considered particularly in complicated cases of urinary tract infection without an identifiable pathogen.
... Atopobium spp. belongs to the Coriobacterium family and is an anaerobic microorganism that can produce large amounts of lactic acid [104]. Its pathogenic mechanism may be similar to Lactobacillus spp. ...
... [93][94][95][96][97][98][99][100][101][102][103] Atopobium spp. [104] Clostridium spp. [105] Escherichia coli [106][107][108][109] Campylobacter spp. ...
Purpose
Stomach and esophageal cancers are among the highest mortality from cancers worldwide. Microbiota has an interplaying role within the human gastrointestinal (GI) tract. Dysbiosis occurs when a disruption of the balance between the microbiota and the host happens. With this narrative review, we discuss the main alterations in the microbiome of gastroesophageal cancer, revealing its potential role in the pathogenesis, early detection, and treatment.
Results
Helicobacter pylori plays a major role the development of a cascade of preneoplastic conditions ranging from atrophic gastritis to metaplasia and dysplasia, ultimately culminating in gastric cancer, while other pathogenic agents are Fusobacterium nucleatum, Bacteroides fragilis, Escherichia coli, and Lactobacillus. Campylobacter species (spp.)’s role in the progression of esophageal adenocarcinoma may parallel that of Helicobacter pylori in the context of gastric cancer, with other esophageal carcinogenic agents being Escherichia coli, Bacteroides fragilis, and Fusobacterium nucleatum. Moreover, gut microbiome could significantly alter the outcomes of chemotherapy and immunotherapy. The gut microbiome can be modulated through interventions such as antibiotics, probiotics, or prebiotics intake. Fecal microbiota transplantation has emerged as a therapeutic strategy as well.
Conclusions
Nowadays, it is widely accepted that changes in the normal gut microbiome causing dysbiosis and immune dysregulation play a role gastroesophageal cancer. Different interventions, including probiotics and prebiotics intake are being developed to improve therapeutic outcomes and mitigate toxicities associated with anticancer treatment. Further studies are required in order to introduce the microbiome among the available tools of precision medicine in the field of anticancer treatment.
... Atopobium türleri; oral kavite mikrobiyotasında bulunan ve daha önce laktobasilluslar içerisinde sınıflandırılan gram pozitif anaerop basillerdir. Son yıllardaki çalışmalarda en sık bakteriyel vajinozis (BV) etkeni olarak kabul edilmektedir.Özellikle laktobasillusların hidrojen peroksit üretimi, vajinal pH ve flora üzerinde etkileri sonucu olarak Atopobium kolonizasyonu ve buna bağlı bakteriyel vajinozis geliştiğini bildiren çalışmalar mevcuttur (22) . ...
... F. vaginae is a Gram-positive, elliptical-or rod-shaped coccobacillus that can appear as single elements, pairs, clumps, or short chains and is a part of the human vaginal microbiome. Many studies have emphasized that F. vaginae plays an important role in the pathophysiology of vaginal diseases (Mendling et al., 2019). F. vaginae is able to incorporate into Gardnerella vaginalis biofilms, a crucial marker of bacterial vaginosis (Castro et al., 2021). ...
... It has also been determined that high vaginal loads of F. vaginae in conjunction with Gardnerella spp. is linked to late miscarriage and preterm birth (Bretelle et al., 2015). Although F. vaginae can be detected in the normal vaginal microbiome (8%-25%), it is more prevalent in patients with bacterial vaginosis (50%-96%) (Mendling et al., 2019). However, F. vaginae is an uncommon cause of invasive infections. ...
... However, anaerobic bacteria continue to be among the most neglected and unrecognized pathogens because their cultivation necessitates substantial microbiological experience, and many hospitals (particularly in developing nations) may lack the equipment necessary to achieve anaerobiosis (Nagy et al., 2018). As an anaerobic, F. vaginae is found in the normal vaginal microbiota but is increasingly linked to bacterial vaginosis (Mendling et al., 2019). Recently, a prospective investigation linked F. vaginae to salpingitis and infertility, indicating this microorganism's potential pathogenicity (Haggerty et al., 2016). ...
Bloodstream infection caused by anaerobic microorganisms continues to be associated with a high mortality risk, necessitating a rapid diagnosis and an appropriate treatment. As an anaerobic gram-positive organism associated with vaginal infections, Fannyhessea vaginae is a rare cause of invasive infections. In this case, a 32-year-old pregnant woman with bacterial vaginosis presented with bacteremia. The microbiological analysis of the blood cultures identified F. vaginae. The patient was treated empirically with 5 days of cefoperazone/sulbactam and recovered well. Here, we provide a review of the literature on F. vaginae infections, and the reported cases demonstrate the need for awareness of the different anaerobic species found in the vaginal tract and adaptation of empirical therapies, especially in pregnant women.
... Consequently, infections with sexually transmitted bacteria and bacterial vaginosis can find an excellent niche for implantation. Gardnerella vaginalis and Atopobium vaginae have been considered markers of abnormal vaginal microbiota [4], as they actively contribute to the generation of an unstable microenvironment [5,6]. Moreover, increased microbial diversity is also associated with viral persistence and progression to cervical cancer in oncogenic human papillomavirus (HPV) infections [7]. ...
... B. conglomeratum was found in 58% of the analysed exudates (58/99). HPV detection showed 30% positive cases (30/99), with 19/99 (19%) presenting oncogenic HPV types (39, 45, 53, 56, 58, 59, 73, 82, 66-39, 51-69) and 11/99 (11%) presenting low-risk HPV (6,42,43,44,54,61,70). For sexually transmitted bacteria, 64/99 (65%) were positive, with U. parvum being the most frequent (49%), followed by U. urealyticum (14%). ...
Brachybacterium conglomeratum, traditionally considered an environmental bacterium, has recently garnered attention for its potential involvement in human health. While prior research hinted at its pathogenic role in humans, our study aims to determine its prevalence and associations in diverse clinical contexts. We examined vaginal swabs from three distinct patient groups: patients with low-grade squamous intraepithelial lesions (LSIL), patients with cervicovaginal infections, and patients with a history of precancerous lesions undergoing follow-up. B. conglomeratum was present in all three patient groups, with the highest prevalence observed in the LSIL group. Statistically significant associations were primarily identified in the LSIL group, where B. conglomeratum was present in 60% of cases. Notably, the LSIL group exhibited coinfections with multiple high-risk oncogenotypes of human papillomavirus (HPV), suggesting potential synergistic effects, and understanding these microbial relationships and their influence on viral persistence, particularly with HPV, holds promise for mitigating HPV-related carcinogenesis. Furthermore, Gardnerella vaginalis and Atopobium vaginae were frequently detected in this group, along with Ureaplasma parvum as the predominant sexually transmitted bacterium. In all cases, B. conglomeratum was found in association with these microorganisms rather than as a sole pathogen. This coexistence underscores the intricate microbial interactions within cervicovaginal infections and precancerous lesions. This study marks the first report of B. conglomeratum prevalence in women with these clinical conditions.
... The ad-ministration of tinidazole should be avoided during pregnancy. Because of their clinically comparable efficacy, other antiseptics such as dequalinium chloride or octenidine may be considered suitable alternatives [71,73]. Povidone-iodine should not be administered during pregnancy. ...
... Die Gabe von Tinidazol während der Schwangerschaft sollte vermieden werden. Aufgrund der klinisch vergleich- bar guten Wirksamkeit können alternativ auch Antiseptika wie Dequaliniumchlorid oder Octenidin als geeignete Alternativen angesehen werden [71,73]. Auf die Gabe von Povidon-Jod sollte während der Schwangerschaft jedoch verzichtet werden. ...
Aim This official guideline was coordinated and published by the DGGG, OEGGG and SGGG with the involvement of additional professional societies. The aim of the guideline is to evaluate the relevant literature and use it to provide a consensus-based overview of the diagnosis and management of bacterial vaginosis.
Methods This S2k-guideline was developed by representative members from different medical professional societies on behalf of the guidelines commission of the above-listed societies using a structured consensus process.
Recommendations This guideline provides recommendations on the diagnosis, management, counselling, prophylaxis, and other aspects related to bacterial vaginosis.
... Some authors supported that G. vaginalis is the most frequent bacteria responsible for BV and a good predictor of infection; however, other microorganisms may be involved 6,14 . Indeed, the combination of G. vaginalis with A. vaginae is highly associated with bio lm formation causing recurrence and therapeutic failures[5][6][7]11,12 . Due to the different etiologies of the infection and the diverse bacteria involved, the algorithm for the diagnosis of BV is still under discussion. ...
Vaginitis is a common clinical disorder for which women seek medical care. A clinical evaluation of the Vaginal Panel Realtime PCR kit (qPCR test) compared with reference methods was performed. A total of 1011 vaginal swab specimens were analyzed. The reference methods were Gram strain-based Nugent score combined with Gardnerella culture for bacterial vaginosis (BV) diagnosis and culture for Candida species and Trichomonas vaginalis detection. Candida species identification was performed using matrix-assisted laser desorption ionization-time of flight mass spectrometry. The agreement between both methods for BV was 93.11% with a Kappa value of 0.82 (95% confidence interval 0.781–0.860). The sensitivity, specificity, positive predictive value and negative predictive value of the qPCR test compared to the reference method for Candida species were 96% (95% confidence interval 92.8%-98.1%), 98.4% (95% confidence interval 97.2%-99.2%), 95.3% (95% confidence interval 92.0%-97.3%) and 98.7% (95% confidence interval 97.6%-99.3%), respectively. The qPCR test yielded a positive result for T. vaginalis in twelve of the specimens however the parasite was not microscopically observed in any cultured specimens. The Vaginal Panel Realtime PCR kit showed an optimal agreement compared to reference methods for vaginitis diagnosis, even improving the detection of T. vaginalis .
... For the pre-vaccination set, forty-nine (49) and forty (40) previously con rmed SARS-CoV-2 positives and negatives, respectively, were randomly selected from samples received prior to the availability of vaccines in March 2021 in Ghana through the UN-partnered COVAX initiative (18, 19). SARS-CoV-2 detection was performed with standard RT-qPCR using the Veri-Q PCR 316 COVID-19 Detection Kit (MiCo Biomed Corporation, South Korea), with Ct values < 40 reported as positive. ...
... Several of these taxa have species that are opportunistic pathogens. A. vaginae, the most popularly studied Atopobium species, has been implicated in increased pH (47) and pathogenesis in bacterial vaginosis (48,49). Although these bacteria were not identi ed to the species level in the current study or in others that have also found associations with SARS-CoV-2 infection, their general lactic acid-producing characteristics and the formation of bio lms (50) support their links with the naso-oropharynx in COVID-19 infections. A. parvulum has been implicated in 'long COVID' (51). ...
Background: Microbiome dysbiosis is associated with various diseases, including COVID-19. The association of the oral microbiome with SARS-CoV-2 infections and disease progression has been documented in European, Asian, and American populations but not in Africa.
Methods: We conducted a study in Ghana to evaluate and compare the naso-oropharyngeal microbiome in SARS-CoV-2-infected and noninfected persons before and after vaccination. 16S rDNA was sequenced and analysed from DNA extracted from the naso-oropharyngeal swabs of consenting participants.
Results: Alpha diversity was high among pre-vaccinated virus-positive individuals (Shannon: p< 0.0001) but reduced among vaccinated persons. Contrary to other reports, differences in viral loads did not significantly affect alpha diversity. Pre-vaccinated SARS-CoV-2-positive and -negative individuals had little yet significant microbial compositional dissimilarity (PERMANOVA: R²=0.14, p= 0.001) but not when some individuals were vaccinated (PERMANOVA: R²=0.013, p= 0.49). Consistent with other studies, Prevotella and Atopobium were abundant in pre-vaccinated virus-positive persons (adjusted p value <0.05). Butyrate-producing microbes, including members of Lachnospiraceae and Fusobacterium sp., were in relatively high abundances in infected individuals. As biomarkers associated with the infection (log10LDA> 4.0), they suggest probable protective pathophysiological processes that would prevent severe disease outcomes in this population. Anaerovoracaceae was increased in infected vaccinated persons, further implicating Firmicutes in protective immunity against COVID-19.
Conclusion: Our results necessitate further studies to confirm the integral role of Firmicutes in immune responses and disease progression. We also recommend expansion of microbiome–disease association studies across Africa to identify possible bacterial-mediated therapeutics for emerging infections.
... A. vaginae, a member of the vaginal microflora, is a strict anaerobic, Gram-positive, catalase-negative, rod-shaped coccus [3], and is usually associated with bacterial vaginosis [4]. It should be noted, however, that there have been reports of A. vaginae being isolated from blood cultures from individuals experiencing transient bacteraemia [5], infective endocarditis [6] and sepsis [7]. ...
Skin flora bacteria, such as Cutibacterium acnes , are the predominant contaminants of blood products used for transfusion. Platelet concentrates (PCs), a therapeutic product used to treat patients with platelet deficiencies, are stored at ambient temperature under agitation, providing ideal conditions for bacterial proliferation. At Canadian Blood Services, PCs are screened for microbial contamination using the automated BACT/ALERT culture system. Positive cultures are processed and contaminating organisms are identified using the VITEK 2 system. Over a period of approximately 2 years, several PC isolates were identified as Atopobium vaginae to a high level of confidence. However, since A. vaginae is associated with bacterial vaginosis and is not a common PC contaminant, a retrospective investigation revealed that in all cases C. acnes was misidentified as A. vaginae . Our investigation demonstrated that the media type used to grow PC bacterial isolates can have a significant impact on the results obtained on the VITEK 2 system. Furthermore, other identification methods such as matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALD-TOF MS) and PCR amplification of the 16S RNA gene were only partially successful in the identification of C. acnes . Therefore, our findings support a multiphasic approach when PC isolates are identified as A. vaginae by the VITEK 2 system for proper identification of C. acnes using macroscopic, microscopic and other biochemical analyses.
... The Propionibacterium acnes (present name Cutibacterium acnes) anaerobic bacteria, believed to play a significant role in the pathogenesis of acne vulgaris and prosthetic joint infections [60,61], were identified only in Warszawa 1 soil and Warszawa 2 flowers, i.e. in places with high population density. In contrast, anaerobic Atopobium vaginae, an important component of the complex abnormal vaginal flora in bacterial vaginosis [62,63], was present in samples from all tested places. In our samples, the most common aerobic bacterial species were Sphingomonas paucimobilis, a rare infectious agent which may cause both nosocomial and community-acquired infections, albeit rarely [64,65], Bacillus cereus, producing the heat-stable toxin cereulide known to cause food-borne illness [66,67], and Alloiococcus otitidis, a common bacterium of the human ear; however, while it has been found in a high percentage of middle ear effusions in children, it is still not clear whether this bacterium really is a pathogen [68]. ...
A range of analytical methods (GC-MS, LC-MS, voltammetry, microbiological and microscopic techniques, PCR) was used to assay a range of potential chemical and biological contaminants in soil and dandelion samples. The results provide the first comprehensive safety analysis of dandelion as a herbal product. Samples were collected from three different sites in Poland where the local population collects dandelion plants for their own consumption: Rudenka (a mountain meadow in the European Ecological Network of Natura 2000 protection area, free of agrotechnical treatments for over 30 years), Warszawa 1 (dense single-family housing with heavy traffic), and Warszawa 2 (recreation area with heavy traffic near a coal-fired heat and power plant). The assays of heavy metals and other chemical pollutants (PAHs, PCBs, dioxins, pesticides, mycotoxins) confirm that all collected soil and dandelion samples were chemically pure; however, 95 species of pathogenic bacteria were detected, including "carnivorous" Vibrio vulnificus, zoonotic Pasteurella pneumotropica, Pasteurella canis, Staphylococcus pseudintermedius, Staphylococcus lentus and Francisella tularensis as well as 14 species of pathogenic fungi and one protozoan parasite (Giardia intestinalis). The discovery of septicemia agents V. vulnificus, Fusobacterium mortiferum and Rahnella aquatilis in the soil surrounding dandelion roots and in the flowers, G. intestinalis in dandelion leaves and roots samples, all collected in Warsaw, is highly disturbing. This finding underlines the need for increased caution when collecting dandelion in densely populated areas with a large population of pets. Thorough washing of the harvested plants is necessary before using them for consumption, especially in the case of making salads from fresh dandelion leaves, which is becoming increasingly popular among people leading healthy and an environmentally friendly lifestyle.
