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7 Alternative splicing of the tra depending on Sxl Sxl binding to the 3' splice site of the red exon (containing a stop codon) can prevent inclusion of this exon in the tra transcript, removal of Sxl allows U2AF (a splicing component protein) to bind the 3' splice site of the red exon which leads to inclusion of the stop codon.
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A thorough and referenced account of the genetic engineering methods possible with CRISPR including advice for good experimental design.
Contents:
Chapter 1: Comparison of Genetic Engineering Methods
1.1 - Zinc Finger Proteins
1.2 - Tale Nucleases
1.3 - CRISPR System
Chapter 2: The Origin of CRISPR Technologies
2.1 - Three Major CRISPR Types
2.2 -...
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