Figure 1 - uploaded by Jack J Jiang
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A photograph of the laryngoscope set up: (A) rigid laryngoscope, (B) portable light source, (C) lens (Karl Storz 593A/N), (D) SOLIGOR 2X to fit Pentax, (E) adaptor, (F) camera, (G) iPad.
Source publication
Objective:
To evaluate a cost-effective modified rigid laryngoscopy setup with a portable light source and high-resolution commercially available digital camera for use in smaller otolaryngology and family practice clinics.
Methods:
The modified setup was used to obtain images of the larynx using both a traditional light source and a portable li...
Context in source publication
Context 1
... images were displayed on a screen via wired or wireless connection. All the devices listed previously are connected as pictured in Figure 1. Dr Zhang, who is an otolaryngologist with 2 years of rigid laryngoscopy examination experience, performed an exam on 1 healthy subject at the Eye & ENT Hospital of Fudan University in Shanghai, China. ...
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Objectives: The study aims to present the face and content validation of the LEAPA instrument, proposing a systematization for static description of the laryngoscopic findings of the benign organic lesions of the vocal folds with emphasis on the use of key elements of the morphological description (location / L / , extension / E /, organic aspect /...
Citations
... The rabbit larynx was visualized with aid of a 0 rigid laryngoscope (Zhejiang Tiansong Medical Instrument Co., Ltd, Hangzhou, China) coupled to an apparatus consisting of a camera (Sony α7S; Sony, Tokyo, Japan), lens (Karl Storz 593-A/N and SOLI-GOR 2X to fit Pentax; Karl Storz, Tuttlingen, Germany), adaptor (transferring M42 to E mount), portable light source (Karl Storz 11301 D4, LED light; Karl Storz), and normal light source (Stryker X6000, cold light; Stryker, San Jose, CA). 24 Laryngeal photos were taken at each time point. ...
Objectives/Hypothesis
Cryotherapy has been shown to be a scarless treatment modality for dermal lesions; however, there are limited data addressing the effect of cryotherapy on vocal fold tissue. The aim of this study was to clarify the effectiveness of cryotherapy for prevention of postsurgical vocal fold scarring.
Study Design
Prospective animal study in rabbits.
Methods
The lamina propria of 20 rabbit vocal folds was bilaterally stripped, followed by randomized unilateral cryotherapy. Five larynges were harvested for real‐time polymerase chain reaction (RT‐PCR) analysis at 1 day, 3 days, and 7 days postinjury. The remaining five were harvested for histologic analysis at 3 months. Images of the healing phase were recorded by laryngoscopy. Analyses of RT‐PCR for cyclooxygenase (COX)‐2, interleukin (IL)‐6, collagen I, collagen III, matrix metallopeptidase 1 (MMP1), transforming growth factor β (TGFβ1), α smooth muscle actin (α‐SMA), and hyaluronan synthase 1 (HAS1) were completed. Histological samples were completed for collagen and hyaluronic acid analysis.
Results
RT‐PCR results revealed that higher expressions of HAS1 and MMP1 and lower expressions of COX‐2, IL‐6, collagen I, collagen III, TGFβ1, and α‐SMA were observed, and histological examination showed significantly increased hyaluronic acid, decreased deposition, and more organized configuration of collagen in injury with the cryotherapy cohort compared with the injury cohort.
Conclusions
Cryotherapy can inhibit the inflammatory reaction and simulate a fetal healing environment in extracellular matrix synthesis to regenerate vocal fold tissue with less fibrosis. Histological results showed that cryotherapy achieves a mature healing result with less scar, which tends to return to normal. In summary, the findings of this study suggest that administration of cryotherapy at the time of injury has the potential to minimize vocal fold scarring.
Level of Evidence
NA Laryngoscope, 2018
Objectives
Vocal fold (VF) scarring is the major cause of voice disorders. Cryotherapy is an effective anti-scarring therapy for skin lesions. The aim of this study was to explore the anti-scarring potential of cryotherapy in vocal folds.
Methods
The extracellular matrix (ECM) mRNA expression of cryotherapy on normal VF tissue and the histologic results of cryotherapy on vocal fold healing were studied. Fifteen rats were introduced cryotherapy on the normal VF bilaterally and were harvested for real-time polymerase chain reaction (RT-PCR) analysis for collagen I, collagen III, TGFβ1, decorin, fibronectin and HAS1 at 1 day, 3 days and 7 days. Ten rats were unilaterally injured by stripping lamina propria and immediately treated with or without cryotherapy and were harvested at 2 months for histological and immunohistochemical analysis.
Results
Regenerative effect of cryotherapy was validated of ECM gene expression. Histological and immunohistochemical analysis showed significantly increased hyaluronan, decreased collagen, and increased decorin deposition in injury-cryotherapy cohort compared with injury control cohort and normal control cohort.
Conclusions
Cryotherapy may provide an optimal environment for vocal fold tissue regeneration. The results of the present investigation suggest that cryotherapy has therapeutic potential in prevention and treatment of vocal fold scarring.
