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A line drawing showing the morphological characters of Tubeufia asiana. A. Asci. B. Ascospores. C. Setae of ascomata. Bars 5 10 mm.
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The teleomorph of Aquaphila albicans was discovered on submerged wood collected in Thailand. Its black, soft-textured, setose ascomata, bitunicate asci and hyaline to pale brown, multiseptate ascospores indicated an affinity to Tubeufiaceae (Dothideomycetes). After morphological or molecular comparisons with related species in Tubeufia, Acanthostig...
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Context 1
... in Tsui and Berbee (2006 . We also included additional LSU rDNA sequences of fungi generated from the AFTOL project as well as the latest determined sequences from GenBank (Kodsueb et al 2006). Helicoma isiola R.T. Moore (AY856890) and Cenococcum geophilum (AY112935) were chosen as outgroups ( Tsui et al 2006). ...
Context 2
... decided against describing Tubeufia asiana as a species in Acanthostigma de Not. because an isolate of the type species of Acanthostigma, A. perpusillum (University of Alberta Microfungus Collection and Herbarium 7237), clustered phylogenetically within Tubeufia (Tsui et al 2006). This suggested that Acanthostigma probably should be synonymized un- der Tubeufia. ...
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Citations
... The sexual morphs of Tubeufiaceae are characterized by superficial ascomata, a pseudoparaphysate hamathecium, bitunicate asci, and many septate, hyaline to pale brown cylindrical ascospores (Barr 1979(Barr , 1980Rossman 1987;Kirk et al. 2001;Boonmee et al. 2011Boonmee et al. , 2014Brahamanage et al. 2017;Chaiwan et al. 2017;Dai et al. 2017;Hyde et al. 2017;Jayasiri et al. 2017;Lu et al. 2017a, b, c;Liu et al. 2018;Phookamsak et al. 2018). The asexual morphs of Tubeufiaceae are hyphomycetous, mostly helicosporous, some are chlamydosporous and phragmosporous Tsui et al. , 2007Boonmee et al. 2011Boonmee et al. , 2014Rajeshkumar and Sharma 2013;Hyde et al. 2016aHyde et al. , 2017Brahamanage et al. 2017;Chaiwan et al. 2017;Doilom et al. 2017;Lu et al. 2017aLu et al. , b, c, 2018Luo et al. 2017;Wijayawardene et al. 2017a, b;Kuo and Goh 2018a, b;Liu et al. 2018;Rajeshkumar et al. 2018). ...
... In the family Tubeufiaceae, helicosporous asexual morphs are represented in several genera, viz. Acanthohelicosporium, Chlamydotubeufia, Helicangiospora, Helicoma, Helicomyces, Helicosporium, Helicotubeufia, Neocanthostigma, Neohelicomyces, Neohelicosporium and Tubeufia (Kodsueb et al. 2006;Tsui et al. , 2007Boonmee et al. 2011Boonmee et al. , 2014Hyde et al. 2016a;Brahamanage et al. 2017;Chaiwan et al. 2017;Doilom et al. 2017;Lu et al. 2017aLu et al. , b, c, 2018Luo et al. 2017;Liu et al. 2018). Their general morphologies are characterized by partly superficial colonies on decaying woody substrates, macronematous conidiophores, holoblastic, monoblastic and/or polyblastic conidiogenous cells, and helicoid, septate, hyaline to variously coloured, smooth-walled conidia with varying number of conidial coils (Kodsueb et al. 2006;Tsui et al. , 2007Boonmee et al. 2011Boonmee et al. , 2014Hyde et al. 2016a;Brahamanage et al. 2017;Chaiwan et al. 2017;Doilom et al. 2017;Lu et al. 2017aLu et al. , b, c, 2018Luo et al. 2017;Liu et al. 2018). ...
... Acanthohelicosporium, Chlamydotubeufia, Helicangiospora, Helicoma, Helicomyces, Helicosporium, Helicotubeufia, Neocanthostigma, Neohelicomyces, Neohelicosporium and Tubeufia (Kodsueb et al. 2006;Tsui et al. , 2007Boonmee et al. 2011Boonmee et al. , 2014Hyde et al. 2016a;Brahamanage et al. 2017;Chaiwan et al. 2017;Doilom et al. 2017;Lu et al. 2017aLu et al. , b, c, 2018Luo et al. 2017;Liu et al. 2018). Their general morphologies are characterized by partly superficial colonies on decaying woody substrates, macronematous conidiophores, holoblastic, monoblastic and/or polyblastic conidiogenous cells, and helicoid, septate, hyaline to variously coloured, smooth-walled conidia with varying number of conidial coils (Kodsueb et al. 2006;Tsui et al. , 2007Boonmee et al. 2011Boonmee et al. , 2014Hyde et al. 2016a;Brahamanage et al. 2017;Chaiwan et al. 2017;Doilom et al. 2017;Lu et al. 2017aLu et al. , b, c, 2018Luo et al. 2017;Liu et al. 2018). ...
This study deals with an extensive taxonomic reevaluation focusing on phylogenetic relationships and morphological characterization of Tubeufiales, especially those helicosporous hyphomycetes which are difficult to identify. Based on evidence from DNA sequence data and morphology, we introduce 13 new genera in the family Tubeufiaceae, viz. Acanthotubeufia, Dematiohelicoma, Dematiohelicomyces, Dematiohelicosporum, Dematiotubeufia, Helicoarctatus, Helicohyalinum, Helicotruncatum, Neochlamydotubeufia, Neohelicoma, Pleurohelicosporium, Pseudohelicomyces and Pseudohelicoon; transfer Chaetosphaerulina from Dothideomycetes genera incertae sedis, and Artocarpomyces and Helicodochium from Ascomycetes genera incertae sedis into Tubeufiaceae; introduce 52 new species, viz. Berkleasmium fusiforme, B. longisporum, Chlamydotubeufia cylindrica, Dematiohelicosporum guttulatum, Helicoarctatus aquaticus, Helicodochium aquaticum, Helicohyalinum infundibulum, Helicoma aquaticum, H. brunneisporum, H. cocois, H. rufum, H. fusiforme, H. longisporum, H. multiseptatum, H. rubriappendiculatum, H. septoconstrictum, H. tectonae, Helicomyces hyalosporus, Helicosporium aquaticum, H. flavisporum, H. setiferum, H. vesicarium, H. viridiflavum, Neochlamydotubeufia fusiformis, Neohelicomyces hyalosporus, Neohelicosporium acrogenisporum, N. astrictum, N. ellipsoideum, N. irregulare, N. krabiense, N. laxisporum, N. ovoideum, Pleurohelicosporium parvisporum, Pseudohelicomyces aquaticus, P. hyalosporus, Tubeufia abundata, T. bambusicola, T. brevis, T. brunnea, T. chlamydospora, T. dictyospora, T. eccentrica, T. fangchengensis, T. hechiensis, T. inaequalis, T. krabiensis, T. rubra, T. sessilis, T. sympodihylospora, T. sympodilaxispora, T. taiwanensis and T. tratensis; provide 43 new combinations, viz. Acanthohelicospora guianensis, Acanthotubeufia filiforme, Berkleasmium aquatica, B. guangxiense, B. latisporum, B. thailandicum, Dematiohelicoma perelegans, D. pulchrum, Dematiohelicomyces helicosporus, Dematiotubeufia chiangraiensis, Helicohyalinum aquaticum, Helicoma elinorae, H. gigasporum, H. hongkongense, H. linderi, H. nematosporum, H. pannosum, H. serpentinum, Helicomyces chiayiensis, Helicotruncatum palmigenum, Neochlamydotubeufia khunkornensis, Neohelicoma fagacearum, Neohelicomyces pallidus, Neohelicosporium abuense, N. aurantiellum, N. griseum, N. morganii, N. myrtacearum, N. nizamabadense, N. sympodiophorum, N. taiwanense, N. vesiculiferum, Pseudohelicomyces indicus, P. paludosus, P. talbotii, Pseudohelicoon gigantisporum, P. subglobosum, Tubeufia dentophora, T. geniculata, T. lilliputea, T. machaerinae, T. sympodiophora and T. xylophila; introduce 16 new records, viz. Dictyospora thailandica, Helicomyces colligatus, H. torquatus, Neohelicosporium guangxiense, N. hyalosporum, N. parvisporum, Thaxteriellopsis lignicola, Tubeufia aquatica, T. chiangmaiensis, T. cylindrothecia, T. filiformis, T. guangxiensis, T. laxispora, T. parvispora, T. roseohelicospora and T. tectonae. The taxonomy of Helicoma, Helicomyces and Helicosporium is revisited based on phylogenetic analyses and morphological evidence. Neorhamphoria is transferred to Bezerromycetaceae. Three species are excluded from the genus Chlamydotubeufia, twelve species from Helicoma, four species from Helicomyces, 25 species from Helicosporium, six species from Neoacanthostigma and one species from Tubeufia. A multi-gene phylogenetic tree based on maximum likelihood and Bayesian analyses of ITS, LSU, RPB2 and TEF1α sequence data of species of Tubeufiales is provided. Detailed descriptions and illustrations are provided, as well as the morphological comparison with similar taxa are explored. The checklist of accepted Tubeufiales species and re-organised Tubeufiales species are provided.
... RAxML, MP, and Bayesian analysis of the combined dataset resulted in phylogenetic reconstructions with largely similar topologies, and the Bayesian tree is shown in Fig. 1. Representatives of the sequenced genera (with molecular data) of Tubeufiaceae (Tsui et al. 2006(Tsui et al. , 2007 Tsui and Berbee Boonmee et al. 2011Boonmee et al. , 2014Rajeshkumar and Sharma 2013;Doilom et al. 2017;Luo et al. 2017;Brahmanage et al. 2017;Phookamsak et al. 2017) are included in our phylogenetic analysis (Fig. 1). Twenty genera are represented by at least one species in Tubeufiaceae. ...
Helicosporous hyphomycetes are a morphologically allied group of Tubeufiales. We introduce a new helicosporous genus, Neohelicosporium, with five new species, Neohelicosporium aquaticum, N. guangxiense, N. hyalosporum, N. parvisporum, and N. thailandicum, based on morphological and phylogenetic evidence. The RPB2 protein gene data are provided to analyze their phylogeny in Tubeufiales. Phylogenetic analyses of combined ITS, LSU, RPB2, and TEF1α sequence data from 13 new isolates of Neohelicosporium provided evidence to support the establishment of the new taxa. The morphological characters of Neohelicosporium that differentiate it from other helicosporous species are compared and discussed.
... Representatives of the sequenced genera (with molecular data) of Tubeufiaceae (Tsui et al. 2006(Tsui et al. , 2007Tsui &B erbee 2006;Promputtha &M iller 2010;Boonmee et al. 2011Boonmee et al. , 2014Rajeshkumar &S harma 2013;Hyde et al. 2016;Doilom et al. 2017;Lu et al. 2017aLu et al. , 2017bLuo et al. 2017) are includedi no ur phylogenetic analysis (Fig. 1). Sixteen genera are represented by at least one species in Tubeufiaceae. ...
Helicosporous hyphomycetes are a morphologically fascinating group of Tubeufiales. Ten dematiaceous helicosporous asexual morphs and two sexual morphs collected from aquatic habitats are characterized in this study using morphological features and phylogenetic analyses. Four new species of Neoacanthostigma, viz. N. aquaticum, N. brunneisporum, N. guangxiense and N. latisporum, are described and illustrated. The phylogenetic analyses of combined ITS, LSU and TEF1α sequence data place all taxa in the genus Neoacanthostigma (Tubeufiaceae, Tubeufiales) and provide evidence to support the establishment of the new taxa. The differentiating morphological characters of Neoacanthostigma with other helicosporous species are compared and discussed. A key to all Neoacanthostigma species is also provided.
... Currently,t he genus Tubeufia comprises 27 species,1 4s pecies whose placement are confirmed with molecular data, and to date nine species are reported from freshwater habitats (Lu et al. 2017). Some Tubeufia species reproduce asexually in culture and have been connected to asexual genera, such as Aquaphila, Helicoma, Helicomyces or Helicosporium.M ost of these established links have been confirmed by cultural methods and supported by DNA sequence data (Tsui et al. 2006(Tsui et al. , 2007Promputtha &M iller 2010;Sánchez &B ianchinotti 2010;Sánchez et al. 2012;Boonmee et al. 2014;Lu et al. 2017). The genus Helicomyces established by Link (1809) with H. roseus as type species, is characterized by helicoid, tightly or loosely coiled, multi-septate, filamentous, hyaline, white, pinkish and brown conidia (Link 1809;Boonmee et al. 2014). ...
... Sequence data for relevant strains were downloaded from GenBank following data from recent publications (Kodsueb et al 2006;Schoch et al. 2006;Tsui et al. 2006Tsui et al. , 2007Promputtha &M iller 2010;Boonmee et al. 2011Boonmee et al. , 2014Sánchez et al. 2012;Slippers et al. 2013;Suetrong et al. 2014;Doilom et al. 2016;Hyde et al. 2016a;Lu et al. 2017). Raw sequences were assembled with Sequencher 4.9 for Windows (Gene Codes Corp., Ann Arbor,M ichigan). ...
The diversity of lignicolous freshwater fungi along a north-south latitudinal gradient are currently being studied in Asia. In this paper, we report on 18 collections of asexual morphs of Tubeufiaceae from submerged wood in rivers, streams and a lake in Yunnan Province, China. Taxa are characterized based on morphological characters and analyses of ITS, LSU and TEF1α sequence data. The new genera, Muripulchra with a single species, M. aquatica and Neohelicomyces with three new taxa (N. aquaticus, N. grandisporus, N. submersus) are introduced. Muripulchra is characterized by micronematous conidiophores and obpyriform, septate to muriform conidia. Neohelicomyces is characterized macronematous conidiophores and multi-septate, helicoid conidia. Tubeufia aquatica is introduced as a new species and its phylogenetic relationships with other taxa is discussed. The phylogenetic analyses of a concatenated ITS, LSU and TEF1α dataset place all collections in the family Tubeufiaceae (Tubeufiales) and provide evidence to support the establishment of our new taxa. The asexual morph of Tubeufia cylindrothecia, the type species of Helicomyces (H. roseus) are described herein, phylogenetic relationships assessed and reference specimens are given for these two species. Descriptions and illustrations for the new genera and species are provided with notes on their taxonomy and phylogeny.
... Notes: Clade J represents Aquaphila and comprises species with darkly pigmented ascomata covered by dark setae, cylindrical-clavate and apically thickened asci, and fusiform 6-7-septate ascospores. The asexual form occurs in an aquatic habitat and produces curved conidia that are distinct from the type species in the sister genus Chlamydotubeufia, C. huaikangplaensis that produces dictyochlamydospores (Tsui et al. 2007;Boonmee et al. 2011 B i f r o n t i a N or m a n , B o t . N o t i s e r : 18 ( 18 7 2) , Facesoffunginumber: FoF 00208 ...
... Dictyochlamydospores broadly oblong, elongate, multiseptate, at first reddish-brown, becoming black (from Boonmee et al. 2011 Chlamydotubeufia (Clade I) includes terrestrial taxa and produces dictyochlamydospores. This genus is distinct from the aquatic genus Aquaphila (Clade J) which produces sickleshaped conidia (Tsui et al. 2007;Boonmee et al. 2011 Notes: Phylogenetically Helicangiospora lignicola does not group with any other taxa and is basal to Clades A-B, although lacks support. The genus is also morphologically distinct as it produces a unique helicoma-like asexual state in culture (Fig. 13). ...
... Illustrations presented here are based on BPI 447569 considered authentic material of H. muelleri on natural substrate and determined by D. Linder. Various Helicoma species have been connected with sexual taxa in Tubeufiaceae and the links are confused (Tsui et al. , 2007Zhao et al. 2007;Boonmee et al. 2011 Conidiophores (43-)80-153.5 μm long × 7-10 μm wide (x¼ 106 Â 8μm , n = 20), superficial, macronematous, crowded, erect, dark brown, septate, rarely branched, darkened at septum. Conidiogenous cells monoblastic to polyblastic, sometimes branched at apex, brown to dark brown, smooth-walled. ...
Abstract Tubeufiaceae is based on the generic type Tubeufia,
which is characterized by superficial, oval and bright ascomata,
bitunicate asci, mostly long fusiform to filiform, transeptate
ascospores and hyphomycetous asexual states with
helicosporous conidia. Most species in this family are saprobic
on terrestrial woody substrates and some are aquatic. Their
distinct morphology as well as combined LSU, SSU and
TEF1 sequence analysis show that Tubeufiaceae should be
accommodated in a new order Tubeufiales, which is introduced
in this paper. Phylogenetic analyses of combined LSU and ITS
sequences were used to resolve genera and species within the
family Tubeufiaceae. In this study, we examine and incorporate
sexual and asexual states of genera in Tubeufiales to provide a
modern treatment, based on single names. An epitype for
Tubeufia javanica, the type species of Tubeufia, is designated
and represents Tubeufia sensu stricto. The genera
Acanthophiobolus, Acanthostigma, Boerlagiomyces,
Chlamydotubeufia, Kamalomyces, Podonectria, Thaxteriella
and Thaxteriellopsis are accepted, Acanthostigmina is reinstated,
and the asexual genera Aquaphila, Helicoma, Helicomyces,
Helicosporium and Tamhinispora are accepted in Tubeufiaceae.
Three new genera Acanthohelicospora, Helicangiospora and
Neoacanthostigma are introduced. The genus Bifrontia is added
to the family based on morphological similarity. The incongruous
morphological genera Acanthostigmella, Amphinectria,
Chaetocrea, Chaetosphaerulina, Glaxoa, Malacaria,
Melioliphila, Paranectriella, Puttemansia, Rebentischia and
Uredinophila are excluded from Tubeufiaceae despite having
characteristic ascomata with setae and multiseptate long spores.
A key to genera accepted in Tubeufiaceae is provided.
Keywords Dothideomycetes . Helicospores . New genus .
Phylogeny . Taxonomy . Tubeufiaceae
... Zhao et al. (2007), in their study on helicosporous hyphomycetes from China, observed the same teleomorphic-anamorphic connection between T. cylindrothecia and Helicomyces roseus. In the cladogram presented by Tsui et al. (2007), however, T. cylindrothecia and Helicomyces roseus appeared in several separated clades. On the other hand, analysis of ribosomal SSU, ITS and partial LSU data has shown that Tubeufia sensu Barr and most of the helicosporic anamorphs were close relatives, clustering with 100% bootstrap support in a clade designated 'Tubeufiaceae s. str.' (Tsui and Berbee 2006). ...
The Yungas of northwestern Argentina are located at the southern limit of a system of tropical and subtropical cloud montane forests extending on the oriental slope along the Andes in South America. Although they cover only less than 2% of the whole Argentinian surface, these mountain forests harbor over 50% of its biodiversity (flora and fauna) (Brown et al. 2005). All mycological investigations carried out in this region () indicate that the fungal diversity of this region is still very poorly known. So far, 755 fungal species have been reported from the southern limit of the Yungas forests in the Tucumán province (Hladki et al. unpublished data). We started an exhaustive survey of two protected areas of the Yungas and their surroundings, the Reserva Experimental Horco Abstract: We report new records for nine species of ascomycetes from the Yungas of northwestern Argentina. Capronia nigerrima, Capronia pulcherrima and Rosellinia dingleyae are new records for South America. Byssosphaeria rhodomphala, Camarops tubulina, Cercophora ambigua, Chaetosphaerella phaeostroma, Tubeufia cylindrothecia are new species for Argentina, and Togninia minima is a new record for the biome.
... In addition, numerous D1/D2 sequences, corresponding to dif-ferent classes, orders and families of ascomycetes retrieved from GenBank or NITE/NRBC databases were included in the phylogenetic study (Table 1). Most of these sequences were published by different authors (Sugiyama et al. 1999, Sugiyama & Mikawa 2001, Untereiner et al. 2002, Reeb et al. 2004, Xi et al. 2004, Murata et al. 2005, Wang et al. 2005, Wedin et al. 2005, Kodsueb et al. 2006, Réblová & Seifert 2007, Tsui et al. 2007, Gueidan et al. 2008, Boehm et al. 2009, Sugiyama et al. 2002, Boonmee et al. 2011, Pettersson et al. 2011, Réblová et al. 2011, Giraldo et al. 2013. The selection of these sequences was based on the results of a BLAST search using the D1/D2 and ITS sequences from each of the ex-type strains of the different species of Arthrographis and Arthropsis. ...
Numerous members of Ascomycota and Basidiomycota produce only poorly differentiated arthroconidial
asexual morphs in culture. These arthroconidial fungi are grouped in genera where the asexual-sexual connections and their taxonomic circumscription are poorly known. In the present study we explored the phylogenetic relationships of two of these ascomycetous genera, Arthrographis and Arthropsis. Analysis of D1/D2 sequences of all species of both genera revealed that both are polyphyletic, with species being accommodated in different orders and classes. Because genetic variability was detected among reference strains and fresh isolates resembling the genus Arthrographis, we carried out a detailed phenotypic and phylogenetic analysis based on sequence data of the ITS region, actin and chitin synthase genes. Based on these results, four new species are recognised, namely Arthrographis chlamydospora, A. curvata, A. globosa and A. longispora. Arthrographis chlamydospora is distinguished by its cerebriform colonies, branched conidiophores, cuboid arthroconidia and terminal or intercalary globose to subglobose chlamydospores. Arthrographis curvata produced both sexual and asexual morphs, and is characterised by navicular ascospores and dimorphic conidia, namely cylindrical arthroconidia and curved, cashew-nut-shaped conidia formed laterally on vegetative hyphae. Arthrographis globosa produced membranous colonies, but is mainly characterised by doliiform to globose arthroconidia. Arthrographis longispora also produces membranous colonies, but has poorly differentiated conidiophores and long arthroconidia. Morphological variants
are described for A. kalrae and our results also revealed that Eremomyces langeronii and A. kalrae, traditionally considered the sexual and asexual morphs of the same species, are not conspecific.
... Tsui et al. (2006) assessed their phylogenetic relationships from ribosomal sequences of ITS, 5.8S and partial LSU regions, and found that 45 isolates from the three genera were closely related and were within the sexual genus Tubeufia (Tubeufiaceae). The freshwater hyphomycete Aquaphila albicans, reported on submerged wood, was also assigned to Tubeufiaceae (Tsui et al. 2007;Shearer et al. 2009). More recently Boonmee et al. (2011) have undertaken a revision of the sexual stages assigned to the Tubeufiaceae and included a number of asexual species as well. ...
... Most taxa of Tubeufiaceae are commonly found on woody litter, although some species can also be found on leaf litter or even decaying cloth and some are associated with other fungi or scale insects (Barr 1980;Rossman 1987;Kodsueb et al. 2006;Promputtha and Miller 2010;Sánchez and Bianchinotti 2010). The anamorphs of Tubeufiaceae have been well-studied and are mostly related to helicosporous taxa such as Helicoma, Helicomyces and Helicosporium (Tsui et al. , 2007Hyde et al. 2011). ...
... nov. to belong in Tubeufia.The anamorphs of Tubeufia are recorded as mostly helicosporous. Aquaphila is tubeufiaceous but did not cluster with the Tubeufia paludosa clade in the phylogenetic analyses (Tsui et al. , 2007Promputtha and Miller 2010) and appears to be a distinct genus. Helicoma and Helicosporium are shown to be polyphyletic in this and other studies (Tsui et al. , 2007Promputtha and Miller 2010; Fig. 1). ...
... Aquaphila is tubeufiaceous but did not cluster with the Tubeufia paludosa clade in the phylogenetic analyses (Tsui et al. , 2007Promputtha and Miller 2010) and appears to be a distinct genus. Helicoma and Helicosporium are shown to be polyphyletic in this and other studies (Tsui et al. , 2007Promputtha and Miller 2010; Fig. 1). The anamorphs of the genera Tubeufia will need to be reconsidered following this study. ...
In this paper we revisit the family Tubeufiaceae with notes on genera that we have re-examined where possible. Generic type specimens of Acanthophiobolus, Kamalomyces, Podonectria, Thaxteriella and Thaxteriellopsis were re-examined, described and illustrated and shown to belong to Tubeufiaceae. Notes are provided on Acanthostigma, Chaetosphaerulina, Thaxterina and Tubeufia, which are retained in Tubeufiaceae; however, we were unable to locate the types of these genera during the time frame of this study. Allonecte is excluded from the Tubeufiaceae, as the ascospores are fusiform-ellipsoidal, grey-brown and 1-septate and the asci are cylindrical, all of which are features more typical of Pleosporaceae, where it is transferred. Byssocallis has yellow to orange ascomata and clavate ascospores which is atypical of Tubeufiaceae. Thus its taxonomic status needs to be reevaluated. Lentendraeopsis has an endophytic habit, cylindro-clavate asci and two-celled ascospores more typical of Pleosporales, where it is transferred. Taphrophila has small ascomata, a thin peridium, branching setae around the apex of the ascomata, clavate to saccate asci and lacks pseudoparaphyses. These are features atypical of the Tubeufiaceae, and Taphrophila should be placed in the Dothideomycetes incertae cedis. Twelve new collections of Tubeufiaceae from Thailand were isolated, and their DNA was extracted. The sequence data of LSU, SSU and ITS rDNA were amplified and analyzed using parsimony and likelihood methods. The results of phylogenetic analysis was used to establish the inter-generic relationships in Tubeufiaceae. Thaxteriellopsis lignicola, epitypified in this investigation, is a sister taxon in the family Tubeufiaceae based on phylogenetic analysis of rRNA sequence data. Chlamydotubeufia is introduced as a new genus based on the production of dictyochlamydosporous anamorphs, including two new species. Three new species, one each in Acanthostigma, Tubeufia and Thaxteriella are also described and illustrated. The phylogenetic placement of these genera is also discussed.
... (Willoughby & Archer 1973), Clavariopsis aquatica to Massarina sp. (Webster & Descals 1979), and Aquaphila albicans to Tubeufia asiana (Tsui et al. 2007). Four connections are published on the basis of molecular phylogenetic rather than cultural studies, but some of these connections are controversial and require further molecular study using additional genes and/or cultural studies. ...
The freshwater Dothideomycetes species are an ecological rather than taxonomic group and comprise approximately 178 meiosporic and mitosporic species. Due to convergent or parallel morphological adaptations to aquatic habitats, it is difficult to determine phylogenetic relationships among freshwater taxa and among freshwater, marine and terrestrial taxa based solely on morphology. We conducted molecular sequence-based phylogenetic analyses using nuclear ribosomal sequences (SSU and/or LSU) for 84 isolates of described and undescribed freshwater Dothideomycetes and 85 additional taxa representative of the major orders and families of Dothideomycetes. Results indicated that this ecological group is not monophyletic and all the freshwater taxa, except three aeroaquatic Tubeufiaceae, occur in Pleosporomycetidae as opposed to Dothideomycetidae. Four clades comprised of only freshwater taxa were recovered. The largest of these is the Jahnulales clade consisting of 13 species, two of which are the anamorphs Brachiosphaera tropicalis and Xylomyces chlamydosporus. The second most speciose clade is the Lindgomycetaceae clade consisting of nine taxa including the anamorph Taeniolella typhoides. The Lindgomycetaceae clade consists of taxa formerly described in Massarina, Lophiostoma, and Massariosphaeriae.g.,Massarina ingoldiana, Lophiostoma breviappendiculatum, and Massariosphaeria typhicola and several newly described and undescribed taxa. The aquatic family Amniculicolaceae, including three species of Amniculicola, Semimassariosphaeria typhicola and the anamorph, Anguillospora longissima, was well supported. A fourth clade of freshwater species consisting of Tingoldiago graminicola,Lentithecium aquaticum,L. arundinaceum and undescribed taxon A-369-2b was not well supported with maximum likelihood bootstrap and Bayesian posterior probability. Eight freshwater taxa occurred along with terrestrial species in the Lophiostoma clades 1 and 2. Two taxa lacking statistical support for their placement with any taxa included in this study are considered singletons within Pleosporomycetidae. These singletons, Ocala scalariformis, and Lepidopterella palustris, are morphologically distinct from other taxa in Pleosporomycetidae. This study suggests that freshwater Dothideomycetes are related to terrestrial taxa and have adapted to freshwater habitats numerous times. In some cases (Jahnulales and Lindgomycetaceae), species radiation appears to have occurred. Additional collections and molecular study are required to further clarify the phylogeny of this interesting ecological group.
