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A. Wild-common guppy used in this research, B. Clumps of various ornamental guppies (Poecilia reticulata). (photo: from several sources)  

A. Wild-common guppy used in this research, B. Clumps of various ornamental guppies (Poecilia reticulata). (photo: from several sources)  

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Hedayati A, Tarkhani R, Shadi A. 2012. Mortality response of Poecilia reticulata Peters 1859 (Cyprinodontiformes: Poeciliidae) to some agricultural pesticides. Nusantara Bioscience 4: 6-10. This research was performed to determine and compare acute toxicity of diazinon and deltamethrin as potential dangerous organic pesticides to assess mortality e...

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... The fish were not fed anything throughout the experiment. Each fish weighed approximately 0.5 g [6,7,9,11]. The OECD 203 standard procedures [12] were followed for conducting the acute fish toxicity test. ...
... The fish behaviour was thoroughly observed, and any dead fish were removed immediately. After 24, 48, 72, and 96 hours, the number of dead fish at each concentration was counted [6,7,9,11]. The concentration required for the death of 50% of the test fish within 96 hours, or the median lethal concentration (LC 50 ), was subsequently determined. ...
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