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A photomicrographic picture of the left ventricle of azithromycin and VIT. C treated rat showing a restored arrangement of cardiac muscle fibers with narrow spaces in between(stars). Few myocytes represent degenerated nuclei with vacuolated cytoplasm(irregular arrow) some other cells have a small atrophic nucleus(arrowhead). Some congested dilated blood vessels (arrows) and little cellular infiltration were observed (curved arrow). (H&E X400).
Source publication
Background: Azithromycin is one of the most common drugs used in the protocol of treatment of pneumonia caused by COVID-19. But many researchers approved its toxicity on heart tissue. VIT C is an available and strong antioxidant that has a protective effect against many toxins and drugs. This study aimed to study the cardiotoxic effect of Azithromy...
Context in source publication
Context 1
... myocytes represented degenerated nuclei with vacuolated cytoplasm also some other cells have small atrophic nuclei. Some congested dilated blood vessels and little cellular infiltration also appeared (Fig.3). In the Masson trichome stain of this treated group the collagen fibers decreased with non-significant change from the control(p=0.8) ...
Citations
... Drug dosing also increases plasma levels of MDA and TNF, respectively [21]. The current findings were supported by earlier research that showed an increase in heart damage-related enzymes in rats given azithromycin, including MDA, IL6 and TNF indicators [22]. Recent studies have shown that treating COVID-19 patients with azithromycin, with or without hydroxychloroquine, significantly increases the risk of critical QTc prolongation. ...
Background: Adverse medication reactions affecting one or more organ systems can manifest as side effects of antibiotics. There is a possibility that some antibiotics will have adverse effects that are fatal. The present study aims to examine the ability of hydroxytyrosol (HT) to protect the liver, heart , and improvement of kidney function against the adverse effects of azithromycin.Methods: Thirty adult male rats were randomly divided into three equal groups of 10 animals each. The first group, designated negative control (NC), was the daily oral administration of normal saline. The second group (T1) was subject to daily oral administration of azithromycin (30 mg/kg). The last third group (T2) was administered azithromycin (30 mg/kg) with hydroxytyrosol (50 mg/kg). After two weeks the animals were sacrificed for serum collection, histopathological specimens, and drug docking evaluation using Auto Dock Vina.Result: The present study showed that score binding in 18gs Glutathione S-transferase was preferable in hydroxytyrosol with (-5.95 Kcal/mol), while vitamin E was in (-7.80 Kcal/mol) and (-6.16 Kcal/mol) for glutathione. On the other aspect, antioxidant coenzyme Q10 showed less binding with 18gs Glutathione S-transferase to recorded binding at (-4.9) with Root Mean Square Deviation (RMSD) (29.3, 30.3, 23.9 Kcal/mol) and (28.6) Kcal/mol, respectively. The histopathological result for T1 showed several histological effects in the liver, heart, and kidney, while in T2 group showed improvement in tissue architecture. Conclusion: The study concluded that hydroxytyrosol binding glutathione S-transferase could predict potent antioxidants near glutathione, might be responsible for scavenger free radicals, and protect the tissue against the toxic effects of azithromycin.Keywords: Histopathology; Rat; Hydroxytyrosol; Azithromycin; Glutathione S-transferase
... Vitiligo, a very common cutaneous pigmentation abnormality, is characterized by the development of distinct macular patches of depigmentation as a result of the death of epidermal melanocytes [1] Interferon gamma (IFN-γ)-induced protein 10, or chemokine (C-X-C motif) ligand 10 (CXCL10), is a cytokine that is produced by a variety of cells in response to IFN-γ expression, including monocytes, fibroblasts, and endothelial cells [2]. By modulating the expression levels of Bcl-2, Bax, Bcl-2 homologous antagonist killer (Bak), and cleaved caspase-3 differently, IFN-γ may activate the JAK1/STAT1 signalling pathway and cause death of human melanocytes [3][4] CXCL10 expression is increased in several autoimmune disorders, including Graves' disease, rheumatoid arthritis, and type 1 diabetes mellitus [5][6]. High CXCL10 expression has also been implicated in the pathogenesis of vitiligo in multiple studies [7]. ...
Objectives
Vitiligo is a relatively common skin disfiguring disorder that exhibits a fluctuating course between activity and stability, making monitoring and management challenging. Autoimmunity plays a crucial role in the pathogenesis of vitiligo. Numerous autoimmune disorders have been associated with both CD27 and chemokine (C-X-C motif) ligand 10 (CXCL10). However, trials evaluating their role in vitiligo are lacking in the Egyptian setting. We evaluated the circulating levels of these two biomarkers in patients with vitiligo and the possible correlation between their levels and disease activity.
Methods
This cross-sectional study included 70 patients with vitiligo and 20 healthy controls. The patients were clinically assessed and then divided into active and stable groups according to clinical signs of activity and Vitiligo Disease Activity Scores. The levels of CD27 and CXCL10 in the serum were assessed using an enzyme-linked immunosorbent assay in both the patients and the controls, then the Mann-Whitney and Kruskal Wallis tests were used to analyze the data.
Results
Active and stable vitiligo patients have significantly higher median serum CXCL10 (385.9, and 245.2 pg/ml) and CD27 (61.6, and 66.5 ng/ml) levels compared to the controls (193 pg/ml, and 52.5 ng/ml respectively), p˂0.05 for all. In vitiligo cases, although CXCL10 levels significantly increased with disease activity ( P < 0.001), CD27 levels were comparable between the two subgroups ( P =0.953). CXCL10 positively correlated with disease activity (r=0.887, P < 0.0001). CXCL10 had higher sensitivity and lower specificity (95.7% and 60% respectively) compared to CD27 (71.4% and 75%, respectively) for differentiating cases from controls.
Conclusion
There is a possibility that CXCL10 and CD27 are involved in the development and course of vitiligo.
... In addition, AZ administration increased the oxidative stress and inflammatory response, as signified by increased plasma malondialdehyde (MDA) and tumor necrosis factor-alpha (TNFα), respectively ( Mohamed and Kasse, 2018). El-Naeem et al., (2022) mentioned that azithromycin drug-induced cardiotoxicity should be used in limited cases. The toxic effects of AZ on the heart can be potentially reduced by treatment with vit.C. ...
