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Eggs of Xyleninae: 13-Rusina ferruginea (x600); 14-Charanyca trigrammica (x130); 15Charanyca trigrammica (x540); 16-Charanyca trigrammica (x660); 17-Cosmia trapezina (x130); 18-Cosmia trapezina (x940). Scale bars: 14, 17-100 μm; 13, 15, 16, 18-10 μm.

Eggs of Xyleninae: 13-Rusina ferruginea (x600); 14-Charanyca trigrammica (x130); 15Charanyca trigrammica (x540); 16-Charanyca trigrammica (x660); 17-Cosmia trapezina (x130); 18-Cosmia trapezina (x940). Scale bars: 14, 17-100 μm; 13, 15, 16, 18-10 μm.

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The Chorionic Sculpture of the Eggs of Some Xyleninae (Lepidoptera, Noctuidae) Eggs of sixteen species of fifteen genera of the subfamily Xyleninae occurring in Ukraine are described and illustrated with scanning electron microphotographs. The diagnostic characters of the genera and species are se-lected.

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... Alvah Peterson publicó durante la década de 1960 imágenes correspondientes a taxones de 35 familias; también reportó cambios de coloración, así como técnicas de fotografía para huevos (Peterson 1960(Peterson , 1961(Peterson , 1962a(Peterson ,b, 1963a(Peterson ,b, 1964(Peterson , 1965a(Peterson ,b, 1966(Peterson , 1967a(Peterson ,b, 1968(Peterson , 1970. Desde la década de 1970 se produjeron trabajos en Riodinidae y Lycaenidae primero -luego en Noctuidae, con descripciones y análisis morfológicos del corion con base en técnicas de microscopía electrónica de barrido (MEB) (Downey y Allyn 1980Dolinskaya y Geryak 2010;Dolinskaya 2010Dolinskaya , 2011Dolinskaya , 2014Dolinskaya y Ponomarenko 2013). Más tarde se extendieron estas exploraciones en huevos de la familia Hesperiidae (Hernández-Roldán et al. 2011 y en mayor número de especies de Lycaenidae paleárticas (Munguira et al. 2015). ...
... Alvah Peterson publicó durante la década de 1960 imágenes correspondientes a taxones de 35 familias; también reportó cambios de coloración, así como técnicas de fotografía para huevos (Peterson 1960(Peterson , 1961(Peterson , 1962a(Peterson ,b, 1963a(Peterson ,b, 1964(Peterson , 1965a(Peterson ,b, 1966(Peterson , 1967a(Peterson ,b, 1968(Peterson , 1970. Desde la década de 1970 se produjeron trabajos en Riodinidae y Lycaenidae primero -luego en Noctuidae, con descripciones y análisis morfológicos del corion con base en técnicas de microscopía electrónica de barrido (MEB) (Downey y Allyn 1980Dolinskaya y Geryak 2010;Dolinskaya 2010Dolinskaya , 2011Dolinskaya , 2014Dolinskaya y Ponomarenko 2013). Más tarde se extendieron estas exploraciones en huevos de la familia Hesperiidae (Hernández-Roldán et al. 2011 y en mayor número de especies de Lycaenidae paleárticas (Munguira et al. 2015). ...
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... Alvah Peterson publicó durante la década de 1960 imágenes correspondientes a taxones de 35 familias; también reportó cambios de coloración, así como técnicas de fotografía para huevos (Peterson 1960(Peterson , 1961(Peterson , 1962a(Peterson ,b, 1963a(Peterson ,b, 1964(Peterson , 1965a(Peterson ,b, 1966(Peterson , 1967a(Peterson ,b, 1968(Peterson , 1970. Desde la década de 1970 se produjeron trabajos en Riodinidae y Lycaenidae primero -luego en Noctuidae, con descripciones y análisis morfológicos del corion con base en técnicas de microscopía electrónica de barrido (MEB) (Downey y Allyn 1980, 1981, 1984Dolinskaya y Geryak 2010;Dolinskaya 2010Dolinskaya , 2011Dolinskaya , 2014Dolinskaya y Ponomarenko 2013). Más tarde se extendieron estas exploraciones en huevos de la familia Hesperiidae (Hernández-Roldán et al. 2011 y en mayor número de especies de Lycaenidae paleárticas (Munguira et al. 2015). ...
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... En años recientes, los estudios sobre huevos de mariposas y en particular de su capa más externa -el exocorion-se realizaron con el empleo de dos técnicas de visualización: el empleo de microscopio electrónico de barrido (MEB) y la tinción con cloruro de metiltionina (azul de metileno) con observaciones en microscopio estereoscópico (Nieves-Uribe et al. 2016a). Estos trabajos se efectuaron en Noctuidae (Dolinskaya y Geryak 2010;Dolinskaya 2010Dolinskaya , 2011Dolinskaya y Ponomarenko 2013), Hesperiidae (Hernández-Roldán et al. 2012), Pieridae (Eitschberger y Ströhle 1990;Hernández-Mejía et al. 2013, 2014aNieves-Uribe et al. 2016a,b, 2018aLlorente-Bousquets et al. 2018), Nymphalidae (Heliconiini: Dell'Erba et al. 2005Biblidinae: Nieves-Uribe et al. 2015, 2016d, 2017a,b, 2019Satyrinae: Thomson 1992, García-Barros y Martín 1995, Lycaenidae (Downey y Allyn 1981, Munguira et al. 2015, y Riodinidae (Downey y Allyn 1980). Los huevos en la mayoría de estos estudios, así como otros donde se abarcan principalmente ciclos de vida, se obtienen mediante la observación y recolección de oviposiciones de huevos fecundados en el campo o en el laboratorio (v. ...
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... En años recientes, los estudios sobre huevos de mariposas y en particular de su capa más externa -el exocorion-se realizaron con el empleo de dos técnicas de visualización: el empleo de microscopio electrónico de barrido (MEB) y la tinción con cloruro de metiltionina (azul de metileno) con observaciones en microscopio estereoscópico (Nieves-Uribe et al. 2016a). Estos trabajos se efectuaron en Noctuidae (Dolinskaya y Geryak 2010;Dolinskaya 2010Dolinskaya , 2011Dolinskaya y Ponomarenko 2013), Hesperiidae (Hernández-Roldán et al. 2012), Pieridae (Eitschberger y Ströhle 1990;Hernández-Mejía et al. 2013, 2014a,b, 2015Nieves-Uribe et al. 2016a,b, 2018aLlorente-Bousquets et al. 2018), Nymphalidae (Heliconiini: Dell'Erba et al. 2005Biblidinae: Nieves-Uribe et al. 2015, 2016d, 2017a,b, 2019; Satyrinae: Thomson 1992, García-Barros y Martín 1995), Lycaenidae (Downey y Allyn 1981, Munguira et al. 2015, y Riodinidae (Downey y Allyn 1980). Los huevos en la mayoría de estos estudios, así como otros donde se abarcan principalmente ciclos de vida, se obtienen mediante la observación y recolección de oviposiciones de huevos fecundados en el campo o en el laboratorio (v. ...
... En años recientes, los estudios sobre huevos de mariposas y en particular de su capa más externa -el exocorion-se realizaron con el empleo de dos técnicas de visualización: el empleo de microscopio electrónico de barrido (MEB) y la tinción con cloruro de metiltionina (azul de metileno) con observaciones en microscopio estereoscópico (Nieves-Uribe et al. 2016a). Estos trabajos se efectuaron en Noctuidae (Dolinskaya y Geryak 2010;Dolinskaya 2010Dolinskaya , 2011Dolinskaya y Ponomarenko 2013), Hesperiidae (Hernández-Roldán et al. 2012), Pieridae (Eitschberger y Ströhle 1990;Hernández-Mejía et al. 2013, 2014aNieves-Uribe et al. 2016a,b, 2018aLlorente-Bousquets et al. 2018), Nymphalidae (Heliconiini: Dell'Erba et al. 2005Biblidinae: Nieves-Uribe et al. 2015, 2016d, 2017a,b, 2019Satyrinae: Thomson 1992, García-Barros y Martín 1995, Lycaenidae (Downey y Allyn 1981, Munguira et al. 2015, y Riodinidae (Downey y Allyn 1980). Los huevos en la mayoría de estos estudios, así como otros donde se abarcan principalmente ciclos de vida, se obtienen mediante la observación y recolección de oviposiciones de huevos fecundados en el campo o en el laboratorio (v. ...
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We studied the chorionic morphology of six species of Hamadryas, and together with previous studies, we compared our results with previously published phylogenies for the genus. Samples were obtained from 19 females collected between 2013 and 2017 whose abdomens were sectioned and preserved for later dissection. Eggs were extracted from those dissections and used for the descriptions and illustrations of the chorion. The Hamadryas egg is of the globose type; it is quasi-spheroidal and has multiple polygonal grids with differentiation in specific zones/regions, and knolls with macrocells in their summits that arise in the apical third. These characteristics are very different from those found in the majority of Biblidinae and for those reported in the literature for Batesia and Panacea, which belong to the same subtribe as Hamadryas (Ageroniina, now Ageroniini). Chorionic characters support a previously suggested division of the genus (februa, feronia and laodamia groups) and they agree with the phylogenetic proposal based on morphological characters. Our study expands previous morphological work focused on this genus and compiles all the information available to date about the exochorion of Hamadryas, which now includes data for 10 species and that of Ectima thecla thecla, the putative sister group of Hamadryas.
... Thickness, translucent or transparent chorion was found in the most of notodontid subfamilies and the outgroup species-Erebidae, Noctuidae, Nolidae (Dolinskaya, 2010(Dolinskaya, , 2011b(Dolinskaya, , 2014aDolinskaya & Ponomarenko, 2013), Sphingidae, Brahmaeidae (Pljushch & Dolinskaya, 2001) that were examined. Based on the foregoing, I consider this state (thickness, translucent or transparent chorion) is plesiomorphic relative to the solid, opaque chorion. ...
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On the basis of comparative-morphological analysis of 43 genera and 92 species of Palaearctic Notodontidae, as well as the study of the eggs of outgroup species, complexes of characters that are diagnostic, taxonomic or phylogenetic are singled out. It is shown that the egg characteristics are of great taxonomic value at species and generic levels. Some characters are useful for grouping genera. In general, a complex of characters should be used, because different species or genera often share the same characters. Possible apomorphic and plesiomorphic states of the different characters are discussed in relation to the different taxa. The results of this study are discussed with reference to recently published classifications of Notodontidae. As a result of the studies, the keys for identification to the eggs of 43 genera and 92 species of notodontid moths from the Palaearctic region are presented. Reliable diagnostic characters that do not disappear with the injury of eggs or with eggs preserved in alcohol were used. Characters including egg shape, egg and chorion colour, the shape of gnawed holes in eggs when caterpillars hatched, chorionic sculpture, the type of oviposition, foodplants, and geographic distribution of the genera and species were applied. Occasionally, characters that are typical for live eggs, which vary during development, were used. These are characters of egg colour and pattern. The keys are illustrated with photographs made using a digital camera and a scanning electron microscope.
... In recent years, studies on butterfly eggs have increased, using scanning electron microscopy (SEM) and staining techniques with methionine chloride (methylene blue). Among the works of lepidopteran families are those for Noctuidae: Dolinskaya (2010Dolinskaya ( , 2011, Dolinskaya & Geryak (2010), Dolinskaya & Ponomarenko (2013); Hesperiidae: Hernández-Roldán et al. (2012); Pieridae: Eitschberger & Ströhle (1990) and Hernández-Mejía et al. (2013, 2014a,b, 2015; Lycaenidae: Downey and Allyn (1981) and Munguira et al. (2015); Riodinidae: Downey & Allyn (1980); and Nymphalidae: Thomson (1992) and García-Barros & Martín (1995) in Satyrinae, Dell'Erba et al. (2005) in Heliconiini, and Nieves-Uribe et al. (2015, 2016d, 2017a,b) in Biblidinae. Some others have examined aspects of egg size and its variation in an ecological and taxonomic context (e.g. ...
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This is the second exploration, comparison, and analysis of the chorion of species (45 sspp.) of the subfamily Dismorphiinae (Pieridae). This study includes nearly 50% of the species of the subfamily, including six of the seven genera in its two subtribes: Leptidea (Leptideini), Enantia, Pseudopieris, Lieinix, Moschoneura, and Dismorphia (Dismorphiini). The material studied originates from more than three dozen localities in six different countries on three continents (America, Asia, and Europe) and two biogeographical regions, the Palearctic and Neotropical, over the last 20 years. We have corrected and added information regarding several morphological aspects of the chorion. The precision of the citriform configuration and the elimination of the meloniform shape in the egg of Dismorphiini were determined with detailed observations on the maturation of the chorion in the ovarioles where each stage appears in a linear sequence. We discerned that the meloniform states correspond to incompletely differentiated or immature eggs. This was confirmed by the study of new samples of Dismorphia amphione, D. eunoe, and D. lewyi. The chorion of Dismorphiinae is basically plesiomorphic with respect to those of Coliadinae and Pierinae because it lacks several typical synapomorphies of these subfamilies, such as the presence of micro-grid and/or perimicropylar and apex differentiation, respectively. The eggs of each Dismorphiinae genus can be diagnosed by a combination of chorionic features, although sometimes by one or more plesiomorphies or apomorphies in each genus, with respect to the form or character states in axes, ribs, and poles in the grid of the three regions of the egg - two polar regions and one equatorial (basal, medial, and apical). Leptidea and Enantia show the most generalized grid pattern; however, two genera retain several plesiomorphies with respect to the undifferentiated axes or a small number of short axes (Pseudopieris), as well as many equidistant ribs (Lieinix). The chorionic grid of Moschoneura, although practically lacking short axes, shows the fewest number of axes in the entire subfamily (eight aligns it with Pseudopieris). The chorionic grid in Dismorphia is highly diverse, as it shows the most derived states; however, it comprises symplesiomorphies or atavisms in two groups of species, which aligns them closer to Lieinix or Pseudopieris, but we do not take them into account in some cases where they are convergences or structural parallelisms. It seems that the combination of the shape and its length:width ratio is correlated with the alar configuration (design, sexual dimorphism, and coloring patterns) and separates three groups of species in Dismorphia, and often correlates with the number of ribs. This also coincides with the Batesian participation in the number of mimetic complexes in which a subgroup of species and their stenoecy are integrated within the primary forests. Finally, two schemes are presented that synthesize and illustrate the changes or progression of the form and chorionic grid in the genera of the subfamily.
... Posterior a ellos, el empleo de microscopía electrónica de barrido permitió la exploración de la zona perimicropilar en varios taxones: Downey y Allyn (1980,1981,1984) y Munguira et al. (2015) en Riodinidae y Lycaenidae, respectivamente; Kitching (1985) en Danainae; García-Barros y Martin (1995) en Satyrinae; Freitas y Brown (2004) en Nymphalidae; Dell' Erba et al. (2005) y da Silva et al. (2006) en Heliconiini del sur de Brasil. Más recientemente se ha empleado en Noctuidae ( Dolinskaya 2010Dolinskaya , 2011Dolinskaya y Geryak 2010;Dolinskaya y Ponomarenko 2013), y en Hesperiidae ( Hernández-Roldán et al. 2012). Sin embargo, en ninguno de ellos se detalló la conceptualización de las estructuras del exocorion más significativas para su empleo en sistemática, a distintos niveles de la jerarquía taxonómica. ...
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We described and compared the macro and micro exochorionic grid of three species of the genus Colias: C. croceus, C. alfacariensis, and C. lesbia mineira, under methylene blue staining techniques and with the scanning electron microscope (SEM). The size, shapes, as well as diverse aspects of the grid in its region or zones, are different for each species. The differences occur in the micropyle, perimicropylar area, relative thickness between axes and ribs, the arrangement and number of polygons that make up the micro-grid, among other characters we detailed. All these characters are illustrated in drawings and SEM photographs. The results are compared between each species and those of earlier works. © 2018 Southwestern Entomological Society. All rights reserved.