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Amperometric experiments. A) Current traces measure at +500 mV using Accu-Check Performa glucose strips. 3 strips were used for each glucose sample. B) Average current between 4.8 and 5 seconds of the amperometric currents is plotted versus the glucose in the samples. Our device measured a linear relationship (R 2 = 0.98) over the physiological range of glucose within the range of the FDA's guidelines. https://doi.org/10.1371/journal.pone.0201353.g008
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Heavy metal ions (HMIs) are known to cause severe damages to the human body and ecological environment. And considering the current alarming situation, it is crucial to develop a rapid, sensitive, robust, economical and convenient method for their detection. Screen printed electrochemical technology contributes greatly to this task, and has achieved global attention. It enabled the mass transmission rate and demonstrated ability to control the chemical nature of the measure media. Besides, the technique offers advantages like linear output, quick response, high selectivity, sensitivity and stability along with low power requirement and high signal-to-noise ratio. Recently, the performance of SPEs has been improved employing the most effective and promising method of the incorporation of different nanomaterials into SPEs. Especially, in electrochemical sensors, the incorporation of nanomaterials has gained extensive attention for HMIs detection as it exhibits outstanding features like broad electrochemical window, large surface area, high conductivity, selectivity and stability. The present review focuses on the recent progress in the field of screen-printed electrochemical sensors for HMIs detection using nanomaterials. Different fabrication methods of SPEs and their utilization for real sample analysis of HMIs using various nanomaterials have been extensively discussed. Additionally, advancement made in this field is also discussed taking help of the recent literature.
The goal of achieving enhanced diagnosis and continuous monitoring of human health has led to a vibrant, dynamic and well-funded field of research in medical sensing and biosensor technologies. The field has many sub-disciplines which focus on different aspects of sensor science; engaging engineers, chemists, biochemists and clinicians, often in interdisciplinary teams. The trends which dominate include the efforts to develop effective point of care tests and implantable/wearable technologies for early diagnosis and continuous monitoring. This review will outline the current state of the art in a number of relevant fields, including device engineering, chemistry, nanoscience and biomolecular detection, and suggest how these advances might be employed to develop effective systems for measuring physiology, detecting infection and monitoring biomarker status in wild animals. Special consideration is also given to the emerging threat of antimicrobial resistance and in the light of the current SARS-CoV-2 outbreak, zoonotic infections. Both of these areas involve significant crossover between animal and human health and are therefore well placed to seed technological developments with applicability to both human and animal health and, more generally, the reviewed technologies have significant potential to find use in the measurement of physiology in wild animals.
This article is part of the theme issue ‘Measuring physiology in free-living animals (Part II)’.
