This meta-analysis aimed to assess the relational health correlates and intervention effects of partner-focused prayer. Database, backward, and forward searches yielded 1,932 works, resulting in the inclusion of 20 correlational and 17 experimental studies. Standard meta-analytic methods were employed for data analysis. Correlational results showed a significant, small-to-medium, heterogeneous association of partner-focused prayer with relational health (n = 20, r = .215, 95% CI [.174, .255], p < .001, I² = 0.50). Metaregression showed that marital status and age moderated this association, with partner-focused prayer having larger association for older married couples. The results of experiments on partner-focused prayer compared with passive controls showed a significant, medium, homogeneous effect on relational health outcomes (n = 6, d = .474, 95% CI [.263, .686], p < .001, I² = 0.00). Compared with active controls (e.g., thinking positive thoughts about partner or prayer, not specifically for partner), partner-focused prayer showed a significant, medium, homogeneous effect on relational health outcomes (n = 11, d = .582, 95% CI [.405, .759], p < .001, I = 0.00). Of the relational health outcomes, fostering forgiveness (d = .723) and preventing infidelity (d = .742) were the most affected. Overall, partner-focused prayer holds promise as a simple and effective practice to improve relational health outcomes in close relationships. Future research would benefit from focus on partner-focused prayer with adults of all ages, married samples, and lesbian, gay, bisexual, transgender, queer, questioning, and other participants.