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The influence of ischemia and reperfusion duration on left ventricular depolarization is studied from electrograms (ECG) and monophasic action potentials (MAPs) recorded by optical method. Left ventricular depolarization is evaluated by activation time (AT), which is defined as the distance from the earliest QRS deflection to the MAP upstroke and w...
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Citations
... influence of di-4-ANEPPS on electrical activity in isolated rabbit heart [8], course of global ischemia in isolated heart [2], study of various types of arrhythmias, evaluation of EG and MAP recorded during experiments with ischemia [2], [9], [10], study of relationship between EG and MAP [2], comparison of EG and MAP of isolated heart of rabbit and other species [8], comparison of EG and MAP of in vivo and isolated rabbit heart [11], [12], study of preconditioning in isolated rabbit heart [4], heart rate variability analysis [11], [12], evaluation of vectorcardiogram in control, ischemia, and reperfusion [13], testing of approaches for automatic delineation of EG and MAP, testing of approach for EG and MAP filtering, design and testing of mathematical methods for motion artefact suppression in MAP [14], design and testing of automatic classification of EG [15], T-wave alternans analysis, study of relationship between QT and RR intervals, etc. If the signals are used for evaluation of ischemia or preconditioning, the application of VSD and its possible effects on heart physiology must be taken into account to avoid the incorrect interpretation of the results. ...
During last decade, the myocardial ischemia was studied in our laboratory in isolated rabbit hearts. The three orthogonal electrograms and optical monophasic action potentials of the left ventricle were continuously recorded according to specific experimental protocol. The original data provided with comments are available in our database and may be useful for other researchers with various areas of interest.
Monophasic action potential (MAP) can be recorded from the heart surface by optical method based on fluorescence measurement. The motion of isolated heart during experiment caused additional noise in recorded signal. The motion artifact can be eliminated by ratiometric fluorescence emission measurements. This study is based on experiments in which optical MAP measurement is done by single-wavelength and dual-wavelength measurement of fluorescence emission. Both recording setups are presented and their advantages and disadvantages are discussed. MAPs recorded by both methods from isolated rabbit hearts perfused according to Langendorff are presented. Simultaneous electrograms (EG) and MAPs recording are analyzed and measurement of velocity of impulse conduction through heart tissue is presented.
