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A Maximum Likelihood tree inferred from sequences of the ITS and 5.8S regions using RAxML (Stamatakis 2014). Bootstrap values >70% are indicated above or near branches. The Cambridge sample is the Pucciniastrum sp. shown in red.

A Maximum Likelihood tree inferred from sequences of the ITS and 5.8S regions using RAxML (Stamatakis 2014). Bootstrap values >70% are indicated above or near branches. The Cambridge sample is the Pucciniastrum sp. shown in red.

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Fig. 1. Pucciniastrum sp. on the lower surface of Soleirolia leaves,...
Fig. 2. Pucciniastrum sp. on lower surface of Soleirolia leaf,...
Fig. 3. Urediniospores (×600) of the Pucciniastrum sp. on Soleirolia....
Fig. 4. Fluorescent image of urediniospores showing binucleate nature...
Fig. 5. A Maximum Likelihood tree inferred from sequences of the ITS...
A newly discovered rust on Soleirolia soleirolii
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  • Apr 2023
A newly discovered rust on Soleirolia soleirolii in the UK
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... study by Liang et al. (2006) used the ITS1-5.8S-ITS2 region to evaluate the phylogenetic relationships of 14 morphologically similar species of Pucciniastrum found in Japan. A further extraction and amplification was therefore carried out on the Cambridge collection using the primers and conditions specified by Liang et al. The resultant tree (Fig. 5), which also includes a few sequences from GenBank, demonstrates that the Cambridge material is clearly distinct from five other British species included in the analysis, Naohidemyces vaccinii (N. vacciniorum), P. agrimoniae, P. circaeae, P. epilobii and Thekopsora areolata (P. areolatum), and it confirms its similarity to the group of ...