Adiponectin expression during adipose depot-specific preadipocyte differentiation and the effect of rosiglitazone. (a) The expression of adiponectin mRNA normalized to glyceraldehyde3-phosphate dehydrogenase (GAPDH) from preadipocytes isolated from superficial-subcutaneous adipose tissue (sSAT), deep-SAT (dSAT), and visceral adipose tissue (VAT) abdominal depots following 9 days untreated (U) in serum-free medium or with differentiation cocktails with the inclusion (DIR) or absence of rosiglitazone (DI). Adiponectin and GAPDH mRNA were analyzed by semiquantitative reverse transcriptase-PCR with specific primers as described in Methods and Procedures. Results were taken at 30 cycles when transcripts were within the linear range and are given in arbitrary units. (b) In the same lean subjects, equal volume of the CM from 3, 6, and 9 days of U, DI, and DIR treated sSAT, dSAT, and VAT preadipocytes were run on a 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis to investigate the secretion of adiponectin by western immunoblot. Results shown are a representative short exposure of sSAT preadipocytes under the different treatments.

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... preadipocytes showed a decrease in LPL mRNA expres- sion from sSAT through to VAT (Figure 3a). With differen- tiation, there was a universal increase in the expression of LPL mRNA in all three depots which was further amplified with rosiglitazone, whereas depot-specific differences in (Figure 4b). This observation was further supported with the inclusion of rosiglitazone where adiponectin secretion was predominantly observed in sSAT cells as early as 6 days, while being evident at 9 days in dSAT cells and VAT cells with an overnight exposure (Figure 4b; data not shown). ...

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... differen- tiation, there was a universal increase in the expression of LPL mRNA in all three depots which was further amplified with rosiglitazone, whereas depot-specific differences in (Figure 4b). This observation was further supported with the inclusion of rosiglitazone where adiponectin secretion was predominantly observed in sSAT cells as early as 6 days, while being evident at 9 days in dSAT cells and VAT cells with an overnight exposure (Figure 4b; data not shown). ...

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... levels of adiponectin are strongly correlated to insu- lin resistance and atheroschlerosis with adiponectin synthesis almost exclusive to adipocytes. In all three depots, differentia- tion coexisted with an increase in the expression of adiponec- tin which was further amplified in the presence of rosiglitazone (Figure 4a). Between depots, no depot-specific changes were observed. ...