Fig 5 - uploaded by Ansar Javeed
Content may be subject to copyright.
(A/B) The blood clotting index (BCI) of different hydrogel samples in whole blood coagulation at 5 and 10 min, respectively. (C) Photographs of the blood clots remaining on the sample surface and their supernatant after 10 min of incubation. Analysis of (D) activated partial thromboplastin time (APTT), (E) prothrombin time (PT), (F) thrombin time (TT), and (G) fibrinogen (FIB) of platelet-poor plasma (PPP) upon exposure to different hydrogel samples (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001, NS: no significant difference.
Contexts in source publication
Context 1
... bonds, coordination bonds, hydrophobic interactions, and covalent bonds) between the catechol groups of TA and the nucleophilic groups (e.g., thiol or amino groups) on the tissue lay the ground for excellent bio-adhesion of hydrogels [50,51]. Moreover, the TA@SMF-0.5 adhered tightly onto not only major organs of mice and various material surfaces (Fig. S5), but also human skin and pig skin under sustained dynamic water flow for>60 s (Movie. S1), illustrating its potential as an ideal hemostatic adhesive dressing for widespread clinical ...
Context 2
... assess the whole blood coagulability of different materials in vitro, and a lower value means a better pro-coagulant effect [59,60]. Compared with the blank control and PB gel, the addition of SMF, TA, or TA@SMF all promoted the hemostatic performance of the hydrogel, and among them SMF/PB appeared with the lowest BCI of 49.64 ± 1.86% (P < 0.001, Fig. 5A). SMF initiates coagulation faster than TA, which may be related to the fact that SMFs increase the surface roughness of pore wall and is more conducive to fibrinogen adsorption and hemocyte adhesion [19]. After interacting with blood for 10 min, the SMF/PB and TA@SMF-0.5 showed the lowest BCI for only 38.22 ± 2.21% and 40.77 ± 2.27% ...
Context 3
... coagulation faster than TA, which may be related to the fact that SMFs increase the surface roughness of pore wall and is more conducive to fibrinogen adsorption and hemocyte adhesion [19]. After interacting with blood for 10 min, the SMF/PB and TA@SMF-0.5 showed the lowest BCI for only 38.22 ± 2.21% and 40.77 ± 2.27% (P > 0.05), respectively (Fig. 5B). Further, Fig. 5C shows photographs of the blood clots remaining on the sample surface and their supernatant after 10 min of incubation. It is obvious that the solutions of SMF/PB and TA@SMF-0.5 exhibited a light reddish color, indicating that most blood cells coagulated on these two hydrogels. Fig. S9A shows the blood fluidity ...
Context 4
... than TA, which may be related to the fact that SMFs increase the surface roughness of pore wall and is more conducive to fibrinogen adsorption and hemocyte adhesion [19]. After interacting with blood for 10 min, the SMF/PB and TA@SMF-0.5 showed the lowest BCI for only 38.22 ± 2.21% and 40.77 ± 2.27% (P > 0.05), respectively (Fig. 5B). Further, Fig. 5C shows photographs of the blood clots remaining on the sample surface and their supernatant after 10 min of incubation. It is obvious that the solutions of SMF/PB and TA@SMF-0.5 exhibited a light reddish color, indicating that most blood cells coagulated on these two hydrogels. Fig. S9A shows the blood fluidity variations after the ...
Context 5
... factors II, VII, and X. TT and FIB are used to assess the conversion time of fibrinogen to fibrin and the content of fibrinogen, respectively [3,32]. Compared with the blank control, the APTT, PT, TT, and FIB values of PPP changed slightly after incubation for 30 min with different hydrogels, while they were all within the normal range (Fig. 5D-G). These results indicated that the studied samples do not adsorb coagulation factors in PPP or initiate coagulation cascade events during incubation, leading to a decrease in coagulation factor concentration, nor do they release substances into PPP to affect the activity of coagulation ...
Citations
... Most hydrogel [8][9][10][11] Three-dimensional, cross-linked networks of hydrophilic polymer chains Adhesion Catechol groups [ 33,121,122] -stacking, cation-interaction, hydrogen bonding, and covalent cross-linking Dry cross-linking mechanism [ 123] Hydrogen bonds, electrostatic interactions, and covalent bonds Activated ester, isocyanate, and aldehyde functional groups [ 124] Covalent bonds Supramolecular polymeric [ 125] Hydrogen bonds Self-healing Phenylboronic acid groups and the hydroxyl groups [ 126] Dynamic boronic acid ester bonds Dynamic Schiff base network [ 127] Schiff base bonds and ions coordination bonds o-phthalaldehyde/amine (hydrazide) [ 128] Hydrazone linkages PVA [ 126,129] Hydrogen bonds ...
Hydrogels have emerged as promising candidates for biomedical applications, especially in the field of antibacterial therapeutics, due to their unique structural properties, highly tunable physicochemical properties, and excellent biocompatibility. The integration of stimuli‐responsive functions into antibacterial hydrogels holds the potential to enhance their antibacterial properties and therapeutic efficacy, dynamically responding to different external or internal stimuli, such as pH, temperature, enzymes, and light. Therefore, this review describes the applications of hydrogel dressings responsive to different stimuli in antibacterial therapy. The collaborative interaction between stimuli‐responsive hydrogels and antibacterial materials was discussed. This synergistic approach, in contrast to conventional antibacterial materials, not only amplifies the antibacterial effect but also alleviates adverse side effects and diminishes the incidence of multiple infections and drug resistance. The review provides a comprehensive overview of the current challenges and outlines future research directions for stimuli‐responsive antibacterial hydrogels. It underscores the imperative for ongoing interdisciplinary research aimed at unraveling the mechanisms of wound healing. This understanding is crucial for optimizing the design and implementation of stimuli‐responsive antibacterial hydrogels. Ultimately, this review aims to offer scientific guidance for the development and practical clinical application of stimuli‐responsive antibacterial hydrogel dressings.
This article is protected by copyright. All rights reserved
... The hemostatic hydrogel www.advancedsciencenews.com www.small-journal.com developed by Si et al. [145] facilitates the swift absorption of substantial plasma volumes. With its high liquid absorption capacity and robust adhesion, the hydrogel establishes a mechanical barrier, ensuring secure attachment to the wound surface even amid bleeding. ...
Hemorrhage remains a critical challenge in various medical settings, necessitating the development of advanced hemostatic materials. Hemostatic hydrogels have emerged as promising solutions to address uncontrolled bleeding due to their unique properties, including biocompatibility, tunable physical characteristics, and exceptional hemostatic capabilities. In this review, a comprehensive overview of the preparation and biomedical applications of hemostatic hydrogels is provided. Particularly, hemostatic hydrogels with various materials and forms are introduced. Additionally, the applications of hemostatic hydrogels in trauma management, surgical procedures, wound care, etc. are summarized. Finally, the limitations and future prospects of hemostatic hydrogels are discussed and evaluated. This review aims to highlight the biomedical applications of hydrogels in hemorrhage management and offer insights into the development of clinically relevant hemostatic materials.
... The initial stage was the sterilization of tannic acid. As a microbicidal substance, tannic acid could deliver the sterilization effect by disrupting the bacterial structure and disrupting the physiological process of microorganisms [49]. The second protective layer was owed to the weak electrical field generated by the surface fabric. ...
The quest for efficient hemostatic agents in emergency medicine is critical, particularly for managing massive hemorrhages in dynamic and high‐pressure wound environments. Traditional self‐gelling powders, while beneficial due to their ease of application and rapid action, fall short in such challenging conditions. To bridge this gap, the research introduces a novel self‐gelling powder that combines ultrafast covalent gelation and robust wet adhesion, presenting a significant advancement in acute hemorrhage control. This ternary system comprises ε ‐polylysine ( ε ‐PLL) and 4‐arm polyethylene glycol succinyl succinate (4‐arm‐PEG‐NHS) forming the hydrogel framework. Na 2 HPO 4 functions as the “H ⁺ sucker” to expedite the amidation reaction, slashing gelation time to under 10 s, crucial for immediate blood loss restriction. Moreover, PEG chains' hydrophilicity facilitates efficient absorption of interfacial blood, increasing the generated hydrogel's cross‐linking density and strengthens its tissue bonding, thereby resulting in excellent mechanical and wet adhesion properties. In vitro experiments reveal the optimized formulation's exceptional tissue compliance, procoagulant activity, biocompatibility and antibacterial efficacy. In porcine models of heart injuries and arterial punctures, it outperforms commercial hemostatic agent Celox, confirming its rapid and effective hemostasis. Conclusively, this study presents a transformative approach to hemostasis, offering a reliable and potent solution for the emergency management of massive hemorrhage.
Conventional hemostatic agents face challenges in achieving rapid hemostasis and effective tissue repair due to limited hemostatic scenarios, suboptimal efficacy, and inadequate adhesion to wet tissues. Drawing inspiration from nature‐sourced materials, we designed a gelatin‐based adhesive hydrogel (AOT), easily prepared and quick to form, driven by Schiff base and multiple hydrogen bonds for applications in arterial and liver bleeding models. AOT exhibited exceptional adhesion to wet tissues (48.67 ± 0.16 kPa) and displays superior hemostatic properties with reduced blood loss and hemostatic time compared to other hydrogels and conventional hemostatic materials. Moreover, AOT hydrogel exhibited good biocompatibility and biodegradability. In summary, this easily prepared adhesisve hydrogel has the potential to supplant traditional hemostatic agents, offering a novel approach to achieve swift sealing of hemostasis and facilitate wound healing and repair in broader application scenarios, owing to its unique advantages.
This article is protected by copyright. All rights reserved
