Figure 1 - available via license: Creative Commons Attribution 4.0 International
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An antero-posterior intra-cytoplasmic pH gradient in the tail bud and posterior PSM is regulated by glycolysis (A) Intra-cytoplasmic pH (pHi) in the posterior part of a 2-day old chicken embryo expressing pHluorin in the Tail Bud (TB) and Presomitic Mesoderm (PSM). Fluorescence intensity is shown by pseudocolor image (Fire color) using image J (right panel). Yellow signal indicates more acidic regions. Ventral view. NT: neural tube. Ant.: anterior, Post.: posterior. Scale bar, 100 μ m. (B) 488/405 nm ratio in individual cells in a representative embryo (n=3). Average values are calculated every 30 segmented cells along the AP axis. Error bars are S.D.
Source publication
Formation of the body of vertebrate embryos proceeds sequentially by posterior addition of tissues. While this process depends on aerobic glycolysis acting upstream of Wnt signaling in tail bud cells, the molecular details of this regulation are unknown. Here we used chicken embryos and human tail bud-like cells differentiated in vitro from iPS cel...
Context in source publication
Context 1
... reporter electroporated in vivo can report on intra- cytoplasmic pH changes. However, because it is impossible to perform the different steps required for calibration in the same embryo, different electroporated embryos were used for each pH value and for experimental measurements. Therefore, the absolute value of the pH could not be defined (Fig. 1A). Thus, for all in vivo measurements, we only compared the 405 to 488 nm fluorescence ...
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