Figure - available from: Euphytica
This content is subject to copyright. Terms and conditions apply.
A histogram of the relative fluorescence intensity of nuclei isolated from the fresh leaves of (peak 1) hexaploid haskap and an internal standard (peak 2) Capsicum annuum cv. ‘Kyonami’ analyzed by the flow cytometer
Source publication
The relationship between the ploidy level and phenotype in haskap (Lonicera caerulea L. subsp. edulis (Turcz. ex Herder) Hultén) is mostly unclear. Therefore, this study investigated the fruit and leaf phenotypes of diploid, tetraploid, pentaploid, hexaploid, and aneuploid (2n = 6x − 3) haskap plants. A concomitant positive increase in the ploidy l...
Citations
... Many previous studies have shown that polyploidization leads to considerable changes in the morphology, physiology, and biochemistry of plants and is a key driver of macro-evolutionary success [3][4][5][6]. Specifically, polyploidization has been shown to change plants' height [7][8][9], leaf index [10], fruit size [11,12], and secondary metabolite levels [13,14], among others. ...
Polyploidization produces abundant phenotypic variation. Little is currently known about adventitious root (AR) development variation due to polyploidization. In this study, we analyzed the morphological, cytological, and physiological variations in AR development between tetraploid and diploid Populus plants during in vitro rooting culture. Compared to the diploids, the AR formation times and rooting rates of the tetraploids’ stem explants had non-significant changes. However, the tetraploid ARs exhibited significantly slower elongation growth than the diploid ARs. Cytological observation showed that the tetraploid ARs were characterized by shorter root meristems and reduced meristem cell numbers, suggesting the reasons for the slow AR elongation. Analysis of hormones and related metabolites during AR development demonstrated that the total auxin, cytokinin, and jasmonic acid contents were significantly lower in the tetraploid ARs than in those of the diploids, and that the ratio of total auxins to total CKs at 0 h of AR development was also lower in the tetraploids than in the diploids, whereas the total salicylic acid content of the tetraploids was consistently higher than that of the diploids. qPCR analysis showed that the expression levels of several hormone signaling and cell division-related genes in the tetraploid ARs significantly differed from those in the diploids. In conclusion, the slow elongation of the tetraploid ARs may be caused by the endogenous hormone-mediated meristem shortening. Our findings enhance the understanding of polyploidization-induced variation in AR development of forest trees.
