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A measuring scale to record the circumference of air cell with the help of a piece of yarn (small arrow) (G) and the holes indicated by large arrow (H) are the trace of probable internal pipping by egg tooth during the 10th day of incubation. 

A measuring scale to record the circumference of air cell with the help of a piece of yarn (small arrow) (G) and the holes indicated by large arrow (H) are the trace of probable internal pipping by egg tooth during the 10th day of incubation. 

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The chicken (Gallus domesticus L.) eggshells not only protect the contents of the egg from the microbes and physical stress but also control the exchange of water and gases through pores during the extra-uterine growth and development of embryo. The number of pores in blunt end statistically differed (p<0.01) from that in the equatorial region and...

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Context 1
... counting of pores in each piece of the eggshell (tip of both ends and any unit randomly selected from the equatorial region) was done through light microscopic observations (Fig. 1). The air cell circumference was measured by a piece of yarn ( Fig. 2 F) with the help of a measuring scale (Fig. 3 G). ...
Context 2
... pipping (by egg tooth) on the air cell membrane of the embryo side was observed by small holes. It continued and followed by scratching in the inner shell membrane (i.e., air cell membrane) on the 19th day until external pipping which started on the 20th incubation day. The first appearance was just holes like structure on air cell membrane ( Fig. 3 H) and later as crack in the shell (Fig. 7 h). After the 20th day (21st day), the chick hatched through breaking of eggshell lining of the equatorial region. ...
Context 3
... the 10th day of incubation, the embryo possibly made several hole like structures in the air cell by egg tooth (Fig. 3H). The embryonic position at 14 days of incubation seemed to be attached with interior portion of the air cell membrane (Fig. 4a). As the incubation days proceeded, the distance between the CAM and air cell membrane gradually decreased ( Fig. 7 a-h). The pore density increased with the increase of incubation day in the peking duck eggs ...
Context 4
... and the 20th day of incubation, respectively (Rahman et al., 2014) where there was a scratch opened externally through eggshell in this species. In contrast, Meir & Ar (1996) reported that drilling holes into the air cells on days 15 to 22 of incubation increased hatchability of goose eggs. At the 10th day of incubation, traces of several holes (Fig. 3H) were found to be present on the membrane (inner side) of air cell in this study. A temporary 'egg tooth' on the dorsal tip of the upper beak (Rahman et al., 2014) developed at this incubation day that might enable the chick to 'pip', which might be an initiation of 'internal ...

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