Figure 2 - uploaded by Asha Janadaree Dissanayake
Content may be subject to copyright.
Aplosporella hesperidica (GZAAS 19-1814). (a-c) Conidiomata on host surface. (d,e) Vertical hand sections of multiloculate conidiomata. (f,g) Developing conidia attached to conidiogenous cells. (h) Hyaline immature conidium. (i-k) Mature brown conidia. (l,m) Five-dayold culture on PDA (OXOID CM0139) from above and below. Scale bars: (d,e) = 100 μm, (f-k) = 10 μm.
Source publication
Botryosphaeriales is an important order of diverse fungal pathogens, saprobes, and endophytes distributed worldwide. Recent studies of Botryosphaeriales in China have discovered a broad range of species, some of which have not been formerly described. In this study, 60 saprobic isolates were obtained from decaying woody hosts in southwestern China....
Contexts in source publication
Context 1
... isolates (20% of total isolates) were treated together in an overview phylogenetic tree and seven of them did not cluster with any known Botryosphaeriales species, thus, three novel species were identified based on the morphological and phylogenetic evidence (Figure 1). In this phylogeny, the isolates obtained in the study were clustered with Aplosporella hesperidica (Figure 2), Barriopsis tectonae (Figure 3), Botryobambusa guizhouensis sp. nov. ...
Similar publications
Although Apiospora Sacc. has previously been considered a sexual morph of Arthrinium species on the basis of phylogenetic, morphological, and ecological diagnoses, a recent study delimited these as different species. Recently, 14 species, including eight new species, of marine Arthrinium have been reported from Korea. Six known species have previou...
Citations
... Given that D. seriata pathogenicity was less severe than that of the other isolated Botryospaheriacae, the results indicated above confirmed our findings. Based on other studies, D. seriata is a main pathogen in grapevines in Australia, South Africa, Chile, and France that manifests vascular signs such dark streaking in the wood and cankers (Amponsah et al. 2011;Bruez et al. 2013 (Martin and Cobos 2007;Ramírez et al. 2018;Dissanayake et al. 2021). In terms of D. neojuniperi, it was previously mentioned as being connected to saprobic woody hosts in China in 2017 (Dissanayake et al. 2021) and with leaf blight symptoms on Juniperus chinensis in Thailand (Trakunyingcharoen et al. 2015). ...
... Based on other studies, D. seriata is a main pathogen in grapevines in Australia, South Africa, Chile, and France that manifests vascular signs such dark streaking in the wood and cankers (Amponsah et al. 2011;Bruez et al. 2013 (Martin and Cobos 2007;Ramírez et al. 2018;Dissanayake et al. 2021). In terms of D. neojuniperi, it was previously mentioned as being connected to saprobic woody hosts in China in 2017 (Dissanayake et al. 2021) and with leaf blight symptoms on Juniperus chinensis in Thailand (Trakunyingcharoen et al. 2015). To our knowledge, this is the first record of D. neojuniperi associated with grapevine trunk diseases in Morocco and worldwide. ...
For decades, the grapevine has been produced as a commercial fruit crop in Morocco. From 2004 to 2022, the area dedicated to cultivating and producing grapes has increased across the country. Despite the implementation of adequate agricultural practices, grapevine trunk diseases (GTDs) lead to significant yield losses and a remarkable reduction in viticultural quality. Many grape growers encounter this issue in their vines each year and seek strategies to cope with it. In the most significant grapegrowing regions globally, including Morocco, grapevine decline is a common and significant disease that is brought on by a multitude of latent fungal plant diseases. Due to the use of poorly informative markers in phylogenetic analyses and the lack of relevant morphological characteristics, the classification of these pathogens has proven to be challenging. Numerous genera and families have not yet been extensively studied, especially in Morocco. To identify the dieback-causing agents, surveys were carried out in 152 Moroccan vineyards in Marrakech-Safi, Casablanca-Settat, RabatSale-Kenitra, and Fez- Meknes regions between 2019-2021. Samples of symptomatic trunks were collected and processed for mycological analysis. Afterward, fungal identification of representative isolates was performed combining morphological characterization and phylogenetic analysis based on the internal transcribed spacer (ITS) region of ribosomal DNA and protein-coding gene (β-TUB). Pathogenicity tests were fulfilled under greenhouse conditions for 15 days. Among the fungi collected in the current study, 54 fungal isolates were obtained belonging to different genera and families namely: Botryosphaeria (11 species), Fusarium (10 species), Pestialiotiopsis (4 species), Clonostachys (3 species), Entoleuca mammata, Phaeoacremonium hungaricum, Trichothecium roseum, Diaporthe ampelina, Epicoccum nigrum, Alternaria alternata, and Rosellinia convexa. Pathogenicity test revealed that Diplodia mutila, Neoscytalidium novaehollandiae, Neopestalotiopsis vitis, and Trichothecium roseum isolates are the most pathogenic. This is the first detailed report of species associated with GTDs in Morocco where new records of species worldwide are highlighted.
... This study is a continuation of studies on terrestrial hyphomycete fungi in Guizhou Province in China (Ariyawansa et al. 2016;Chen et al. 2018Chen et al. , 2020Dissanayake et al. 2021;Du et al. 2022;Wu et al. 2023;Zhang et al. 2023). Two new species of Rhodoveronaea are introduced in this study with full descriptions, illustrations, and phylogenetic results and are compared with similar taxa. ...
During a microfungi investigation carried out in a terrestrial forest in Guizhou Province in China, hyphomycetes on a decaying wood log in a terrestrial habitat were collected. Morphological characteristics and phylogenetic analyses of combined ITS, LSU, SSU, tef1-α, and rpb2 sequence data revealed that the collected hyphomycetes represent two distinct new species in Rhodoveronaea. The two new species introduced in this paper are Rhodoveronaea hyalina and R. lignicola. Full descriptions, illustrations, and a phylogenetic tree to show the placement of the two new species are provided.
... Studies on Botryosphaeriaceae, dealing with the phylogenetic traits and morphology of isolates associated with various hosts, have increased in recent years, enabling the worldwide identification of taxa at the species level [2,5,18,[45][46][47][48][49]. In this study, 16 Botryosphaeriaceae isolates were obtained from several decaying woody hosts (dead arial twigs, branches, stems, bark, and seed pods) in southwestern China and northern Thailand, and they were identified as 11 species based on a polyphasic approach of morphological features and molecular phylogeny. ...
Species in the Botryosphaeriaceae are common plant pathogens, endophytes, and saprobes found on a variety of mainly woody hosts. Botryosphaeriaceae is a high-profile fungal family whose genera have been subjected to continuous revisions in recent years. Surveys conducted during 2019 and 2020 on several decaying woody hosts (from dead arial twigs, branches, stems, bark, and seed pods) in China and Thailand revealed a high diversity of Botryosphaeriaceae fungi. Identification of 16 Botryosphaeriaceae isolates was carried out based on both morphological characteristics and phylo-genetic analyses of combined ITS, LSU, tef1-α, and tub2 sequence data. Four novel species (Dothiorella ovata, Do. rosacearum, Do. septata, and Lasiodiplodia delonicis) and seven previously known species (Botryosphaeria fujianensis, Diplodia mutila, Di. seriata, L. crassispora, L. mahajangana, Macrophomina euphorbiicola and Sphaeropsis eucalypticola) were identified while new hosts and geographical records were reported. This study indicates that the fungal family Botryosphaeriaceae seems to be common and widespread on a broad range of hosts in China and Thailand.
... The geographic distribution and host range of botryosphaeriaceous taxa are diverse. Seven genera in Botryosphaeriaceae: Botryosphaeria, Diplodia, Dothiorella, Lasiodiplodia, Neodeightonia, Neofusicoccum and Phaeobotryon are common and frequently reported from various geographical regions (Batista et al. 2021), while Botryobambusa, Oblongocollomyces, Sakireeta and Sardiniella appear to be limited to a single region or country Crous et al. 2015;Linaldeddu et al. 2016;Yang et al. 2017;Dissanayake et al. 2021). Many Botryosphaeriaceae species have wide host ranges (e. g. ...
... guizhouensis have limited geographical distribution. So far, Barriopsis tectonae has been reported from China, Thailand and South Africa (Doilom et al. 2014;Dissanayake et al. 2021). Sardiniella guizhouensis and Sphaeropsis guizhouensis were only found in China while Sp. ...
... Multiple molecular systematic studies, mainly of pathogenic fungi of woody plants Slippers et al. 2013;Dissanayake et al. 2021;Zhang et al. 2021), have generated a robust phylogeny for Botryosphaeriaceae. However, the classification and identification of some species in this family remains a major challenge, due to the reasons 1) With the increase of the number of Botryosphaeriaceae species, morphological feature of inter-genera and inter-species is vague, 2) Some species occurred as asexual morph on nature and it is difficult to establish the link of asexual and sexual morph, 3) In general, Botryosphaeriaceae species do not show an obvious host specialization, while some populations displayed a certain degree of host association. ...
Woody oil plants are important economic trees which are widely cultivated and distributed throughout China. Surveys conducted during 2020 and 2021 on several woody oil plantations from five regions of Sichuan Province, China, revealed a high diversity of Botryosphaerialean fungi. The identification of 50 botryosphaeriaceous isolates was carried out based on both morphology and multi-gene phylogenetic analysis of internal transcribed spacer region (ITS), translation elongation factor 1-alpha gene ( tef1 ) and β-tubulin gene ( tub2 ). This allowed the identification of twelve previously known Botryosphaeriales species: Aplosporella prunicola , A. ginkgonis , Barriopsis tectonae , Botryosphaeria dothidea , Bo. fabicerciana , Diplodia mutila , Di. seriata , Dothiorella sarmentorum , Neofusicoccum parvum , Sardiniella guizhouensis , Sphaeropsis citrigena , and Sp. guizhouensis , and four novel species belonging to the genera Diplodia and Dothiorella , viz. Di. acerigena , Di. pistaciicola , Do. camelliae and Do. zanthoxyli . The dominant species isolated across the surveyed regions were Botryosphaeria dothidea , Sardiniella guizhouensis and Diplodia mutila , representing 20%, 14% and 12% of the total isolates, respectively. In addition, most isolates were obtained from Pistacia chinensis (14 isolates), followed by Camellia oleifera (10 isolates). The present study enhances the understanding of Botryosphaeriales species diversity on woody oil plants in Sichuan Province, China.
... Burgess et al. [31] and Garcia et al. [32] included 24 genera in Botryosphaeriaceae based on morpho-molecular data. However, Dissanayake et al. [33] mentioned that this family consists of 22 genera. This is the largest family in Botryosphaeriales [8,34]. ...
... Wikee et al. [3] reinstated Phyllostictaceae as a separate family in Botryosphaeriales to accommodate Phyllosticta, which consists of Phyllosticta and Pseudofusicoccum [34]. Phyllosticta species are mostly endophytes, but several are plant pathogens that cause leaf spots in a broad range of hosts worldwide [38][39][40][41][42]. Barr [43] introduced Planistromellaceae, which currently comprises two genera, namely, Kellermania and Umthunziomyces [33]. Saccharataceae is another family in Botryosphaeriales introduced by Slippers et al. [4] and consists of Pileospora, Saccharata and Septorioides [33]. ...
... Phyllosticta species are mostly endophytes, but several are plant pathogens that cause leaf spots in a broad range of hosts worldwide [38][39][40][41][42]. Barr [43] introduced Planistromellaceae, which currently comprises two genera, namely, Kellermania and Umthunziomyces [33]. Saccharataceae is another family in Botryosphaeriales introduced by Slippers et al. [4] and consists of Pileospora, Saccharata and Septorioides [33]. Ascospores and conidia in Botryosphaeriales have a wide range of morphologies, such as pigmented or hyaline, septate or aseptate and the presence or absence of a mucilaginous sheath (Table 1, Figure 2). ...
Botryosphaeriales (Dothideomycetes, Ascomycota) occur in a wide range of habitats as endo-phytes, saprobes, and pathogens. The order Botryosphaeriales has not been subjected to evaluation since 2019 by Phillips and co-authors using phylogenetic and evolutionary analyses. Subsequently, many studies introduced novel taxa into the order and revised several families separately. In addition , no ancestral character studies have been conducted for this order. Therefore, in this study, we re-evaluated the character evolution and taxonomic placements of Botryosphaeriales species based on ancestral character evolution, divergence time estimation, and phylogenetic relationships, including all the novel taxa that have been introduced so far. Maximum likelihood, maximum parsimony, and Bayesian inference analyses were conducted on a combined LSU and ITS sequence alignment. Ancestral state reconstruction was carried out for conidial colour, septation, and nutritional mode. Divergence times estimates revealed that Botryosphaeriales originated around 109 Mya in the early epoch of the Cretaceous period. All six families in Botryosphaeriales evolved in the late epoch of the Cretaceous period (66-100 Mya), during which Angiosperms also appeared, rapidly diversified and became dominant on land. Families of Botryosphaeriales diversified during the Paleogene and Neogene periods in the Cenozoic era. The order comprises the families Aplosporellaceae, Botryosphaeriaceae, Melanopsaceae, Phyllostictaceae, Planistromellaceae and Saccharataceae. Furthermore, current study assessed two hypotheses; the first one being "All Botryosphaeriales species originated as endophytes and then switched into saprobes when their hosts died or into pathogens when their hosts were under stress"; the second hypothesis states that "There is a link between the conidial colour and nutritional mode in botryosphaerialean taxa". Ancestral state reconstruction and nutritional mode analyses revealed a pathogenic/saprobic nutritional mode as the ancestral character. However, we could not provide strong evidence for the first hypothesis mainly due to the significantly low number of studies reporting the endophytic botryosphaerialean taxa. Results also showed that hyaline and aseptate conidia were ancestral characters in Botryosphaeriales and supported the relationship between conidial pigmentation and the pathogenicity of Botryosphaeriales species.
... The tree is rooted to Barriopsis stevensiana (CBS 174.26) and B. tectonae (MFLUCC 12-0381). Tree topology is similar to previous study conducted by Dissanayake et al. (2021). Bootstrap support values for ML ≥ 70% and Bayesian posterior probabilities (PP) ≥ 0.90 are noted at the nodes. ...
... Asexual morph: See Xiao et al. (2021). Known hosts and distribution:-Citrus unshiu in China (Xiao et al. 2021), decaying woody hosts in China (Dissanayake et al. 2021), Cinnamomum camphora in China (this study). ...
... Two Sphaeropsis and one Neodeightonia species were synonymized. Sphaeropsis guizhouensis collected from a decaying woody host in Guizhou province in China was introduced as a novel species based on morph-molecular data (Dissanayake et al. 2021). The phylogenetic analyses were performed using ITS, LSU and tef1-α sequences data. ...
Botryosphaeriaceae is the largest family in Botryosphaeriales, consisting of 22 genera. The family is worldwide in distribution and occurs on a wide variety of plant hosts. Botryosphaeriaceae includes endophytic, saprobic, and plant pathogenic species associated with canker and die-back diseases, most common on woody plants. The present study establishes two novel species of Dothiorella and new host and geographical records for species of Botryosphaeria, Dothiorella, Neodeightonia,
and Sphaeropsis. Taxa were identified based on morphology coupled with phylogenetic analyses based on maximum likelihood and Bayesian analyses of SSU, LSU, ITS, tef1-α and β-tub sequences. The taxa are described and illustrated.
The Botryosphaeriaceae is the largest family in Botryosphaeriales and currently comprises 22 genera of important endophytes, saprobes and plant pathogens. Most botryosphaeriaceous species have a cosmopolitan distribution, occurring on a wide range of woody hosts. Nonetheless, in many hosts, including palms (Arecaceae), the complex of associated Botryosphaeriaceae taxa is as yet unknown. The present study aimed to identify the botryosphaeriaceous species associated with foliar lesions of ornamental palms in Lisbon, Portugal. Twenty-nine Botryosphaeriaceae taxa were isolated from seven different palm species and identified based on both morphological and phylogenetic analyses. Six genera were detected: Botryosphaeria, Diplodia, Dothiorella, Neodeightonia, Neofusicoccum and Sardiniella. A new species of Neodeightonia, N. chamaeropicola, is introduced. Three botryosphaeriaceous species are reduced to synonymy. Thirteen new plant host-fungus associations are reported, while four new geographical records are noted for Portugal. A synopsis of accepted and phylogenetically validated Botryosphaeriaceae taxa reported from palms worldwide is presented. A total of 31 botryosphaeriaceous species have been currently reported from Arecaceae hosts, and many of them are associated with disease symptoms. This illustrates that more systematic studies are needed to examine the complex of Botryosphaeriaceae taxa associated with palms and determine their potential pathogenicity.
This article is the 14th in the Fungal Diversity Notes series, wherein we report 98 taxa distributed in two phyla, seven classes, 26 orders and 50 families which are described and illustrated. Taxa in this study were collected from Australia, Brazil, Burkina Faso, Chile, China, Cyprus, Egypt, France, French Guiana, India, Indonesia, Italy, Laos, Mexico, Russia, Sri Lanka, Thailand, and Vietnam. There are 59 new taxa, 39 new hosts and new geographical distributions with one new combination. The 59 new species comprise Angustimassarina kunmingense, Asterina lopi, Asterina brigadeirensis, Bartalinia bidenticola, Bartalinia caryotae, Buellia pruinocalcarea, Coltricia insularis, Colletotrichum flexuosum, Colletotrichum thasutense, Coniochaeta caraganae, Coniothyrium yuccicola, Dematipyriforma aquatic, Dematipyriforma globispora, Dematipyriforma nilotica, Distoseptispora bambusicola, Fulvifomes jawadhuvensis, Fulvifomes malaiyanurensis, Fulvifomes thiruvannamalaiensis, Fusarium purpurea, Gerronema atrovirens, Gerronema flavum, Gerronema keralense, Gerronema kuruvense, Grammothele taiwanensis, Hongkongmyces changchunensis, Hypoxylon inaequale, Kirschsteiniothelia acutisporum, Kirschsteiniothelia crustaceum, Kirschsteiniothelia extensum, Kirschsteiniothelia septemseptatum, Kirschsteiniothelia spatiosum, Lecanora immersocalcarea, Lepiota subthailandica, Lindgomyces guizhouensis, Marthe asmius pallidoaurantiacus, Marasmius tangerinus, Neovaginatispora mangiferae, Pararamichloridium aquisubtropicum, Pestalotiopsis piraubensis, Phacidium chinaum, Phaeoisaria goiasensis, Phaeoseptum thailandicum, Pleurothecium aquisubtropicum, Pseudocercospora vernoniae, Pyrenophora verruculosa, Rhachomyces cruralis, Rhachomyces hyperommae, Rhachomyces magrinii, Rhachomyces platyprosophi, Rhizomarasmius cunninghamietorum, Skeletocutis cangshanensis, Skeletocutis subchrysella, Sporisorium anadelphiae-leptocomae, Tetraploa dashaoensis, Tomentella exiguelata, Tomentella fuscoaraneosa, Tricholomopsis lechatii, Vaginatispora flavispora and Wetmoreana blastidiocalcarea. The new combination is Torula sundara. The 39 new records on hosts and geographical distribution comprise Apiospora guiyangensis, Aplosporella artocarpi, Ascochyta medicaginicola, Astrocystis bambusicola, Athelia rolfsii, Bambusicola bambusae, Bipolaris luttrellii, Botryosphaeria dothidea, Chlorophyllum squamulosum, Colletotrichum aeschynomenes, Colletotrichum pandanicola, Coprinopsis cinerea, Corylicola italica, Curvularia alcornii, Curvularia senegalensis, Diaporthe foeniculina, Diaporthe longicolla, Diaporthe phaseolorum, Diatrypella quercina, Fusarium brachygibbosum, Helicoma aquaticum, Lepiota metulispora, Lepiota pongduadensis, Lepiota subvenenata, Melanconiella meridionalis, Monotosporella erecta, Nodulosphaeria digitalis, Palmiascoma gregariascomum, Periconia byssoides, Periconia cortaderiae, Pleopunctum ellipsoideum, Psilocybe keralensis, Scedosporium apiospermum, Scedosporium dehoogii, Scedosporium marina, Spegazzinia deightonii, Torula fici, Wiesneriomyces laurinus and Xylaria venosula. All these taxa are supported by morphological and multigene phylogenetic analyses. This article allows the researchers to publish fungal collections which are important for future studies. An updated, accurate and timely report of fungus-host and fungus-geography is important. We also provide an updated list of fungal taxa published in the previous fungal diversity notes. In this list, erroneous taxa and synonyms are marked and corrected accordingly.
The description of a new Mediterranean species, Coltricia insularis, is provided, on the basis of material collected in Corsica, Sardinia, Cyprus and Spain
