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(A) Grayish, glistening mucoid colonies of M. timonae on blood agar. (B) Gram stain revealed abundant gram-negative straight rods.
Source publication
Massilia timonae infections in humans have rarely been reported. To the best of our knowledge, M. timonae has not been previously recognized as a causative agent of obstetric or gynecological infections. Timely identification of this unusual pathogen and the use of targeted antimicrobial therapy are crucial to avoid consequences and treatment failu...
Context in source publication
Context 1
... / L (4-11.0), CRP 251 (0-5 mg/L), Procalcitonin 6 µg/L (< 0.05), and 2.4 × 1.3 cm remnant or blood clots were seen ultrasonography within the uterine cavity (Fig. 1). Therefore, piperacillin/tazobactam was started empirically and 2 days later, non-fermentative gram-negative bacilli grew from four bottles of blood culture and a high vaginal swab ( Fig. 2A and 2B). Subsequently, MALDI (TOF-MS, Billerica, MA, USA) identified the organism as Massilia timonae. The fever resolved quickly, and the antimicrobial was switched to ciprofloxacin according to sensitivity (Table 1). Follow-up US of the pelvis showed no evidence of previously reported remnants; thus, ciprofloxacin was continued for 14 days ...
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Antimicrobial efficacy is traditionally described by a single value, the minimal inhibitory concentration (MIC), which is the lowest concentration that prevents visible growth of the bacterial population. As a consequence, bacteria are classically qualitatively categorized as resistant if therapeutic concentrations are below MIC and susceptible oth...
Citations
... This notion is supported by the significant enrichment of infection-associated bacteria from human clinical samples (classified as human others) in the AD brains ( Fig 4C). Notably, most of these bacteria have connections to infectious diseases such as sepsis, genital ulcer disease, peritonitis, brain abscess, and septic abortion [31][32][33][34][35][36][37][38]. In fact, any common systemic infections (sepsis, pneumonia, other lower respiratory tract infections, urinary tract infections, and skin and soft tissue infections) are known to elevate the risk of dementia diagnosis, as evidenced in a population-based cohort study [39]. ...
Alzheimer’s disease (AD) is a neurodegenerative disease accompanied by neuroimmune inflammation in the frontal cortex and hippocampus. Recently, the presence of bacteria in AD-affected brains has been documented, prompting speculation about their potential role in AD-associated neuroinflammation. However, the characterization of bacteriota in human brains affected by AD remains inconclusive. This study aimed to investigate potential associations between specific bacteria and AD pathology by examining brain tissues from AD-associated neurodegenerative regions (frontal cortex and hippocampus) and the non-AD-associated hypothalamus. Employing 16S rRNA gene sequencing, 30 postmortem brain tissue samples from four individuals with normal brain histology (N) and four AD patients were analyzed, along with three blank controls. A remarkably low biomass characterized the brain bacteriota, with their overall structures delineated primarily by brain regions rather than the presence of AD. While most analyzed parameters exhibited no significant distinction in the brain bacteriota between the N and AD groups, the unique detection of Cloacibacterium normanense in the AD-associated neurodegenerative regions stood out. Additionally, infection-associated bacteria, as opposed to periodontal pathogens, were notably enriched in AD brains. This study’s findings provide valuable insights into potential link between bacterial infection and neuroinflammation in AD.
... The genus Massilia consists of gram-negative bacteria of the family Oxalobacteraceae [44]. The members of this genus were primarily isolated from environmental sources [45,46]; however, in rare cases, some species are also known to cause infections in immunocompromised patients [47][48][49]. The abundance of bacteria of this genus was found to be increased in the intestinal microbiome of patients with viral gastroenteritis [50]. ...
Background and Aim
Mastitis is recognized as the most common disease in cattle and causes economic losses in the dairy industry. A number of opportunistic bacterial taxa have been identified as causative agents for this disease. Conventionally, antibiotics are used to treat mastitis; however, most bacteria are resistant to the majority of antibiotics. This study aimed to use molecular methods to identify milk microbiome patterns characteristic of mastitis that can help in the early diagnosis of this disease and in the development of new treatment strategies.
Materials and Methods
To evaluate the microbiome composition, we performed NGS sequencing of the 16S rRNA gene of the V3 region.
Results
An increase in the abundance of the bacterial genera Hymenobacter and Lachnospiraceae NK4A136 group is associated with the development of subclinical and clinical mastitis in dairy cows. These bacteria can be added to the list of markers used to detect mastitis in cows. Furthermore, a decrease in the abundance of Ralstonia, Lachnospiraceae NK3A20 group, Acetitomaculum, Massilia, and Atopostipes in cows with mastitis may indicate their role in maintaining a healthy milk microbiome. Antibiotics reduced the levels of Streptococcus in milk compared to those in the healthy group and cows before antibiotic treatment. Antibiotic therapy also contributed to an increase in the abundance of beneficial bacteria of the genus Asticcacaulis.
Conclusion
This study expands our understanding of the association between milk microbiota and mastitis.
... Apart from the above-described ones, there is also a less abundant representation of some environmentalassociated taxa, many of which have been isolated from varied environments such as Deinococcus, Domibacillus, Pantoea or Sphingomonas 57-60 . Others have been linked to isolated cases of fungaemia, bacteremia or sepsis, such as Aureobasidium, Kosakonia, Lysinibacillus or Massilia [61][62][63][64] . However, Massilia is also a common soil-inhabitant 65,66 . ...
Microorganisms colonize all possible ecological habitats, including those subjected to harsh stressors such as UV radiation. Hospitals, in particular the UV cabins used in phototherapy units, constitute an environment in which microbes are intermittently subjected to UV irradiation. This selective pressure, in addition to the frequent use of antibiotics by patients, may represent a threat in the context of the increasing problem of antimicrobial resistance. In this work, a collection of microorganisms has been established in order to study the microbiota associated to the inner and outer surfaces of UV cabins and to assess their resistance to UV light and the antibiotics frequently used in the Dermatology Service of a Spanish hospital. Our results show that UV cabins harbor a relatively diverse biocenosis dominated by typically UV-resistant microorganisms commonly found in sun-irradiated environments, such as Kocuria, Micrococcus or Deinococcus spp., but also clinically relevant taxa, such as Staphylococcus or Pseudomonas spp. The UV-radiation assays revealed that, although some isolates displayed some resistance, UV is not a major factor shaping the biocenosis living on the cabins, since a similar pool of resistant microorganisms was identified on the external surface of the cabins. Interestingly, some Staphylococcus spp. displayed resistance to one or more antibiotics, although the hospital reported no cases of antibiotic-resistance infections of the patients using the cabins. Finally, no association between UV and antibiotic resistances was found.
... In the other hand, urban soils presented, along with hen house soils, the highest diversity indexes (Table S3), which could be explained by the accumulation of human activity wastes and by-products in the former, as well as the introduction of gastrointestinal microbiota members in the latter [36]. Both soils were abundant with Massilia (phylum Pseudomonadota), common environmental bacteria that have been shown to cause opportunistic infections in immunocompromised patients [37,38]. ...
Land-use conversion changes soil properties and their microbial communities, which, combined with the overuse of antibiotics in human and animal health, promotes the expansion of the soil resistome. In this context, we aimed to profile the resistome and the microbiota of soils under different land practices. We collected eight soil samples from different locations in the countryside of São Paulo (Brazil), assessed the community profiles based on 16S rRNA sequencing, and analyzed the soil metagenomes based on shotgun sequencing. We found differences in the communities’ structures and their dynamics that were correlated with land practices, such as the dominance of Staphylococcus and Bacillus genera in agriculture fields. Additionally, we surveyed the abundance and diversity of antibiotic resistance genes (ARGs) and virulence factors (VFs) across studied soils, observing a higher presence and homogeneity of the vanRO gene in livestock soils. Moreover, three β-lactamases were identified in orchard and urban square soils. Together, our findings reinforce the importance and urgency of AMR surveillance in the environment, especially in soils undergoing deep land-use transformations, providing an initial exploration under the One Health approach of environmental levels of resistance and profiling soil communities.
Microorganisms colonize all possible ecological habitats, including those subjected to harsh stressors such as UV radiation. Hospitals, in particular the UV cabins used in phototherapy units, constitute an environment in which microbes are intermittently subjected to UV irradiation. This selective pressure, in addition to the frequent use of antibiotics by patients, may represent a threat in the context of the increasing problem of antimicrobial resistance. In this work, a collection of microorganisms has been established in order to study the microbiota associated to the inner and outer surfaces of UV cabins and to assess their resistance to UV light and the antibiotics frequently used in the Dermatology Service of a Spanish hospital. Our results show that UV cabins harbor a relatively diverse biocenosis dominated by typically UV-resistant microorganisms commonly found in sun-irradiated environments, such as Kocuria , Micrococcus or Deinococcus spp., but also clinically relevant taxa, such as Staphylococcus or Pseudomonas spp. The UV-radiation assays revealed that, although some isolates displayed some resistance, UV is not a major factor shaping the biocenosis living on the cabins, since a similar pool of resistant microorganisms was identified on the external surface of the cabins. Interestingly, some Staphylococcus spp. displayed resistance to one or more antibiotics, although the hospital reported no cases of antibiotic-resistance infections of the patients using the cabins. Finally, no association between UV and antibiotic resistances was found.
Anthropization in terrestrial environments commonly leads to land use transformation, changing soil properties and their microbial communities. This, combined with the exacerbated use of antibiotics in human and animal health promotes the expansion of the soil resistome. Considering the urgent need for surveillance of antimicrobial resistance (AMR), we aimed to evaluate how different land practices (urban, farming and forest) can affect the soil resistome and the dynamics of their bacterial communities. We collected eight soil samples from different locations in the countryside of Sao Paulo (Brazil), assessed the community profiles based on 16S rRNA sequencing and analyzed the soil metagenomes based on shotgun sequencing. Our results highlight differences in the communities' structure and their dynamics which were correlated with land practices. Additionally, differences were observed in the abundance and diversity of antibiotic resistance genes (ARGs) and virulence factors (VFs) across studied soils, where a higher presence and homogeneity of vanRO, mtrA and rbpA genes were detected in livestock soils. We observed that Staphylococcus and Bacillus are positively correlated with each other and are markers for agricultural communities. Moreover, the abundance and diversity of ARGs and VFs observed in farming soils raises concerns regarding the potential spread of these genes in the environment. Together, our findings reinforce the importance and urgency of AMR surveillance in the environment, especially in soils undergoing deep land use transformations due to anthropic activity.
