Table 2 - uploaded by Darryl J Bornhop
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Context 1
... determine the stability of NIR-glucosamine, the sample was dissolved in DMSO, pH 5.0 buffer, pH 6.0 buffer, Phosphate Buffered Saline (PBS) buffer (pH 7.3), pH 8.2 buffer, pH 9.0 buffer, and DI water (pH 6.0) independently. The methods for preparation of buffer solutions are listed in Table 2 high glucose) supplemented with 2 mM L-glutamine, 10% FBS, and 50 mg gentamicin sulfate in vented culture flasks (Corning; Corning, NY) at 37 °C and 5% CO 2 . The medium was replaced every three days or as necessary. ...
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... study the stability of NIR-glucosamine, absorbance and fluorescence spectra were Table 2.2. The absorption and fluorescence λ max of NIR-glucosamine shifted ~23 nm and ~22 nm respectively in aqueous solutions compared to solutions made in DMSO due to solvent effect. ...
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... analysis was used to determine whether or not NIR-glucosamine is degraded to free NIR dye in the presence of blood components on a relevant time scale. Following a 0, 4, or 8 hour incubation in whole blood, the elution profile of NIR-glucosamine was compared to that of the NIR-glucosamine and free NIR dye stocks at 782 nm (Table 2.3). NIR-glucosamine and free NIR dye eluted at ~18.1 minutes and ~22.5 minutes, respectively. ...
