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A. Keissleriella linearis. Section of an ascoma, note the setae in ostiole. B-D. Lophiotrema nucula sensu Holm. B. Peridium section of an ascoma. Note the peridium in equal thickness. C, D. Habitate section of ascomata. Scale bars: A = 100 µm; B-D = 50 µm.
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Towards a phylogenetic clarification of Lophiostoma / Massarina and morphologically similar genera in the Pleosporales. Fungal Diversity 38: 225-251. Lophiostoma, Lophiotrema and Massarina are similar genera that are difficult to distinguish morphologically. In order to obtain a better understanding of these genera, lectotype material of the generi...
Citations
... The sexual morph of Bambusicolaceae is characterized by solitary or gregarious, immersed, semi-immersed to erumpent and conical or globose to subglobose ascomata; cylindrical to clavate asci with short furcate or rounded to obtuse pedicels; hyaline or yellowish to brown, slightly broad-fusiform or clavate to ellipsoidal, 1-to multi-septate ascospores and mostly with a gelatinous sheath (Dai et al. 2012, Hyde et al. 2013, Liu et al. 2015, Dong et al. 2020. The asexual morph of coelomycete Bambusicolaceae is characterized by cylindrical conidiogenous cells, and hyaline to brown, cylindrical to ellipsoidal conidia (Tanaka et al. 2009, Zhang et al. 2009, Dai et al. 2012, Hyde et al. 2013. ...
Macadamia integrifolia, one of the cash crops widely grown in the tropical and subtropical regions produces edible kernels with high oil content. Several fungal pathogens have been reported from this host, but very few studies on saprobic fungi have been carried out. As a part of our studies on saprobic fungi of edible nuts, dead branches of Macadamia integrifolia with fungal fruiting bodies were collected from Yunnan Province of China. Molecular analyses of combined SSU, ITS, LSU, RPB2, and TEF1-α sequence data showed that our collection belongs to Bambusicolaceae and forms a distinct branch with other genera in this family. Our collection fits well with the Bambusicolaceae, and morphology is similar to Palmiascoma but can be distinguished by molecular data. Therefore, we introduce our collection as Neopalmiascoma gen. nov., with N. macadamiae as the type species. A description, photo plate, and phylogenetic tree to show the taxonomic placement of the new species are provided.
... Type Known host and distribution: FRANCE, Ariège, Rimont, Peyrau, on submerged wood of Fraxinus excelsior; on submerged wood of Alnus glutinosa; Le Baup brook, along D 18, on submerged wood of Platanus sp. [35]. ...
... Notes: Lentithecium aquaticum was introduced by Zhang et al. [35] based on phylogenetic analysis and morphological characteristics. The placement of this species was not stable and has been changed by several studies [10,45,71,74]. ...
... Comparison of ITS, LSU, SSU, and tef 1-α sequences between Paralentithecium suae and P. aquaticum revealed 11 bp, 4 bp, 4 bp, and 22 bp differences, respectively. Paralentithecium suae resembles P. aquaticum in having hyaline, 1-septate, broadly fusiform ascospores with gelatinous sheaths [35]. However, P. suae is distinct from P. aquaticum in having globose to subglobose pseudoparaphyses that are swollen at the apex and sometimes swollen at the septum. ...
During the investigation of lignicolous freshwater fungi in plateau lakes in Yunnan Province, China, eight Lentitheciaceae species were collected from five lakes viz. Luguhu, Qiluhu, Xingyunhu, Cibihu, and Xihu lake. Based on morphological characters and phylogenetic analysis of combined ITS, LSU, SSU, and tef 1-α sequence data, a new genus Paralentithecium, two new species (Paralentithecium suae, and Setoseptoria suae), three new records (Halobyssothecium phragmitis, H. unicellulare, and Lentithecium yunnanensis) and three known species viz. Halobyssothecium aquifusiforme, Lentithecium pseudoclioninum, and Setoseptoria bambusae are reported.
... Work in the 1990s included description of newer taxa from mangrove substrata and the salt marsh sedge Juncus roemerianus (Kohlmeyer and Volkmann-Kohlmeyer, 2001a-d;Kohlmeyer et al., 2005). More recently, mycologists have focused studies on the phylogeny of marine fungi and introduced many new orders and families (Kohlmeyer et al., 2000, Campbell et al., 2009, Thongkantha et al., 2009, Suetrong et al., 2009, 2011, Zhang et al., 2009a. ...
The Indian Marine Fungal Database (IMFD) is the first on-line database of information on Marine fungi from India. The database contains 233 records from 52 families, 146 genera and 233 species, and can be accessed at www.fungifromindia.com. Every species from this industrially important group of fungi has been given a unique identity number that can be cited in publications where a new species is described. Every entry in this database has been linked with the globally recognized myco-database (www.mycobank.org). The IMFD is part of an Indian initiative to promote international biodiversity documentation and form a global network of databases on biological information.
... Lentitheciaceae, Massarinaceae, Montagnulaceae (current name: Didymosphaeriaceae), Morosphaeriaceae and Trematosphaeriaceae. Currently, the family comprises 17 genera: Crassoascoma (Liu et al. 2021), Darksidea (Knapp et al. 2015), Halobyssothecium (Dayarathne et al. 2018), Katumotoa (Tanaka and Harada 2005), Keissleriella (Höhnel 1919), Lentithecium (Zhang et al. 2009b), Murilentithecium (Wanasinghe et al. 2014), Neoophiosphaerella (Tanaka et al. 2015), Phragmocamarosporium (Wijayawardene et al. 2018), Pleurophoma (Höhnel et al. 1914), Poaceascoma (Phookamsak et al. 2015, Pseudomurilentithecium , Pseudokeissleriella (Yang et al. 2022), Setoseptoria (Quaedvlieg et al. 2013), Tingoldiago (Hirayama et al. 2010), Towyspora (Li et al. 2016) and Wettsteinina (Hillebrand 1907). Some key asexual genera of Lentitheciaceae include Setoseptoria, Phragmocamarosporium, Pleurophoma and Towyspora that are all coelomycetous in nature. ...
North-western India is well-known for its unique fungal biodiversity, especially for asexual Ascomycota. The present study introduces yet another new fungus from this region, characterized by forming unusual synnemata-like structures that give rise to pigmented dictyoconidia. Phylogenetic analyses, based on SSU, LSU, ITS, and TEF1α sequence data revealed that this unique synnematous fungus formed a distinct lineage within Lentitheciaceae. Groenewaldia gen. nov., based on Groenewaldia indica, is introduced here for this fungus isolated from the bark of Eucalyptus tereticornis collected in the Hoshiyarpur area of the state of Punjab. Groenewaldia is the first synnematous member of Lentitheciaceae, having dictyosporous conidia. Phylogenetically, Groenewaldia is distant from all known genera of Lentitheciaceae and forms a basal lineage adjacent to Pseudomurilentithecium.
... The isolated fungi were studied and identified using sources and manuals like Mycology Online (Mycology Online, 2017), A Manual of Aspergilli (Thom & Raper, 1945), Key to the fungi of the genus Aschochyta Lib (Mel'nik, 2000), Aschochyta leaf spots of cereals and grasses in the United States (Sprague & Johnson, 1950), Epitypification and phylogeny of Colletotrichum acutatum (Than et al., 2008), Curvularia lunata and Phyllachora sp.: two fungal pathogens of the grassy weed Hymenachne amplexicaulis from Brazil, (Monteiro et al., 2003), Fusarium species as pathogen on orchids (Srivastava, 1971), Towards a phylogenetic clarification of Lophiostoma/Massarina and morphologically similar genera in the Pleosporales (Zhang et al., 2008), Faces of Fungi Database: fungal names linked with morphology, phylogeny and human impacts (Rai, 2020) and Sizing up Septoria (Quaedvlieg et al., 2013). ...
... Asci 110-145 × 10-15 μm, 8-spored, bitunicate, clavate, pedicellate. Ascospores 27-38 × 5-7.5 μm, biseriate, fusiform, hyaline, one septate, with 3-5 septa and faintly brown, 2-4 distinct guttule in each cell, short terminal appendages and warts when spores are senescent (Zhang et al., 2008). ...
... Lophiostoma is mentioned mostly as saprophytes under the family Lophiostomaceae (Hashimoto et al., 2018;Zhang et al., 2008). The species under this genus are recently been discovered as endophytes in the leaves of Pinus densiflora (Park & Eom, 2020). ...
Orchidaceae, one of the largest family of angiosperm, represented by their distinct morphology and flowers of natural beauty. With the increase in orchid demand globally for their ornamental purposes, orchid diseases are carried together along with their transportation. These pathogens that infect orchids, decreases their quality and market value. Thus, studies focusing on the health and diseases on orchids are found to be necessary. This study aimed to identify the phylloplane mycoflora causing fungal diseases on orchids, through the study of symptoms on hosts and laboratory culture. To identify the fungal pathogens, normal teasing and section cutting was followed. Furthermore, culturing of Phylloplane mycoflora using Czapex Dox Agar Medium (CzA) was done. The study identified disease causing pathogens as Aspergillus niger (black mould) and Ascochyta sp. (Ascochyta blight) on Eria coronaria, Colletotrichum acutatum (Black spot or Anthracnose disease) and Curvularia lunata (leaf spot) on Bulbophylum reptans, Fusarium oxysporum (Fusarium leaf spot) on Cymbidium erythraeum, Lophiostoma macrostomum (Lophiostoma leaf blight) on Cymbidium hookerianum, Phyllosticta dendrobii (Phyllosticta leaf spot) on Dendrobium nobile, and Septoria sp. (Spetoria leaf spot) on Calanthe plantaginea and Dendrobium hookerianum.
... Halobyssothecium therefore possesses immersed, semi-immersed, erumpent or superficial, carbonaceous or coriaceous, papillate, ostiolate ascomata, short-pedicellate asci with or without an ocular chamber and asymmetrically or symmetrically fusiform, straight or slightly curved, yellowish-brown or versicolored, 1-or 3-septate, verrucose or smooth-walled ascospores, asexual morphs coelomycetous or hyphomycetous. The remaining species in Lentithecium are sexual stages without known asexual morphs and are distinguished from Halobyssothecium species by hyaline ascospores with a mucilaginous sheath (Zhang et al. 2009a;Tanaka et al. 2015). In our phylogenetic tree, Halobyssothecium formed a sister clade to Lentithecium except for two uncertain species H. versicolor and L. aquaticum (Fig. 15). ...
... We recognize our collection as a distinct species based on the morphology, but further molecular data are required to confirm their relationship. (Zhang et al. 2009a;Tanaka et al. 2015;Hyde et al. 2016b;Su et al. 2016b;Crous et al. 2018b;Dong et al. 2020b). Calabon et al. (2021) redefined Lentithecium and accepted four ascomycetes, L. clioninum, L. fluviatile, L. pseudoclioninum and L. aquaticum in the genus. ...
Freshwater fungi comprises a highly diverse group of organisms occurring in freshwater habitats throughout the world. During a survey of freshwater fungi on submerged wood in streams and lakes, a wide range of sexual and asexual species were collected mainly from karst regions in China and Thailand. Phylogenetic inferences using partial gene regions of LSU, ITS, SSU, TEF1α, and RPB2 sequences revealed that most of these fungi belonged to Dothideomycetes and Sordariomycetes and a few were related to Eurotiomycetes. Based on the morphology and multi-gene phylogeny, we introduce four new genera, viz. Aquabispora, Neocirrenalia, Ocellisimilis and Uvarisporella, and 47 new species, viz. Acrodictys chishuiensis, A. effusa, A. pyriformis, Actinocladium aquaticum, Annulatascus tratensis, Aquabispora setosa, Aqualignicola setosa, Aquimassariosphaeria vermiformis, Ceratosphaeria flava, Chaetosphaeria polygonalis, Conlarium muriforme, Digitodesmium chishuiense, Ellisembia aquirostrata, Fuscosporella atrobrunnea, Halobyssothecium aquifusiforme, H. caohaiense, Hongkongmyces aquisetosus, Kirschsteiniothelia dushanensis, Monilochaetes alsophilae, Mycoenterolobium macrosporum, Myrmecridium splendidum, Neohelicascus griseoflavus, Neohelicomyces denticulatus, Neohelicosporium fluviatile, Neokalmusia aquibrunnea, Neomassariosphaeria aquimucosa, Neomyrmecridium naviculare, Neospadicoides biseptata, Ocellisimilis clavata, Ophioceras thailandense, Paragaeumannomyces aquaticus, Phialoturbella aquilunata, Pleurohelicosporium hyalinum, Pseudodactylaria denticulata, P. longidenticulata, P. uniseptata, Pseudohalonectria aurantiaca, Rhamphoriopsis aquimicrospora, Setoseptoria bambusae, Shrungabeeja fluviatilis, Sporidesmium tratense, S. versicolor, Sporoschisma atroviride, Stanjehughesia aquatica, Thysanorea amniculi, Uvarisporella aquatica and Xylolentia aseptata, with an illustrated account, discussion of their taxonomic placement and comparison with morphological similar taxa. Seven new combinations are introduced, viz. Aquabispora grandispora (≡ Boerlagiomyces grandisporus), A. websteri (≡ Boerlagiomyces websteri), Ceratosphaeria suthepensis (≡ Pseudohalonectria suthepensis), Gamsomyces aquaticus (≡ Pseudobactrodesmium aquaticum), G. malabaricus (≡ Gangliostilbe malabarica), Neocirrenalia nigrospora (≡ Cirrenalia nigrospora), and Rhamphoriopsis glauca (≡ Chloridium glaucum). Ten new geographical records are reported in China and Thailand and nine species are first reported from freshwater habitats. Reference specimens are provided for Diplocladiella scalaroides and Neocirrenalia nigrospora (≡ Cirrenalia nigrospora). Systematic placement of the previously introduced genera Actinocladium, Aqualignicola, and Diplocladiella is first elucidated based on the reference specimens and new collections. Species recollected from China and Thailand are also described and illustrated. The overall trees of freshwater Dothideomycetes and Sordariomycetes collected in this study are provided respectively and genera or family/order trees are constructed for selected taxa.
... The sexual morph of Lophiostomataceae is characterized by immersed to erumpent, carbonaceous to coriaceous ascomata, with rounded or slit-like ostiolar opening on a laterally compressed papilla, mostly clavate asci, one-to multi-septate and hyaline to dark brown ascospores with terminal appendages or mucilaginous sheaths (Hyde et al. 2013;Tennakoon et al. 2018;Thambugala et al. 2015;Zhang et al. 2009). Based on the morpho-molecular approach using 18S small subunit of the nrDNA (SSU), Internal transcribed spacer region of the nrDNA (ITS), 28S large subunit of the nrDNA (LSU), and Translation elongation factor 1-α (tef1-α) sequence data, Thambugala et al. (2015) ML analysis was performed using IQ-TREE multicore version 1.6.12 ...
Lophiostomataceae species commonly occur as saprobes on twigs, stems or bark on diverse woody and herbaceous plants in aquatic and terrestrial habitats. In the present study, we introduce Lophiostoma khanzada-kirgizbaeva sp. nov. and Paucispora xishanensis sp. nov. as new species isolated from woody substrates from Uzbekistan and China, respectively. Species delimitation was conducted using morphological and molecular phylogenetic approaches. Maximum likelihood and Bayesian inference analyses of a combined SSU, ITS, LSU, tef1-α and rpb2 sequence matrix revealed that L. khanzada-kirgizbaev and P. xishanensis are distinct from other Lophiostoma and Paucispora species in Lophiostomataceae, respectively.
... Boise (1983) considered Byssothecium to be closely related to Teichospora. Zhang et al. (2009) accepted Byssothecium as a genus in Massarinaceae. Lumbsch & Huhndorf (2010) and Wijayawardene et al. (2014) treated Byssothecium in Dothideomycetes genera incertae sedis. ...
This paper provides an updated classification of the Kingdom Fungi (including fossil fungi)
and fungus-like taxa. Five-hundred and twenty-three (535) notes are provided for newly introduced
taxa and for changes that have been made since the previous outline. In the discussion, the latest
taxonomic changes in Basidiomycota are provided and the classification of Mycosphaerellales are
broadly discussed. Genera listed in Mycosphaerellaceae have been confirmed by DNA sequence
analyses, while doubtful genera (DNA sequences being unavailable but traditionally
accommodated in Mycosphaerellaceae) are listed in the discussion. Problematic genera in
Glomeromycota are also discussed based on phylogenetic results.
... K.D. Hyde, J. Fourn. & Ying Zhang (2009: 234) as the type species (Zhang et al. 2009b). This family accommodates 14 genera and more than 80 species 104 • Phytotaxa 554 (2) © 2022 Magnolia Press (Wijayawardene et al. 2020, Calabon et al. 2021. ...
... & K.D. Hyde (2009: 234) and three new combinations such as L. arundinaceum (Sowerby) K.D. Hyde, J. Fourn. & Ying Zhang (2009: 236), L. lineare and L. fluviatile; while L. fluviatile was designated as the type species (Zhang et al. 2009b). Lentithecium species have been reported as saprobic on submerged woody plants, herbaceous in freshwater, or on culms of dead grass in terrestrial habitats (Zhang et al. 2009b, Dong et al. 2020, Calabon et al. 2021) Sexual morph is characterized by Ascomata immersed to erumpent, scattered to gregarious, uniloculate, globose to subglobose, glabrous, dark brown to black, with ostiolate papilla; Peridium consisting of several layers of thin-walled cells; Pseudoparaphyses numerous, hyaline, cellular, branched, septate, recurrent; Asci 8-spored, bitunicate, fissitunicate, clavate to oblong, widely rounded at the apex, short pedicel; Ascospores bi-or tri-seriate, fusiform, slightly curved to straight, hyaline, septate, usually asymmetrical, smooth to rough, with or without a sheath. ...
... & Ying Zhang (2009: 236), L. lineare and L. fluviatile; while L. fluviatile was designated as the type species (Zhang et al. 2009b). Lentithecium species have been reported as saprobic on submerged woody plants, herbaceous in freshwater, or on culms of dead grass in terrestrial habitats (Zhang et al. 2009b, Dong et al. 2020, Calabon et al. 2021) Sexual morph is characterized by Ascomata immersed to erumpent, scattered to gregarious, uniloculate, globose to subglobose, glabrous, dark brown to black, with ostiolate papilla; Peridium consisting of several layers of thin-walled cells; Pseudoparaphyses numerous, hyaline, cellular, branched, septate, recurrent; Asci 8-spored, bitunicate, fissitunicate, clavate to oblong, widely rounded at the apex, short pedicel; Ascospores bi-or tri-seriate, fusiform, slightly curved to straight, hyaline, septate, usually asymmetrical, smooth to rough, with or without a sheath. Several species of Lentithecium were previously considered asexual morphs (Ryckegem 2001, Zhang et al. 2009a, but with the transfer of various species, no asexual types were discovered in the remaining species. ...
We describe a new fungal species of the genus Lentithecium, collected from dead culms of Artemisia sp. in Songhua Reservoir at Kunming, Yunnan Province, China. Multi-gene phylogenetic analyses of LSU, ITS, SSU, and tef1-α sequence data showed that our new fungal strain belongs to Lentithecium and is well separated from all other extant species of Lentithecium. Lentithecium yunnanensis sp. nov. is compared with all the extant species by morphological characteristics, culture characteristics, host, and location information and is the first report of Lentithecium from China on Artemisia. A comprehensive description, a color photo plate of microcharacteristics, and a phylogenetic tree to show the position of the new species are provided.
... DNA was extracted from fungal mycelia by using a modified protocol published by Crous et al. (2009), and PCR was conducted using ITS1-F/ITS4-R (White et al., 1990) and EF-688F/EF1-1251R (Alves et al., 2008) primer pairs. PCR amplification and sequencing was conducted as described by Zhang et al. (2009). A BLAST search in GenBank using the ITS and EF-1a sequences revealed 100% identity with both sequences of P. circumscissa (KT428056, KX853061). ...
Cherry leaf spot (CLS), caused by Passalora circumscissa , is a fungal disease that can cause decreased fruit quality and yield via inconsistent ripening or premature defoliation. Germplasm resource screening is the most reliable approach to disease control for economically important crops. However, information is limited in China about the resistance of cherry cultivars to leaf spot caused by P. circumscissa . The aim of this study was to identify the resistance levels of cherry cultivars. Fifty-two cherry cultivars, including 40 Prunus avium , four Prunus pseudocerasus , and eight Prunus cerasus cultivars were collected for resistance level characterization. These specimens were then used to screen for P. circumscissa resistance through both detached leaf assays and natural field infection. Significant differences in the disease index (DI) value among test cultivars, ranging from 5.7 (resistant) to 53.7 (highly susceptible) and 6.5 (resistant) to 53.2 (highly susceptible), were observed under the controlled and field conditions respectively. Correlation coefficients between DI in pairs of years were highly significant (0.77–0.86). Although resistance rankings for cherry cultivars between screening methods were variable, the resistance levels of 52 cultivars evaluated under controlled and field condition were comparable with a correlation coefficient of 0.70 ( P < 0.01). The results indicated that, across cherry cultivars, responses to CLS in the detached leaf assay corresponded well to responses under field conditions. A detached leaf assay was developed as a complementary method to facilitate the screening of cherry cultivars for resistance to leaf spot caused by P. circumscissa . Our study provides a theoretical basis for cherry disease resistance breeding and rational cultivar utilization.
