Fig 6 - uploaded by Brian A. Perry
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A, B. Mycena ulmi, SEM. A. Intermediate stage with distinct zone separating the pileus and stipe surface hyphae, with no suggestion of an organized layer of wrapping hyphae. Scale bar = 200 µm. B. Close-up of intermediate stage with distinct zone separating the developing pileus and stipe regions. Scale bar = 50 µm. C. Close-up of lamellae separating from stipe surface to form a distinct pseudocollarium. Scale bar = 1.0 mm.
Source publication
A Mycena species new to science was obtained from moist chamber cultures of trunk bark of a living American elm tree (Ulmus americana) located in the Fort Worth Botanic Garden, Tarrant County, Texas. This discovery was part of an ongoing study of corticolous myxomycetes on larger American elm trees occurring in Fort Worth nature parks. More than 15...
Context in source publication
Context 1
... hyphae. The pileus appears to completely envelope any developing stipe tissues, with these only being revealed when the stipe initiates elongation and effectively lifts the pileus off the substrate. A distinct zone can clearly be seen separating the pileus and stipe surface hyphae, with no suggestion of an organized layer of wrapping hyphae (Fig. 6A,B). The SEM and LM images clearly demonstrate the dense, minute pubescence of the stipe and pileus surfaces (Fig. ...
Similar publications
In this study, phylogenetic analysis of some fungal species distributed in West Kazakhstan was performed based on ITS sequences. All of the mushroom samples were collected from different regions of West Kazakhstan and brought to the laboratory. Total genomic DNA was extracted using a GeneMark commercial kit. ITS1 and ITS4 primers were used for the...
Citations
... The first general use of moist chamber cultures (MCs) to isolate myxomycetes (plasmodial slime molds) from live tree trunk bark is attributed to G. W. Martin and H. C. Gilbert, working in the Mycological Laboratory of the Department of Botany at the University of Iowa (Gilbert and Martin, 1933;Gilbert, 1934). Rare and newly described species were included in their original list of myxomycetes, and more recently, additional new and rare species of myxomycetes have been found using the MC technique (Keller andBrooks, 1976, 1977;Keller, 2004;Keller et al., 2008Keller et al., , 2009Keller and Marshall, 2019) as well as fungi (Perry et al., 2020) (Figure 1). Some of these species were discovered using a rope-climbing technique to collect bark samples for MC cultures in the tree canopy (Parker and Keller, 2003;Snell and Keller, 2003;Keller et al., 2004;Everhart et al., 2009;Kilgore et al., 2009;Scarborough et al., 2009). ...
... Many researchers may be in academic or private institutions where funding is lacking or inadequate, physical access to research laboratories may not be possible, or informed F I G U R E 1 Fleshy mushroom Mycena ulmi growing on the surface of live Ulmus americana tree bark in a moist chamber culture. (Photo by Brian A. Perry, reproduced with permission from the Journal of the Botanical Research Institute of Texas; Perry et al., 2020). ...
... These batch cultures have resulted in harvesting many tiny myxomycete species (100-200 µm), especially in the genera Echinostelium and Licea, or slightly larger sizes for Macbrideola species (Figure 3), enabling many more myxomycete fruiting bodies per collection deposited in the herbarium of the Botanical Research Institute of Texas (BRIT). This modified MC culture was developed at BRIT and has been used in published papers (Keller and Marshall, 2019;Perry et al., 2020). Proper curation and labeling become extremely important, especially when describing species that are new to science ( Figure 4). ...
Premise
Traditional moist chamber cultures (MCs) prepared in aseptic laboratory environments using sterile Petri dishes are commonly used to quantify the microbiota of rough‐bark tree species and woody vines. MCs are typically expensive and may be difficult to make, so a less expensive option made from easily available supplies was developed. These cost‐friendly MCs were compared with standard laboratory methods to demonstrate their efficacy.
Methods and Results
Modified MCs were made using inexpensive, store‐bought supplies; compared to a standard laboratory setting, the modified MCs are shown to be less expensive with a faster setup time and larger size that facilitates a variety of tree and woody vine species. MC use resulted in the discovery of new species of fungi and myxomycetes with associated locality records. We provide detailed instructions for creating modified MCs, as well as a list of myxomycete species and their associated bark characteristics, pH values, and water‐holding capacity.
Conclusions
This new, low‐cost MC technique makes the study of microbiota more inclusive and accessible for those in research laboratories, classrooms, and homes, including both amateurs and professionals. MCs are easy to prepare, versatile, and applicable for many areas of botany and the biological sciences, potentially allowing exploration into unexplored areas in urban ecosystems.
... and M. cyanorhiza Quél. in Europe, M. indigotica Wei & Kirschner and M. lazulina Har. Takah., Taneyama and Terashima & Oba in Asia (Smith 1947;Maas Geesteranus 1980, 1992a, 1992bPerry 2002;Robich 2003;Aronsen and Laessøe 2016;Terashima et al. 2016;Wei and Kirschner 2019;Perry et al. 2020). A bluish tint is often present on the pileus or stipe of these five species, four of them being classified into three sections: sect. ...
... Viscipelles Kühner, but M. indigotica has tubes confused with members of Favolaschia (Pat.) Pat. and not assigned any section (Smith 1947;Maas Geesteranus 1980, 1992a, 1992bPerry 2002;Robich 2003;Aronsen and Laessøe 2016;Terashima et al. 2016;Wei and Kirschner 2019;Perry et al. 2020). The three known bluish Mycena species from the Southern Hemisphere are M. caesiocana Singer, M. cyanosyringea Singer and M. interrupta (Berkeley) Sacc. ...
... In addition, M. cyanocephala Singer described from Chile, is considered to be synonymous with M. interrupta (Grgurinovic 2003). Although M. cyanorhiza, from the Northern Hemisphere, also has a blue stipe base similar to M. interrupta, but differs in pale brown to pale grey pileus and smaller basidiospores and cheilocystidia (Robich 2003;Aronsen and Laessøe 2016;Perry et al. 2020). ...
Bluish Mycena are rare, but constitute a taxonomically complex group. A total of eight bluish species in four sections have previously been reported from North America, Europe, Oceania and Asia. Two species with a blue pileus, collected in China during our taxonomic study of Mycena s.l., are described here as new to science: Mycena caeruleogrisea sp. nov. and M. caeruleomarginata sp. nov. Detailed descriptions, line drawings and a morphological comparison with closely-related species, especially herbarium specimens of M. subcaerulea from the USA, are provided. The results of Bayesian Inference and Maximum Likelihood phylogenetic analyses of a dataset of 96 nuclear rDNA ITS and 20 nLSU sequences of 43 Mycena species are also presented. The morphological data and the results of the phylogenetic analyses support the introduction of M. caeruleogrisea and M. caeruleomarginata as new species. A taxonomic key to bluish Mycena species of sections Amictae , Cyanocephalae , Sacchariferae and Viscipelles is provided.
... Las especies estudiadas de la sección Viscipelles, aunque agrupadas en el árbol, parecen conformar un grupo poco homogéneo (Perry et al. 2020) en el que deben realizarse más estudios filogenéticos. Pudiendo confirmar que M. marocana es coespecífica con M. pachyderma Kühner, circunstancia ya apuntada por Esteve Raventós & Villarreal (2003). ...
Mycena discobasis Métrod is registered from the Los Alcornocales Natural Park (Cádiz) for the first time in Europe. A detailed macro and microscopic descripion is provided, as well as a phylogeneic analysis and a comparative study is carried out with other taxa of the section Exornatae and other species of related sections.
