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Amplication Profile of 15 rice genotypes with BF-S5 on Chromosome 6 for wide Compatibility Trait. Amplicons were Resolved on 1.5% Agarosegel Electrophoresis(AGE) Stained with Ethidium Bromide. Details of Genotypes: L=100bp Ladder, 1= IR58025B, 2= IR58025eB, 3=Dular, 4=Samba Mahsuri Sub1, 5=IR64Sub1, 6=BR11Sub1, 6=BR-11Sub1, 7=TDK-1Sub1, 8=CR-1009Sub1, 9=SwarnaSub1, 10=IR79156B, 11=BF-16B, 12=Oryza Meridionalis, 13=PRR78, 14=KMR3, 15=IR91-1591-3
Source publication
ABSTRACT Molecular breeding plays an important role in sustainable agriculture development. Hybrid rice technology aims to increase the yield potential of rice beyond the level of inbred high-yielding varieties (HYVs) by exploiting the phenomenon of hybrid vigour or heterosis. Improvement of hybrid rice parental line is necessary to meet the food s...
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Background: Chromosome segment substitution lines (CSSLs) can be used to dissect complex traits, from which single-segment substitution lines (SSSLs) containing a target quantitative trait loci (QTL) can be developed, and they are thus important for functional analysis and molecular breeding.
Results: A rice line with short wide grains, CSSL-Z563,...
Background
Chromosome segment substitution lines (CSSLs) can be used to dissect complex traits, from which single-segment substitution lines (SSSLs) containing a target quantitative trait loci (QTL) can be developed, and they are thus important for functional analysis and molecular breeding.
Results
A rice line with short wide grains, CSSL-Z563, w...
Bacterial blight (BB) of rice caused by the pathogen Xanthomonas oryzae pv. oryzae and the insect gall midge (GM) (Orseolia oryzae) are two major constraints of rice production. The present study was carried out to improve RPHR-1005, a stable restorer line of the fine-grain-type rice hybrid DRRH-3, for BB and GM resistance through marker-assisted b...
Citations
... The androgenic DH plants showed similar results for both SC3 and ART5 primers. This finding was consistent with previous reports (Sarkar and Bhattacharjee 2011;Priyadarshi et al. 2015) and indication of the presence of Sub-1A and Sub-1C alleles in the DH plants. Both Sub-1A and Sub-1C alleles are reported to have in Swarna Sub-1, IR64 Sub-1 and Samba Mahsuri Sub-1 (Sarkar and Bhattacharjee 2011). ...
The study was undertaken to explore the potential of doubled haploidy in fixation of dominant (submergence tolerance) and recessive (elongated uppermost internode) traits. Doubled haploids were produced by anther culture of F1 hybrids involving genotypes possessing sub1 and eui genes. The genotyping and phenotyping of traits were confirmed both at molecular and phenotypic levels. The molecular markers ART5 and SC3 were sufficient enough to screen for presence of Sub-1 gene, whereas RM 5970 could screen eui gene. Screening of DH lines for the targeted traits Sub-1 and eui, revealed the improvement of traits among DH lines. DH lines with submergence tolerant gene (Sub1) had significantly higher survival rate compared to susceptible checks, but on par with the Sub-1 parents. The tested genotypes exhibited considerable variation for eui trait. The significant variation for eui trait expression was observed among the DH lines. The degree of panicle exertion was on par with the donor parents and varied among the DH lines The study clearly indicates the scope for fixation of both dominant and recessive genes through doubled haploidy.
