FIGURE 4 - uploaded by A. Gregory Matera
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Alternative-splicing differences among snRNP biogenesis mutants. Distribution of significant alternative-splicing changes from small |delta Psi| near zero to large |delta Psi| near one: (A) Phax SH/SH mutants relative to WT; (B) Smn X7/D mutants relative to WT; and (C ) Ars2 C/E mutants relative to WT. (D) Venn diagram of overlapping alternative-splicing changes in snRNP biogenesis mutants, labeled as in Figure 2.
Source publication
Motor Neuron 1 gene (SMN1), but the mechanism whereby reduced levels of SMN protein lead to disease is unknown. SMN functions in the assembly of spliceosomal small nuclear ribonucleoproteins (snRNPs) and potential splicing defects have been uncovered in various animal models of SMA. We used disruptions in Smn and two additional snRNP biogenesis gen...
Context in source publication
Context 1
... the consideration of overlap- ping differences in Figure 3D. The overall trend of shorter transcripts in the mutants does not appear to be a conse- quence of developmental delay, as parallel DaPars analyses of modENCODE data sets of second to third instar larvae showed a trend toward shorter transcripts in the more ma- ture animals (Supplemental Fig. S4). Among the few overlap- ping mRNA length changes between Phax and Smn, one of the largest and most apparent changes was a shortened mRNA for CG9662, a putative oligosaccharyl transferase complex subunit (ΔPDUI = −0.96, Supplemental Table S5). These results are generally consistent with the notion that reductions in U1 snRNP levels ...
